Rapid screening of secondary aromatic metabolites in Populus trichocarpa leaves

Harman-Ware, Anne E.; Martin, Madhavi Z.; Engle, Nancy L.; Doeppke, Crissa; Tschaplinski, Timothy J.

doi:10.1186/s13068-023-02287-2

Title: Rapid screening of secondary aromatic metabolites in Populus trichocarpa leaves

Journal Article · 10 March 2023 · Biotechnology for Biofuels and Bioproducts

DOI: https://doi.org/10.1186/s13068-023-02287-2 · OSTI ID:1963290

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National Renewable Energy Lab. (NREL), Golden, CO (United States). Renewable Resources and Enabling Science Center
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation (CBI)

High-throughput metabolomics analytical methodology is needed for population-scale studies of bioenergy-relevant feedstocks such as poplar (Populus sp.). Here, the authors report the relative abundance of extractable aromatic metabolites in Populus trichocarpa leaves rapidly estimated using pyrolysis-molecular beam mass spectrometry (py-MBMS). Poplar leaves were analyzed in conjunction with and validated by GC/MS analysis of extracts to determine key spectral features used to build PLS models to predict the relative composition of extractable aromatic metabolites in whole poplar leaves.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Energy Efficiency and Renewable Energy (EERE); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: AC05-00OR22725; Center for Bioenergy Innovation; AC36-08GO28308

OSTI ID:: 1963290

Alternate ID(s):: OSTI ID: 1965264; OSTI ID: 1969266

Report Number(s):: NREL/JA-2800-85882

Journal Information:: Biotechnology for Biofuels and Bioproducts, Vol. 16, Issue 1; ISSN 2731-3654

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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