Protein-based models offer mechanistic insight into complex nickel metalloenzymes

Treviño, Regina E.; Shafaat, Hannah S.

doi:10.1016/j.cbpa.2021.102110

Title: Protein-based models offer mechanistic insight into complex nickel metalloenzymes

Journal Article · 31 January 2022 · Current Opinion in Chemical Biology

DOI: https://doi.org/10.1016/j.cbpa.2021.102110 · OSTI ID:1976913

Treviño, Regina E. ^[1];

^[2]

The Ohio State Univ., Columbus, OH (United States); OSTI
The Ohio State Univ., Columbus, OH (United States)

There are ten nickel enzymes found across biological systems, each with a distinct active site and reactivity that spans reductive, oxidative, and redox–neutral processes. We focus on the reductive enzymes, which catalyze reactions that are highly germane to the modern-day climate crisis: [NiFe] hydrogenase, carbon monoxide dehydrogenase, acetyl coenzyme A synthase, and methyl coenzyme M reductase. The current mechanistic understanding of each enzyme system is reviewed along with existing knowledge gaps, which are addressed through the development of protein-derived models, as described here. Finally, this opinion is intended to highlight the advantages of using robust protein scaffolds for modeling multiscale contributions to reactivity and inspire the development of novel artificial metalloenzymes for other small molecule transformations.

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Research Organization:: The Ohio State Univ., Columbus, OH (United States)

Sponsoring Organization:: Alfred P. Sloan Foundation; National Science Foundation (NSF); USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: SC0018020

OSTI ID:: 1976913

Journal Information:: Current Opinion in Chemical Biology, Journal Name: Current Opinion in Chemical Biology Journal Issue: C Vol. 67; ISSN 1367-5931

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
Acetyl coenzyme A synthase
Artificial metalloenzymes
Biochemistry & Molecular biology
Biophysics
Carbon monoxide dehydrogenase
Catalytic mechanism
Methyl coenzyme M reductase
Nickel
Protein engineering
[NiFe] hydrogenase

Title: Protein-based models offer mechanistic insight into complex nickel metalloenzymes

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