A high-throughput structural system biology approach to increase structure representation of proteins from Clostridioides difficile

Rosas-Lemus, Monica; Dey, Supratim; Minasov, George; Tan, Kemin; Anderson, Spencer M.; Brunzelle, Joseph; Nocadello, Salvatore; Shabalin, Ivan; Filippova, Ekaterina; Halavaty, Andrei; Kim, Youngchang; Maltseva, Natalia; Osipiuk, Jerzy; Minor, Wladek; Joachimiak, Andrzej; Savchenko, Alexei; Anderson, Wayne F.; Satchell, Karla J. F.; Newton, ed., Irene L. G.

doi:10.1128/MRA.00507-23

Title: A high-throughput structural system biology approach to increase structure representation of proteins from Clostridioides difficile

Journal Article · 18 October 2023 · Microbiology Resource Announcements

DOI: https://doi.org/10.1128/MRA.00507-23 · OSTI ID:2005023

Rosas-Lemus, Monica ^[1]; Dey, Supratim ^[1]; Minasov, George ^[1];

^[2]; Anderson, Spencer M. ^[3]; Brunzelle, Joseph ^[3]; Nocadello, Salvatore ^[1]; Shabalin, Ivan ^[4]; Filippova, Ekaterina ^[1]; Halavaty, Andrei ^[1]; Kim, Youngchang ^[2]; Maltseva, Natalia ^[2]; Osipiuk, Jerzy ^[2]; Minor, Wladek ^[4]; Joachimiak, Andrzej ^[2];

^[5]; Anderson, Wayne F. ^[6];

^[1]; Newton, ed., Irene L. G.

Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA, Center for Structural Biology of Infectious Diseases, Chicago, Illinois, USA
Center for Structural Biology of Infectious Diseases, Chicago, Illinois, USA, Consortium for Advanced Science and Engineering, University of Chicago, Chicago, Illinois, USA, Structural Biology Center, X-ray Science Division, Argonne National Laboratory, Argonne, Illinois, USA
Northwestern Synchrotron Research Center, Life Sciences Collaborative Access Team, Northwestern University, Argonne, Illinois, USA
Center for Structural Biology of Infectious Diseases, Chicago, Illinois, USA, Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, USA
Center for Structural Biology of Infectious Diseases, Chicago, Illinois, USA, Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
Center for Structural Biology of Infectious Diseases, Chicago, Illinois, USA, Department of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA

ABSTRACT Clostridioides difficile causes life-threatening gastrointestinal infections. It is a high-risk pathogen due to a lack of effective treatments, antimicrobial resistance, and a poorly conserved genomic core. Herein, we report 30 X-ray structures from a structure genomics pipeline spanning 13 years, representing 10.2% of the X-ray structures for this important pathogen.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 2005023

Journal Information:: Microbiology Resource Announcements, Journal Name: Microbiology Resource Announcements Journal Issue: 10 Vol. 12; ISSN 2576-098X

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
clostridiodes
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pipeline
protein structure-function
structural genomics
x-ray

Title: A high-throughput structural system biology approach to increase structure representation of proteins from Clostridioides difficile

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