SARS-CoV-2 Beta variant infection elicits potent lineage-specific and cross-reactive antibodies

Reincke, S. Momsen; Yuan, Meng; Kornau, Hans-Christian; Corman, Victor M.; van Hoof, Scott; Sánchez-Sendin, Elisa; Ramberger, Melanie; Yu, Wenli; Hua, Yuanzi; Tien, Henry; Schmidt, Marie Luisa; Schwarz, Tatjana; Jeworowski, Lara Maria; Brandl, Sarah E.; Rasmussen, Helle Foverskov; Homeyer, Marie A.; Stöffler, Laura; Barner, Martin; Kunkel, Désirée; Huo, Shufan; Horler, Johannes; von Wardenburg, Niels; Kroidl, Inge; Eser, Tabea M.; Wieser, Andreas; Geldmacher, Christof; Hoelscher, Michael; Gänzer, Hannes; Weiss, Günter; Schmitz, Dietmar; Drosten, Christian; Prüss, Harald; Wilson, Ian A.; Kreye, Jakob

doi:10.1126/science.abm5835

Title: SARS-CoV-2 Beta variant infection elicits potent lineage-specific and cross-reactive antibodies

Journal Article · 24 January 2022 · Science

DOI: https://doi.org/10.1126/science.abm5835 · OSTI ID:1982958

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Freie Universität, Berlin (Germany); Humboldt University of Berlin (Germany); German Center for Neurodegenerative Diseases (DZNE), Berlin (Germany); Helmholtz Innovation Laboratory BaoBab (Brain Antibody-omics and B-cell Laboratory), Berlin (Germany)
Scripps Research Institute, La Jolla, CA (United States)
Freie Universität, Berlin (Germany); Humboldt University of Berlin (Germany); German Center for Neurodegenerative Diseases (DZNE), Berlin (Germany)
Freie Universität, Berlin (Germany); Humboldt University of Berlin (Germany); German Centre for Infection Research (DZIF), Berlin (Germany); Labor Berlin-Charité Vivantes GmbH, Berlin (Germany)
German Center for Neurodegenerative Diseases (DZNE), Berlin (Germany); Helmholtz Innovation Laboratory BaoBab (Brain Antibody-omics and B-cell Laboratory), Berlin (Germany)
Freie Universität, Berlin (Germany); Humboldt University of Berlin (Germany); German Centre for Infection Research (DZIF), Berlin (Germany)
Helmholtz Innovation Laboratory BaoBab (Brain Antibody-omics and B-cell Laboratory), Berlin (Germany)
Charité–Universitätsmedizin, Berlin (Germany)
Freie Universität, Berlin (Germany); Humboldt University of Berlin (Germany)
Medical Center of the University of Munich (LMU) (Germany); German Center for Infection Research (DZIF), Munich (Germany)
BKH Schwaz (Austria)
Medical University of Innsbruck (Austria)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Beta variant of concern (VOC) resists neutralization by major classes of antibodies from COVID-19 patients and vaccinated individuals. In this study, serum of Beta-infected patients revealed reduced cross-neutralization of wild-type virus. From these patients, we isolated Beta-specific and cross-reactive receptor-binding domain (RBD) antibodies. The Beta-specificity results from recruitment of VOC-specific clonotypes and accommodation of mutations present in Beta and Omicron into a major antibody class that is normally sensitive to these mutations. The Beta-elicited cross-reactive antibodies share genetic and structural features with wild type–elicited antibodies, including a public VH1-58 clonotype that targets the RBD ridge. These findings advance our understanding of the antibody response to SARS-CoV-2 shaped by antigenic drift, with implications for design of next-generation vaccines and therapeutics.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source (ALS)

Sponsoring Organization:: Austrian Science Fund (FWF); Bill and Melinda Gates Foundation; German Research Foundation (DFG); National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; AC02-76SF00515

OSTI ID:: 1982958

Journal Information:: Science, Journal Name: Science Journal Issue: 6582 Vol. 375; ISSN 0036-8075

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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