Overexpression of a gibberellin 20-oxidase gene in poplar xylem led to an increase in the size of nanocellulose fibrils and improved paper properties

Peng, Xiaopeng; Tong, Botong; Lee, Jongcheol; Wang, Kun; Yu, Xiaojuan; Huang, Xiong; Wen, Jialong; Makarem, Mohamadamin; Pang, Hongying; Hinjan, Subin; Yan, Xiaojing; Yao, Shuangquan; Lu, Fachuang; Wang, Baichen; Peng, Feng; Ralph, John; Kim, Seong H.; Sederoff, Ronald R.; Li, Quanzi

doi:10.1016/j.carbpol.2023.120959

Title: Overexpression of a gibberellin 20-oxidase gene in poplar xylem led to an increase in the size of nanocellulose fibrils and improved paper properties

Journal Article · 27 April 2023 · Carbohydrate Polymers

DOI: https://doi.org/10.1016/j.carbpol.2023.120959 · OSTI ID:1992369

Peng, Xiaopeng ^[1]; Tong, Botong ^[2]; Lee, Jongcheol ^[3]; Wang, Kun ^[4]; Yu, Xiaojuan ^[4]; Huang, Xiong ^[1]; Wen, Jialong ^[4]; Makarem, Mohamadamin ^[3]; Pang, Hongying ^[1]; Hinjan, Subin ^[5]; Yan, Xiaojing ^[1]; Yao, Shuangquan ^[6]; Lu, Fachuang ^[7]; Wang, Baichen ^[8]; Peng, Feng ^[4]; Ralph, John ^[7]; Kim, Seong H. ^[3]; Sederoff, Ronald R. ^[9]; Li, Quanzi ^[1]

Chinese Academy of Forestry, Beijing (China)
Chinese Academy of Forestry, Beijing (China); Northeast Forestry University, Harbin (China)
Pennsylvania State University, University Park, PA (United States)
Beijing Forestry University (China)
Bangkok Cordyceps Co., Ltd, Pathumthani (Thailand)
Guangxi University, Nanning (China)
University of Wisconsin, Madison, WI (United States)
Chinese Academy of Sciences (CAS), Beijing (China)
Northeast Forestry University, Harbin (China); North Carolina State University, Raleigh, NC (United States)

Cellulose, the major component of secondary cell walls, is the most abundant renewable long-chain polymer on earth. Nanocellulose has become a prominent nano-reinforcement agent for polymer matrices in various industries. We report the generation of transgenic hybrid poplar overexpressing the Arabidopsis gibberellin 20-oxidase1 gene driven by a xylem-specific promoter to increase gibberellin (GA) biosynthesis in wood. X-ray diffraction (XRD) and sum frequency generation spectroscopic (SFG) analyses showed that cellulose in transgenic trees was less crystalline, but the crystal size was larger. The nanocellulose fibrils prepared from transgenic wood had an increased size compared to those from wild type. When such fibrils were used as a reinforcing agent in sheet paper preparation, the mechanical strength of the paper was significantly enhanced. Engineering the GA pathway can therefore affect nanocellulose properties, providing a new strategy for expanding nanocellulose applications.

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Research Organization:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF); Pennsylvania State Univ., University Park, PA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Contributing Organization:: North Carolina State University; Univ. of Wisconsin

Grant/Contract Number:: SC0018409; SC0001090

OSTI ID:: 1992369

Alternate ID(s):: OSTI ID: 2345026

Journal Information:: Carbohydrate Polymers, Vol. 314; ISSN 0144-8617

Publisher:: Applied Science DirectCopyright Statement

Country of Publication:: United States

Language:: English

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