Structures and Mechanisms of a Novel Bacterial Transport System for Fatty Acids

Zhai, Liting; Chou, Jonathan Chiu‐Chun; Oo, Hannah; Dassama, Laura M. K.

doi:10.1002/cbic.202300156

Title: Structures and Mechanisms of a Novel Bacterial Transport System for Fatty Acids

Journal Article · 05 July 2023 · ChemBioChem: a European journal of chemical biology

DOI: https://doi.org/10.1002/cbic.202300156 · OSTI ID:1988324

Zhai, Liting ^[1]; Chou, Jonathan Chiu‐Chun ^[2]; Oo, Hannah ^[2];

^[3]

Department of Chemistry and Sarafan ChEM−H Institute Stanford University Stanford CA 94305 USA, Current address: School of Life Sciences The Chinese University of Hong Kong, Shatin New Territories Hong Kong China
Department of Chemistry and Sarafan ChEM−H Institute Stanford University Stanford CA 94305 USA
Department of Chemistry and Sarafan ChEM−H Institute Stanford University Stanford CA 94305 USA, Department of Microbiology and Immunology Stanford School of Medicine Stanford CA 94305 USA

Abstract Bacterial acquisition of metabolites is largely facilitated by transporters with unique substrate scopes. The tripartite ATP‐independent periplasmic (TRAP) transporters comprise a large family of bacterial proteins that facilitate the uptake of a variety of small molecules. It has been reported that some TRAP systems encode a fourth protein, the T component. The T‐component, or TatT, is predicted to be a periplasmic‐facing lipoprotein that enables the uptake of metabolites from the outer membrane. However, no substrates were revealed for any TatT and their functional role(s) remained enigmatic. We recently identified a homolog in Methylococcus capsulatus that binds to sterols, and herein, we report two additional homologs that demonstrate a preference for long‐chain fatty acids. Our bioinformatics, quantitative analyses of protein‐ligand interactions, and high‐resolution crystal structures suggest that TatTs might facilitate the trafficking of hydrophobic or lipophilic substrates and represent a new class of bacterial lipid and fatty acid transporters.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: NONE; AC02-76SF00515

OSTI ID:: 1988324

Alternate ID(s):: OSTI ID: 1993344; OSTI ID: 1998183

Journal Information:: ChemBioChem: a European journal of chemical biology, Journal Name: ChemBioChem: a European journal of chemical biology Journal Issue: 15 Vol. 24; ISSN 1439-4227

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: France

Language:: English

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