Activity‐Based Protein Profiling – Enabling Phenotyping of Host‐Associated and Environmental Microbiomes

Wright, Aaron T.; Hudson, LaRae A.; Garcia, Whitney L.

doi:10.1002/ijch.202200099

Title: Activity‐Based Protein Profiling – Enabling Phenotyping of Host‐Associated and Environmental Microbiomes

Journal Article · 03 January 2023 · Israel Journal of Chemistry

DOI: https://doi.org/10.1002/ijch.202200099 · OSTI ID:1907917

^[1]; Hudson, LaRae A. ^[2]; Garcia, Whitney L. ^[2]

Department of Biology Baylor University Waco Texas 76798 USA, Department of Chemistry and Biochemistry Baylor University Waco Texas 76798 USA
Department of Biology Baylor University Waco Texas 76798 USA

Abstract Host‐associated and environmental microbiomes play central roles in human health, biogeochemical cycling, response to ecosystem change, and agriculture. Scientific approaches that can reveal the functional activities that contribute to observed phenotypes are needed in microbiome research. Broad characterization of the functional activity of microbes within microbiomes is currently hampered by approaches that rely on inference of function from metagenomes or indirect measurements. Activity‐based protein profiling is uniquely positioned to overcome these challenges and reveal the protein‐level mechanisms associated with microbiome phenotypes. In this review we describe the progress made to date using ABPP in gut microbiome, plant‐microbe interaction, and soil microbiome research, and suggest how ABPP data can be coupled with advanced computational methods to enable phenotype prediction and bioengineering of microbiomes for applied purposes.

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Research Organization:: Univ. of Delaware, Newark, DE (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: SC0022018

OSTI ID:: 1907917

Journal Information:: Israel Journal of Chemistry, Journal Name: Israel Journal of Chemistry Journal Issue: 3-4 Vol. 63; ISSN 0021-2148

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Israel

Language:: English

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