Energetic robustness to large scale structural fluctuations in a photosynthetic supercomplex

Harris, Dvir; Toporik, Hila; Schlau-Cohen, Gabriela S.; Mazor, Yuval

doi:10.1038/s41467-023-40146-8

Title: Energetic robustness to large scale structural fluctuations in a photosynthetic supercomplex

Journal Article · 01 August 2023 · Nature Communications

DOI: https://doi.org/10.1038/s41467-023-40146-8 · OSTI ID:1993523

Harris, Dvir; Toporik, Hila;

;

Abstract Photosynthetic organisms transport and convert solar energy with near-unity quantum efficiency using large protein supercomplexes held in flexible membranes. The individual proteins position chlorophylls to tight tolerances considered critical for fast and efficient energy transfer. The variability in protein organization within the supercomplexes, and how efficiency is maintained despite variability, had been unresolved. Here, we report on structural heterogeneity in the 2-MDa cyanobacterial PSI-IsiA photosynthetic supercomplex observed using Cryo-EM, revealing large-scale variances in the positions of IsiA relative to PSI. Single-molecule measurements found efficient IsiA-to-PSI energy transfer across all conformations, along with signatures of transiently decoupled IsiA. Structure based calculations showed that rapid IsiA-to-PSI energy transfer is always maintained, and even increases by three-fold in rare conformations via IsiA-specific chls. We postulate that antennae design mitigates structural fluctuations, providing a mechanism for robust energy transfer in the flexible membrane.

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Research Organization:: Arizona State Univ., Tempe, AZ (United States); Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB)

Grant/Contract Number:: SC0018097; SC0022956

OSTI ID:: 1993523

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 14; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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