Engineering stability, longevity, and miscibility of microtubule-based active fluids

Chandrakar, Pooja; Berezney, John; Lemma, Bezia; Hishamunda, Bernard; Berry, Angela; Wu, Kun-Ta; Subramanian, Radhika; Chung, Johnson; Needleman, Daniel; Gelles, Jeff; Dogic, Zvonimir

doi:10.1039/d1sm01289d

Title: Engineering stability, longevity, and miscibility of microtubule-based active fluids

Journal Article · 17 January 2022 · Soft Matter

DOI: https://doi.org/10.1039/d1sm01289d · OSTI ID:1978827

^[1]; Berezney, John ^[2]; Lemma, Bezia ^[3]; Hishamunda, Bernard ^[2]; Berry, Angela ^[4];

^[5]; Subramanian, Radhika ^[6]; Chung, Johnson ^[2]; Needleman, Daniel ^[7];

^[2];

^[8]

Brandeis Univ., Waltham, MA (United States); Univ. of California, Santa Barbara, CA (United States); OSTI
Brandeis Univ., Waltham, MA (United States)
Brandeis Univ., Waltham, MA (United States); Univ. of California, Santa Barbara, CA (United States); Harvard Univ., Cambridge, MA (United States)
Hampton Univ., Hampton, VA (United States). School of Pharmacy; Brandeis Univ., Waltham, MA (United States)
Brandeis Univ., Waltham, MA (United States); Worcester Polytechnic Institute, MA (United States)
Massachusetts General Hospital, Boston, MA (United States)
Harvard Univ., Cambridge, MA (United States); Flatiron Institute, New York, NY (United States)
Brandeis Univ., Waltham, MA (United States); Univ. of California, Santa Barbara, CA (United States)

Microtubule-based active matter provides insight into the self-organization of motile interacting constituents. In this work, we describe several formulations of microtubule-based 3D active isotropic fluids. Dynamics of these fluids is powered by three types of kinesin motors: a processive motor, a non-processive motor, and a motor which is permanently linked to a microtubule backbone. Another modification uses a specific microtubule crosslinker to induce bundle formation instead of a non-specific polymer depletant. In comparison to the already established system, each formulation exhibits distinct properties. These developments reveal the temporal stability of microtubule-based active fluids while extending their reach and the applicability.

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Research Organization:: Univ. of California, Santa Barbara, CA (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0019733

OSTI ID:: 1978827

Journal Information:: Soft Matter, Journal Name: Soft Matter Journal Issue: 9 Vol. 18; ISSN 1744-683X

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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