Whole‐Cell MALDI‐ToF MS Coupled with Untargeted Metabolomics Facilitates Investigations of Microbial Chemical Interactions

Aiosa, Nicole; Sinha, Anupama; Albataineh, Hanan; Phillips, Ashlee M.; Mageeney, Catherine M.; Wilde, Delaney S.; Williams, Kelly P.; Collette, Nicole M.; Branda, Steven S.; Garg, Neha

doi:10.1002/cbic.202200802

Title: Whole‐Cell MALDI‐ToF MS Coupled with Untargeted Metabolomics Facilitates Investigations of Microbial Chemical Interactions

Journal Article · 09 March 2023 · ChemBioChem: a European journal of chemical biology

DOI: https://doi.org/10.1002/cbic.202200802 · OSTI ID:1968096

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School of Chemistry and Biochemistry Georgia Institute of Technology 950 Atlantic Drive Atlanta GA 30332 USA
Biotechnology &, Bioengineering Sandia National Laboratories 7011 East Avenue Livermore CA 94550 USA
Biosciences and Biotechnology Division Lawrence Livermore National Laboratory 7000 East Avenue Livermore CA 94550 USA
Department of Bioengineering University of Pennsylvania 210 South 33rd Street Philadelphia PA 19104 USA
Systems Biology Sandia National Laboratories 7011 East Avenue Livermore CA 94550 USA
School of Chemistry and Biochemistry Georgia Institute of Technology 950 Atlantic Drive Atlanta GA 30332 USA, Center for Microbial Dynamics and Infection Georgia Institute of Technology 311 Ferst Drive, ES&,T Atlanta GA 30332 USA

Abstract The emergence of drug‐resistant pathogens necessitates the development of new countermeasures. In this regard, the introduction of probiotics to directly attack or competitively exclude pathogens presents a useful strategy. Application of this approach requires an understanding of how a probiotic and its target pathogen interact. A key means of probiotic‐pathogen interaction involves the production of small molecules called natural products (NPs). Here, we report the use of whole‐cell matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐ToF) mass spectrometry to characterize NP production by candidate probiotics (mouse airway microbiome isolates) when co‐cultured with the respiratory pathogen Burkholderia . We found that a Bacillus velezensis strain inhibits growth of and elicits NP production by Burkholderia thailandensis . Dereplication of known NPs detected in the metabolome of this B. velezensis strain suggests that a previously unannotated bioactive compound is involved. Thus, we present the use of whole‐cell MALDI as a broadly applicable method for screening the NP composition of microbial co‐cultures; this can be combined with other ‐omics methods to characterize probiotic‐pathogen and other microbe‐microbe interactions.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1968096

Journal Information:: ChemBioChem: a European journal of chemical biology, Journal Name: ChemBioChem: a European journal of chemical biology Journal Issue: 7 Vol. 24; ISSN 1439-4227

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: France

Language:: English

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