The development and use of metal-based probes for X-ray fluorescence microscopy

Victor-Lovelace, Tiffany W.; Miller, Lisa M.

doi:10.1093/mtomcs/mfac093

Title: The development and use of metal-based probes for X-ray fluorescence microscopy

Journal Article · 28 November 2022 · Metallomics (Online)

DOI: https://doi.org/10.1093/mtomcs/mfac093 · OSTI ID:1905940

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Abstract X-ray fluorescence microscopy (XFM) has become a widely used technique for imaging the concentration and distribution of metal ions in cells and tissues. Recent advances in synchrotron sources, optics, and detectors have improved the spatial resolution of the technique to <10 nm with attogram detection sensitivity. However, to make XFM most beneficial for bioimaging—especially at the nanoscale—the metal ion distribution must be visualized within the subcellular context of the cell. Over the years, a number of approaches have been taken to develop X-ray-sensitive tags that permit the visualization of specific organelles or proteins using XFM. In this review, we examine the types of X-ray fluorophore used, including nanomaterials and metal ions, and the approaches used to incorporate the metal into their target binding site via antibodies, genetically encoded metal-binding peptides, affinity labeling, or cell-specific peptides. We evaluate their advantages and disadvantages, review the scientific findings, and discuss the needs for future development.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0012704

OSTI ID:: 1905940

Report Number(s):: BNL-223904-2023-JAAM; mfac093

Journal Information:: Metallomics (Online), Journal Name: Metallomics (Online) Journal Issue: 12 Vol. 14; ISSN 1756-591X

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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