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Title: Cell‐ and development‐specific degradation controls the levels of mixed‐linkage glucan in sorghum leaves

Journal Article · · The Plant Journal
DOI: https://doi.org/10.1111/tpj.16376 · OSTI ID:1992542
ORCiD logo [1];  [2];  [3];  [2];  [2];  [3]; ORCiD logo [1]
  1. Great Lakes Bioenergy Research Center Michigan State University East Lansing Michigan 48824 USA, MSU‐DOE Plant Research Laboratory Michigan State University East Lansing Michigan 48824 USA, Department of Plant Biology Michigan State University East Lansing Michigan 48824 USA
  2. Great Lakes Bioenergy Research Center Michigan State University East Lansing Michigan 48824 USA, MSU‐DOE Plant Research Laboratory Michigan State University East Lansing Michigan 48824 USA
  3. Great Lakes Bioenergy Research Center Michigan State University East Lansing Michigan 48824 USA, Department of Biochemistry and Biophysics Texas A&,M University College Station Texas 77845 USA
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SUMMARY Mixed‐linkage glucan (MLG) is a component of the cell wall (CW) of grasses and is composed of glucose monomers linked by β‐1,3 and β‐1,4 bonds. MLG is believed to have several biological functions, such as the mobilizable storage of carbohydrates and structural support of the CW. The extracellular levels of MLG are largely controlled by rates of synthesis mediated by cellulose synthase‐like (CSL) enzymes, and turnover by lichenases. Economically important crops like sorghum accumulate MLG to variable levels during development. While in sorghum, like other grasses, there is one major MLG synthase (CSLF6), the identity of lichenases is yet unknown. To fill this gap, we identified three sorghum lichenases (SbLCH1‐3) and characterized them in leaves in relation to the expression of SbCSLF6 , and the abundance of MLG and starch. We established that SbLCH1‐3 are secreted to the apoplast, consistent with a role of degrading MLG extracellularly. Furthermore, while SbCSLF6 expression was associated with cell development, the SbLCH genes exhibited distinct development‐, cell‐type‐specific and diel‐regulated expression. Therefore, our study identifies three functional sorghum MLG lichenases and highlights that MLG accumulation in sorghum leaves is likely controlled by the activity of lichenases that tune MLG levels, possibly to suit distinct cell and developmental needs in planta . These findings have important implications for improving the growth, yield, and composition of sorghum as a feedstock.

Research Organization:
Michigan State Univ., East Lansing, MI (United States); Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Organization:
USDOE; USDOE Office of Science (SC)
Grant/Contract Number:
FG02-91ER20021; SC0018409
OSTI ID:
1992542
Journal Information:
The Plant Journal, Journal Name: The Plant Journal Journal Issue: 2 Vol. 116; ISSN 0960-7412
Publisher:
Wiley-BlackwellCopyright Statement
Country of Publication:
United Kingdom
Language:
English

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Plant Sciences