Utilization of lignocellulosic hydrolysates for photomixotrophic chemical production in Synechococcus elongatus PCC 7942

Gonzales, Jake N.; Treece, Tanner R.; Mayfield, Stephen P.; Simkovsky, Ryan; Atsumi, Shota

doi:10.1038/s42003-023-05394-w

Title: Utilization of lignocellulosic hydrolysates for photomixotrophic chemical production in Synechococcus elongatus PCC 7942

Journal Article · Mon Oct 09 00:00:00 UTC 2023 · Communications Biology

DOI: https://doi.org/10.1038/s42003-023-05394-w · OSTI ID:2008116

Gonzales, Jake N.; Treece, Tanner R.; Mayfield, Stephen P.;

;

Abstract To meet the need for environmentally friendly commodity chemicals, feedstocks for biological chemical production must be diversified. Lignocellulosic biomass are an carbon source with the potential for effective use in a large scale and cost-effective production systems. Although the use of lignocellulosic biomass lysates for heterotrophic chemical production has been advancing, there are challenges to overcome. Here we aim to investigate the obligate photoautotroph cyanobacterium Synechococcus elongatus PCC 7942 as a chassis organism for lignocellulosic chemical production. When modified to import monosaccharides, this cyanobacterium is an excellent candidate for lysates-based chemical production as it grows well at high lysate concentrations and can fix CO ₂ to enhance carbon efficiency. This study is an important step forward in enabling the simultaneous use of two sugars as well as lignocellulosic lysate. Incremental genetic modifications enable catabolism of both sugars concurrently without experiencing carbon catabolite repression. Production of 2,3-butanediol is demonstrated to characterize chemical production from the sugars in lignocellulosic hydrolysates. The engineered strain achieves a titer of 13.5 g L ⁻¹ of 2,3-butanediol over 12 days under shake-flask conditions. This study can be used as a foundation for industrial scale production of commodity chemicals from a combination of sunlight, CO ₂ , and lignocellulosic sugars.

View Journal Article

Cite

Export

Save

Research Organization:: University of California, San Diego, La Jolla, CA (United States)

Sponsoring Organization:: USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO)

Grant/Contract Number:: EE0008491

OSTI ID:: 2008116

Journal Information:: Communications Biology, Journal Name: Communications Biology Journal Issue: 1 Vol. 6; ISSN 2399-3642

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

References (42)

Metabolic Flux Analysis in Synechocystis Using Isotope Distribution from 13C-Labeled Glucose Yang, C. Metabolic Engineering, Vol. 4, Issue 3 https://doi.org/10.1006/mben.2002.0226	journal	July 2002
SLIC: A Method for Sequence- and Ligation-Independent Cloning Li, Mamie Z.; Elledge, Stephen J. Methods in Molecular Biology https://doi.org/10.1007/978-1-61779-564-0_5	book	January 2012
Current knowledge of the Escherichia coli phosphoenolpyruvate–carbohydrate phosphotransferase system: peculiarities of regulation and impact on growth and product formation Escalante, Adelfo; Salinas Cervantes, Ania; Gosset, Guillermo Applied Microbiology and Biotechnology, Vol. 94, Issue 6 https://doi.org/10.1007/s00253-012-4101-5	journal	May 2012
The cloning and DNA sequence of the gene xylE for xylose-proton symport in Escherichia coli K12. Davis, E. O.; Henderson, P. J. Journal of Biological Chemistry, Vol. 262, Issue 29 https://doi.org/10.1016/S0021-9258(18)47883-0	journal	October 1987
Spacing of the -10 and -35 regions in the tac promoter. Effect on its in vivo activity. Brosius, J.; Erfle, M.; Storella, J. Journal of Biological Chemistry, Vol. 260, Issue 6 https://doi.org/10.1016/S0021-9258(19)83655-4	journal	March 1985
Biosynthesis of 2-aceto-2-hydroxy acids: acetolactate synthases and acetohydroxyacid synthases Chipman, David; Barak, Ze’ev; Schloss, John V. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1385, Issue 2 https://doi.org/10.1016/S0167-4838(98)00083-1	journal	June 1998
Carbon catabolite repression relaxation in Escherichia coli: global and sugar-specific methods for glucose and secondary sugar co-utilization Fox, Kevin J.; Prather, Kristala LJ Current Opinion in Chemical Engineering, Vol. 30 https://doi.org/10.1016/j.coche.2020.05.005	journal	December 2020
Microbial tolerance engineering toward biochemical production: from lignocellulose to products Ling, Hua; Teo, Weisuong; Chen, Binbin Current Opinion in Biotechnology, Vol. 29 https://doi.org/10.1016/j.copbio.2014.03.005	journal	October 2014
A short history of RubisCO: the rise and fall (?) of Nature's predominant CO2 fixing enzyme Erb, Tobias J.; Zarzycki, Jan Current Opinion in Biotechnology, Vol. 49 https://doi.org/10.1016/j.copbio.2017.07.017	journal	February 2018
Metabolic flux phenotyping of secondary metabolism in cyanobacteria Babele, Piyoosh K.; Srivastava, Amit; Young, Jamey D. Trends in Microbiology, Vol. 31, Issue 11 https://doi.org/10.1016/j.tim.2023.05.005	journal	November 2023
Parallel labeling experiments and metabolic flux analysis: Past, present and future methodologies Crown, Scott B.; Antoniewicz, Maciek R. Metabolic Engineering, Vol. 16 https://doi.org/10.1016/j.ymben.2012.11.010	journal	March 2013
A carbon sink pathway increases carbon productivity in cyanobacteria Oliver, John W. K.; Atsumi, Shota Metabolic Engineering, Vol. 29 https://doi.org/10.1016/j.ymben.2015.03.006	journal	May 2015
Engineered xylose utilization enhances bio-products productivity in the cyanobacterium Synechocystis sp. PCC 6803 Lee, Tai-Chi; Xiong, Wei; Paddock, Troy Metabolic Engineering, Vol. 30 https://doi.org/10.1016/j.ymben.2015.06.002	journal	July 2015
2,3 Butanediol production in an obligate photoautotrophic cyanobacterium in dark conditions via diverse sugar consumption McEwen, Jordan T.; Kanno, Masahiro; Atsumi, Shota Metabolic Engineering, Vol. 36 https://doi.org/10.1016/j.ymben.2016.03.004	journal	July 2016
Engineering an Obligate Photoautotrophic Cyanobacterium to Utilize Glycerol for Growth and Chemical Production Kanno, Masahiro; Atsumi, Shota ACS Synthetic Biology, Vol. 6, Issue 1 https://doi.org/10.1021/acssynbio.6b00239	journal	September 2016
Global metabolic rewiring for improved CO2 fixation and chemical production in cyanobacteria Kanno, Masahiro; Carroll, Austin L.; Atsumi, Shota Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms14724	journal	March 2017
Carbon catabolite repression in bacteria: many ways to make the most out of nutrients Görke, Boris; Stülke, Jörg Nature Reviews Microbiology, Vol. 6, Issue 8, p. 613-624 https://doi.org/10.1038/nrmicro1932	journal	August 2008
U1 snRNP regulates cancer cell migration and invasion in vitro Oh, Jung-Min; Venters, Christopher C.; Di, Chao Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-019-13993-7	journal	January 2020
Pathway towards renewable chemicals Haslinger, Kristina; Prather, Kristala L. J. Nature Microbiology, Vol. 2, Issue 12 https://doi.org/10.1038/s41564-017-0071-9	journal	November 2017
Molecular-level driving forces in lignocellulosic biomass deconstruction for bioenergy Petridis, Loukas; Smith, Jeremy C. Nature Reviews Chemistry, Vol. 2, Issue 11 https://doi.org/10.1038/s41570-018-0050-6	journal	October 2018
Options for keeping the food system within environmental limits Springmann, Marco; Clark, Michael; Mason-D’Croz, Daniel Nature, Vol. 562, Issue 7728 https://doi.org/10.1038/s41586-018-0594-0	journal	October 2018
Evolved hexose transporter enhances xylose uptake and glucose/xylose co-utilization in Saccharomyces cerevisiae Reider Apel, Amanda; Ouellet, Mario; Szmidt-Middleton, Heather Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep19512	journal	January 2016
Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria Ungerer, Justin; Pakrasi, Himadri B. Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep39681	journal	December 2016
DMR (deacetylation and mechanical refining) processing of corn stover achieves high monomeric sugar concentrations (230 g L ⁻¹ ) during enzymatic hydrolysis and high ethanol concentrations (>10% v/v) during fermentation without hydrolysate purification or concentration Chen, Xiaowen; Kuhn, Erik; Jennings, Edward W. Energy & Environmental Science, Vol. 9, Issue 4 https://doi.org/10.1039/C5EE03718B	journal	January 2016
Small RNA as global regulator of carbon catabolite repression in Pseudomonas aeruginosa Sonnleitner, E.; Abdou, L.; Haas, D. Proceedings of the National Academy of Sciences, Vol. 106, Issue 51 https://doi.org/10.1073/pnas.0910308106	journal	December 2009
Cyanobacterial conversion of carbon dioxide to 2,3-butanediol Oliver, John W. K.; Machado, Iara M. P.; Yoneda, Hisanari Proceedings of the National Academy of Sciences, Vol. 110, Issue 4 https://doi.org/10.1073/pnas.1213024110	journal	January 2013
The essential gene set of a photosynthetic organism Rubin, Benjamin E.; Wetmore, Kelly M.; Price, Morgan N. Proceedings of the National Academy of Sciences, Vol. 112, Issue 48 https://doi.org/10.1073/pnas.1519220112	journal	October 2015
Circadian gating of cell division in cyanobacteria growing with average doubling times of less than 24 hours. Mori, T.; Binder, B.; Johnson, C. H. Proceedings of the National Academy of Sciences, Vol. 93, Issue 19 https://doi.org/10.1073/pnas.93.19.10183	journal	September 1996
Evolutionary conserved light regulation of Calvin cycle activity by NADPH-mediated reversible phosphoribulokinase/CP12/ glyceraldehyde-3-phosphate dehydrogenase complex dissociation Wedel, Norbert; Soll, Jürgen Proceedings of the National Academy of Sciences, Vol. 95, Issue 16 https://doi.org/10.1073/pnas.95.16.9699	journal	August 1998
Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements Lutz, R. Nucleic Acids Research, Vol. 25, Issue 6, p. 1203-1210 https://doi.org/10.1093/nar/25.6.1203	journal	March 1997
Identification of Genes Conferring Tolerance to Lignocellulose-Derived Inhibitors by Functional Selections in Soil Metagenomes Forsberg, Kevin J.; Patel, Sanket; Witt, Evan Applied and Environmental Microbiology, Vol. 82, Issue 2 https://doi.org/10.1128/AEM.02838-15	journal	November 2015
Engineering Synechococcus elongatus PCC 7942 for Continuous Growth under Diurnal Conditions McEwen, Jordan T.; Machado, Iara M. P.; Connor, Michael R. Applied and Environmental Microbiology, Vol. 79, Issue 5 https://doi.org/10.1128/AEM.03326-12	journal	December 2012
Microbial Cellulose Utilization: Fundamentals and Biotechnology Lynd, L. R.; Weimer, P. J.; van Zyl, W. H. Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577 https://doi.org/10.1128/MMBR.66.3.506-577.2002	journal	September 2002
The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. Jensen, K. F. Journal of Bacteriology, Vol. 175, Issue 11 https://doi.org/10.1128/jb.175.11.3401-3407.1993	journal	June 1993
Organization and regulation of the D-xylose operons in Escherichia coli K-12: XylR acts as a transcriptional activator. Song, S.; Park, C. Journal of bacteriology, Vol. 179, Issue 22 https://doi.org/10.1128/jb.179.22.7025-7032.1997	journal	January 1997
Bioconversion of lignocellulose: inhibitors and detoxification Jönsson, Leif J.; Alriksson, Björn; Nilvebrant, Nils-Olof Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-16	journal	January 2013
Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis Erian, Anna Maria; Gibisch, Martin; Pflügl, Stefan Microbial Cell Factories, Vol. 17, Issue 1 https://doi.org/10.1186/s12934-018-1038-0	journal	November 2018
Hypothesis: Structures, evolution, and ancestor of glucose kinases in the hexokinase family Kawai, Shigeyuki; Mukai, Takako; Mori, Shigetarou Journal of Bioscience and Bioengineering, Vol. 99, Issue 4 https://doi.org/10.1263/jbb.99.320	journal	April 2005
2016 Billion-Ton Report: Advancing Domestic Resources for a Thriving Bioeconomy Langholtz, M. H.; Stokes, B. J.; Eaton, L. M. https://doi.org/10.2172/1271651 DOE/EE-1440	report	July 2016
Cyanobacteria as a Platform for Biofuel Production Nozzi, Nicole E.; Oliver, John W. K.; Atsumi, Shota Frontiers in Bioengineering and Biotechnology, Vol. 1 https://doi.org/10.3389/fbioe.2013.00007	journal	January 2013
Characterizing Photosynthetic Biofuel Production: Isotopically Non-Stationary 13C Metabolic Flux Analysis on Limonene Producing Synechococcus sp. PCC 7002 Newman, Darrian M.; Sake, Cara L.; Metcalf, Alexander J. Frontiers in Energy Research, Vol. 10 https://doi.org/10.3389/fenrg.2022.896668	journal	July 2022
Biofuels from algae: challenges and potential Hannon, Michael; Gimpel, Javier; Tran, Miller Biofuels, Vol. 1, Issue 5 https://doi.org/10.4155/bfs.10.44	journal	September 2010

Similar Records

Metabolic engineering of non-pathogenic microorganisms for 2,3-butanediol production

Journal Article · Wed Jul 21 00:00:00 UTC 2021 · Applied Microbiology and Biotechnology · OSTI ID:1834453

Lee, Jae Won; Lee, Ye-Gi; Jin, Yong-Su; +1 more

Metabolic engineering of Caldicellulosiruptor bescii for 2,3-butanediol production from unpretreated lignocellulosic biomass and metabolic strategies for improving yields and titers

Journal Article · Wed Jan 24 04:00:00 UTC 2024 · Applied and Environmental Microbiology · OSTI ID:2580000

Tanwee, Tania; Lipscomb, Gina; Vailionis, Jason; +8 more

Biomass fermentation technology, Project No. 1. Final performance report. Cooperative research and training in biomass conversion and utilization

Technical Report · Thu Mar 01 04:00:00 UTC 1984 · OSTI ID:6907307

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Bacterial synthetic biology
Metabolic engineering

Title: Utilization of lignocellulosic hydrolysates for photomixotrophic chemical production in Synechococcus elongatus PCC 7942

Citation Formats

References (42)

Similar Records

Related Subjects