The pGinger Family of Expression Plasmids

Journal Article · 14 June 2023 · Microbiology Spectrum

DOI: https://doi.org/10.1128/spectrum.00373-23 · OSTI ID:1974645

Pearson, Allison N. ^[1]; Thompson, Mitchell G. ^[2]; Kirkpatrick, Liam D. ^[2]; Ho, Cindy ^[3]; Vuu, Khanh M. ^[2]; Waldburger, Lucas M. ^[4]; Keasling, Jay D. ^[5]; Shih, Patrick M.  ^[6]; Bond, ed., Daniel R.

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1. Joint BioEnergy Institute, Emeryville, California, USA, Biological Systems &, Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA, Department of Plant and Microbial Biology, University of California, Berkeley, California, USA
2. Joint BioEnergy Institute, Emeryville, California, USA, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
3. Joint BioEnergy Institute, Emeryville, California, USA, Biological Systems &, Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
4. Joint BioEnergy Institute, Emeryville, California, USA, Biological Systems &, Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA, Department of Bioengineering, University of California, Berkeley, California, USA
5. Joint BioEnergy Institute; Emeryville; California; USA; Biological Systems & Engineering Division; Lawrence Berkeley National Laboratory; Berkeley; California; USA; Department of Chemical and Biomolecular Engineering; University of California; Berkeley; California; USA; The Novo Nordisk Foundation Center for Biosustainability; Technical University of Denmark; Kongens Lyngby; Denmark; Center for Synthetic Biochemistry; Institute for Synthetic Biology; Shenzhen Institutes for Advanced Technologies; Shenzhen; China
6. Joint BioEnergy Institute, Emeryville, California, USA, Department of Plant and Microbial Biology, University of California, Berkeley, California, USA, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA, Innovative Genomics Institute, University of California, Berkeley, California, USA

Metabolic engineering and synthetic biology are predicated on the precise control of gene expression. As synthetic biology expands beyond model organisms, more tools will be required that function robustly in a wide range of bacterial hosts. The pGinger family of plasmids constitutes 43 plasmids that will enable both constitutive and inducible gene expression in a wide range of nonmodel Proteobacteria .

• Sponsoring Organization: USDOE
• Grant/Contract Number: AC02-05CH11231
• OSTI ID: 1974645
• Alternate ID(s): OSTI ID: 2228864
• Journal Information: Microbiology Spectrum, Journal Name: Microbiology Spectrum Journal Issue: 3 Vol. 11; ISSN 2165-0497
• Publisher: American Society for MicrobiologyCopyright Statement
• Country of Publication: United States
• Language: English