Dynamic Phaeodactylum tricornutum exometabolites shape surrounding bacterial communities

Brisson, Vanessa; Swink, Courtney; Kimbrel, Jeffrey; Mayali, Xavier; Samo, Ty; Kosina, Suzanne M.; Thelen, Michael; Northen, Trent R.; Stuart, Rhona K.

doi:10.1111/nph.19051

Title: Dynamic Phaeodactylum tricornutum exometabolites shape surrounding bacterial communities

Journal Article · 10 June 2023 · New Phytologist

DOI: https://doi.org/10.1111/nph.19051 · OSTI ID:1984534

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Physical and Life Sciences Directorate Lawrence Livermore National Laboratory Livermore CA 94550 USA
Environmental Genomics and Systems Biology Division Lawrence Berkeley National Laboratory Berkeley CA 94720 USA
Environmental Genomics and Systems Biology Division Lawrence Berkeley National Laboratory Berkeley CA 94720 USA, The DOE Joint Genome Institute Lawrence Berkeley National Laboratory Berkeley CA 94720 USA

Summary Roles of different ecological classes of algal exometabolites in regulating microbial community composition are not well understood. Here, we identify exometabolites from the model diatom Phaeodactylum tricornutum and demonstrate their potential to influence bacterial abundances. We profiled exometabolites across a time course of axenic algal growth using liquid chromatography–tandem mass spectrometry. We then investigated growth of 12 bacterial isolates on individual‐identified exometabolites. Lastly, we compared responses of a P. tricornutum ‐adapted enrichment community to additions of two contrasting metabolites: selective growth substrate 4‐hydroxybenzoic acid and putative signaling/facilitator molecule lumichrome. We identified 50 P. tricornutum metabolites and found distinct temporal accumulation patterns. Two exometabolites (of 12 tested) supported growth of distinct subsets of bacterial isolates. While algal exudates and algal presence drove similar changes in community composition compared with controls, exogenous 4‐hydroxybenzoic acid addition promoted increased abundances of taxa that utilized it in isolation, and also revealed the importance of factors relating to algal presence in regulating community composition. This work demonstrates that secretion of selective bacterial growth substrates represents one mechanism by which algal exometabolites can influence bacterial community composition and illustrates how the algal exometabolome has the potential to modulate bacterial communities as a function of algal growth.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE; USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; AC52-07NA27344

OSTI ID:: 1984534

Report Number(s):: LLNL-JRNL-836013

Journal Information:: New Phytologist, Journal Name: New Phytologist Journal Issue: 4 Vol. 239; ISSN 0028-646X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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algal-bacterial interactions
exometabolites
lumichrome
microbiome

Title: Dynamic Phaeodactylum tricornutum exometabolites shape surrounding bacterial communities

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References (49)

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