Title: Evaluation of an ASFV RNA Helicase Gene A859L for Virus Replication and Swine Virulence

Journal Article · · Viruses
DOI: https://doi.org/10.3390/v14010010 · OSTI ID:1905060
 [1];  [2];  [3];  [4];  [3];  [3]; ORCiD logo [3];  [3]
  1. US Dept. of Agriculture (USDA), Orient, NY (United States). Agricultural Research Service; OSTI
  2. US Dept. of Agriculture (USDA), Orient, NY (United States). Agricultural Research Service; Mississippi State Univ., Starkville, MS (United States)
  3. US Dept. of Agriculture (USDA), Orient, NY (United States). Agricultural Research Service
  4. US Dept. of Agriculture (USDA), Orient, NY (United States). Agricultural Research Service; Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
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African swine fever virus (ASFV) is producing a devastating pandemic that, since 2007, has spread to a contiguous geographical area from central Europe to Asia. In July 2021, ASFV was detected in the Dominican Republic, the first report of the disease in the Americas in more than 40 years. ASFV is a large, highly complex virus harboring a large dsDNA genome that encodes for more than 150 genes. The majority of these genes have not been functionally characterized. Bioinformatics analysis predicts that ASFV gene A859L encodes for an RNA helicase, although its function has not yet been experimentally assessed. Here, we evaluated the role of the A859L gene during virus replication in cell cultures and during infection in swine. For that purpose, a recombinant virus (ASFV-G-ΔA859L) harboring a deletion of the A859L gene was developed using the highly virulent ASFV Georgia (ASFV-G) isolate as a template. Recombinant ASFV-G-ΔA859L replicates in swine macrophage cultures as efficiently as the parental virus ASFV-G, demonstrating that the A859L gene is non-essential for ASFV replication. Experimental infection of domestic pigs demonstrated that ASFV-G-ΔA859L replicates as efficiently and induces a clinical disease indistinguishable from that caused by the parental ASFV-G. These studies conclude that the predicted RNA helicase gene A859L is not involved in the processes of virus replication or disease production in swine.

Research Organization:
Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
Sponsoring Organization:
US Dept. of Homeland Security (DHS); USDOE Office of Science (SC)
Grant/Contract Number:
SC0014664
OSTI ID:
1905060
Journal Information:
Viruses, Journal Name: Viruses Journal Issue: 1 Vol. 14; ISSN 1999-4915
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English

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59 BASIC BIOLOGICAL SCIENCES
A859L
ASF
ASFV
African swine fever virus
helicase
virology