Escaping the fate of Sisyphus: assessing resistome hybridization baits for antimicrobial resistance gene capture
[1];
[3];
[3];
- University of Georgia, Athens, GA (United States); OSTI
- University of Georgia, Athens, GA (United States); University of Georgia, Aiken, SC (United States). Savannah River Ecology Laboratory
- University of Georgia, Athens, GA (United States)
- Daicel Arbor Biosciences, Ann Arbor, MI (United States)
- University of Georgia, Aiken, SC (United States). Savannah River Ecology Laboratory; University of Georgia, Athens, GA (United States)
- Centers for Disease Control and Prevention (CDC), Atlanta, GA (United States)
- University of Georgia, Athens, GA (United States); Emory University, Oxford, GA (United States)
- US Department of Agriculture (USDA), Athens, GA (United States)
Finding, characterizing and monitoring reservoirs for antimicrobial resistance (AMR) is vital to protecting public health. Hybridization capture baits are an accurate, sensitive and cost-effective technique used to enrich and characterize DNA sequences of interest, including antimicrobial resistance genes (ARGs), in complex environmental samples. We demonstrate the continued utility of a set of 19 933 hybridization capture baits designed from the Comprehensive Antibiotic Resistance Database (CARD)v1.1.2 and Pathogenicity Island Database (PAIDB)v2.0, targeting 3565 unique nucleotide sequences that confer resistance. We demonstrate the efficiency of our bait set on a custom-made resistance mock community and complex environmental samples to increase the proportion of on-target reads as much as >200-fold. However, keeping pace with newly discovered ARGs poses a challenge when studying AMR, because novel ARGs are continually being identified and would not be included in bait sets designed prior to discovery. Here we provide imperative information on how our bait set performs against CARDv3.3.1, as well as a generalizable approach for deciding when and how to update hybridization capture bait sets. This research encapsulates the full life cycle of baits for hybridization capture of the resistome from design and validation (both in silico and in vitro) to utilization and forecasting updates and retirement.
- Research Organization:
- University of Georgia, Athens, GA (United States)
- Sponsoring Organization:
- USDOE Office of Environmental Management (EM); US Department of Agriculture (USDA); Centers for Disease Control and Prevention (CDC)
- Grant/Contract Number:
- FC09-07SR22506
- OSTI ID:
- 1980609
- Journal Information:
- Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 12 Vol. 23; ISSN 1462-2912
- Publisher:
- WileyCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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