Bacterium-enabled transient gene activation by artificial transcription factors for resolving gene regulation in maize

Zhao, Mingxia; Peng, Zhao; Qin, Yang; Tamang, Tej Man; Zhang, Ling; Tian, Bin; Chen, Yueying; Liu, Yan; Zhang, Junli; Lin, Guifang; Zheng, Huakun; He, Cheng; Lv, Kaiwen; Klaus, Alina; Marcon, Caroline; Hochholdinger, Frank; Trick, Harold N.; Liu, Yunjun; Cho, Myeong-Je; Park, Sunghun; Wei, Hairong; Zheng, Jun; White, Frank F.; Liu, Sanzhen

doi:10.1093/plcell/koad155

Title: Bacterium-enabled transient gene activation by artificial transcription factors for resolving gene regulation in maize

Journal Article · 25 May 2023 · The Plant Cell

DOI: https://doi.org/10.1093/plcell/koad155 · OSTI ID:1993526

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Abstract Understanding gene regulatory networks is essential to elucidate developmental processes and environmental responses. Here, we studied regulation of a maize (Zea mays) transcription factor gene using designer transcription activator-like effectors (dTALes), which are synthetic Type III TALes of the bacterial genus Xanthomonas and serve as inducers of disease susceptibility gene transcription in host cells. The maize pathogen Xanthomonas vasicola pv. vasculorum was used to introduce 2 independent dTALes into maize cells to induced expression of the gene glossy3 (gl3), which encodes a MYB transcription factor involved in biosynthesis of cuticular wax. RNA-seq analysis of leaf samples identified, in addition to gl3, 146 genes altered in expression by the 2 dTALes. Nine of the 10 genes known to be involved in cuticular wax biosynthesis were upregulated by at least 1 of the 2 dTALes. A gene previously unknown to be associated with gl3, Zm00001d017418, which encodes aldehyde dehydrogenase, was also expressed in a dTALe-dependent manner. A chemically induced mutant and a CRISPR-Cas9 mutant of Zm00001d017418 both exhibited glossy leaf phenotypes, indicating that Zm00001d017418 is involved in biosynthesis of cuticular waxes. Bacterial protein delivery of dTALes proved to be a straightforward and practical approach for the analysis and discovery of pathway-specific genes in maize.

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Research Organization:: Univ. of Nebraska, Lincoln, NE (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: SC0023138

OSTI ID:: 1993526

Journal Information:: The Plant Cell, Journal Name: The Plant Cell Journal Issue: 8 Vol. 35; ISSN 1040-4651

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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