In vitro maturation of NiSOD reveals a role for cytoplasmic histidine in processing and metalation

Basak, Priyanka; Cabelli, Diane E.; Chivers, Peter T.; Farquhar, Erik R.; Maroney, Michael J.

doi:10.1093/mtomcs/mfad054

Title: In vitro maturation of NiSOD reveals a role for cytoplasmic histidine in processing and metalation

Journal Article · 17 September 2023 · Metallomics (Online)

DOI: https://doi.org/10.1093/mtomcs/mfad054 · OSTI ID:2007526

Basak, Priyanka ^[1]; Cabelli, Diane E. ^[2];

^[3];

^[4];

^[1]

University of Massachusetts, Amherst, MA (United States)
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Durham University (United Kingdom)
Brookhaven National Laboratory (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II)

The importance of cellular low molecular weight (LMW) ligands in metalloenzyme maturation is largely unexplored. Maturation of NiSOD requires post-translational N-terminal processing of the proenzyme, SodN, by its cognate protease, SodX. Here we provide evidence for the participation of L-histidine in the protease-dependent maturation of Nickel-dependent Superoxide Dismutase (NiSOD) from Streptomyces coelicolor. Furthermore, in vitro studies using purified proteins cloned from S. coelicolor and overexpressed in E. coli support a model where a ternary complex formed between the substrate (SodN), the protease (SodX) and L-Histidine creates a novel Ni-binding site that is capable of the N-terminal processing of SodN and specifically incorporates Ni into the apo-NiSOD product. Thus, L-Histidine serves many of the functions associated with a metallochaperone or, conversely, eliminates the need for a metallochaperone in NiSOD maturation.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science (BSS); National Institutes of Health (NIH)

Grant/Contract Number:: SC0012704

OSTI ID:: 2007526

Report Number(s):: BNL--224865-2023-JAAM

Journal Information:: Metallomics (Online), Journal Name: Metallomics (Online) Journal Issue: 11 Vol. 15; ISSN 1756-591X

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
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enzyme maturation

Title: In vitro maturation of NiSOD reveals a role for cytoplasmic histidine in processing and metalation

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