Saccharomycopsis praedatoria sp. nov., a predacious yeast isolated from soil and rotten wood in an Amazonian rainforest biome

Journal Article · 31 October 2023 · International Journal of Systematic and Evolutionary Microbiology

DOI: https://doi.org/10.1099/ijsem.0.006125 · OSTI ID:2204325

Santos, Ana Raquel O. ^[1]; Barros, Katharina O. ^[2]; Batista, Thiago M. ^[3]; Souza, Gisele F. L. ^[1]; Alvarenga, Flávia B. M. ^[1]; Abegg, Maxwel A. ^[4]; Sato, Trey K. ^[5]; Hittinger, Chris Todd ^[6]; Lachance, Marc-André  ^[7]; Rosa, Carlos A. ^[1]

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1. Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil
2. Laboratory of Genetics, J. F. Crow Institute for the Study of Evolution, Wisconsin Energy Institute, Center for Genomic Science Innovation, University of Wisconsin-Madison, Madison, Wisconsin, USA, DOE Great Lakes Bioenergy Research Center University of Wisconsin‐Madison, Madison, Wisconsin, USA, Departamento de Microbiologia, ICB, C.P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil
3. Centro de Formação em Ciências Ambientais, Universidade Federal do Sul da Bahia, Porto Seguro, 45.810-000, Brazil
4. Institute of Exact Sciences and Technology (ICET), Federal University of Amazonas (UFAM), Itacoatiara, Amazonas, Brazil
5. DOE Great Lakes Bioenergy Research Center University of Wisconsin‐Madison, Madison, Wisconsin, USA
6. Laboratory of Genetics, J. F. Crow Institute for the Study of Evolution, Wisconsin Energy Institute, Center for Genomic Science Innovation, University of Wisconsin-Madison, Madison, Wisconsin, USA, DOE Great Lakes Bioenergy Research Center University of Wisconsin‐Madison, Madison, Wisconsin, USA
7. Department of Biology, University of Western Ontario, London, ON N6A 5B7, Canada

Three yeast isolates were obtained from soil and rotting wood samples collected in an Amazonian rainforest biome in Brazil. Comparison of the intergenic spacer 5.8S region and the D1/D2 domains of the large subunit rRNA gene showed that the isolates represent a novel species of the genus Saccharomycopsis . A tree inferred from the D1/D2 sequences placed the novel species near a subclade containing Saccharomycopsis lassenensis , Saccharomycopsis fermentans , Saccharomycopsis javanensis , Saccharomycopsis babjevae , Saccharomycopsis schoenii and Saccharomycopsis oosterbeekiorum , but with low bootstrap support. In terms of sequence divergence, the novel species had the highest identity in the D1/D2 domains with Saccharomycopsis capsularis , from which it differed by 36 substitutions. In contrast, a phylogenomic analysis based on 1061 single-copy orthologs for a smaller set of Saccharomycopsis species whose whole genome sequences are available indicated that the novel species represented by strain UFMG-CM-Y6991 is phylogenetically closer to Saccharomycopsis fodiens and Saccharomycopsis sp. TF2021a (= Saccharomycopsis phalluae ). The novel yeast is homothallic and produces asci with one spheroidal ascospore with an equatorial or subequatorial ledge. The name Saccharomycopsis praedatoria sp. nov. is proposed to accommodate the novel species. The holotype of Saccharomycopsis praedatoria is CBS 16589 ^T . The MycoBank number is MB849369. S. praedatoria was able to kill cells of Saccharomyces cerevisiae by means of penetration with infection pegs, a trait common to most species of Saccharomycopsis .

• Sponsoring Organization: USDOE
• Grant/Contract Number: SC0018409
• OSTI ID: 2204325
• Journal Information: International Journal of Systematic and Evolutionary Microbiology, Journal Name: International Journal of Systematic and Evolutionary Microbiology Journal Issue: 10 Vol. 73; ISSN 1466-5026
• Publisher: Society for General MicrobiologyCopyright Statement
• Country of Publication: United Kingdom
• Language: English