Deletion of the EP296R Gene from the Genome of Highly Virulent African Swine Fever Virus Georgia 2010 Does Not Affect Virus Replication or Virulence in Domestic Pigs
Abstract
African swine fever virus (ASFV) causes a lethal disease (ASF) in domestic pigs, African swine fever (ASF). ASF is currently producing a pandemic affecting pig production across Eurasia, leading to a shortage of food accessibility. ASFV is structurally complex, harboring a large genome encoding over 150 genes. One of them, EP296R, has been shown to encode for an endonuclease that is necessary for the efficient replication of the virus in swine macrophages, the natural ASFV target cell. Here, we report the development of a recombinant virus, ASFV-G-ΔEP296R, harboring the deletion of the EP296R gene from the genome of the highly virulent field isolate ASFV Georgia 2010 (ASFV-G). The recombinant ASFV-G-ΔEP296R replicates in primary swine macrophages with similar kinetics as the parental virus ASFV-G. Pigs experimentally infected by the intramuscular route with 102 HAD50 show a slightly protracted, although lethal, presentation of the disease when compared to that of animals inoculated with parental ASFV-G. Viremia titers in the ASFV-G-ΔEP296R-infected animals closely followed the kinetics of presentation of clinical disease. Results presented here demonstrate that ASFV-G-ΔEP296R is not essential for the processes of ASFV replication in swine macrophages, nor is it radically involved in the process of virus replication or disease productionmore »
- Authors:
-
[2]
- US Dept. of Agriculture (USDA), Greenport, NY (United States); Mississippi State University, Starkville, MS (United States)
- US Dept. of Agriculture (USDA), Greenport, NY (United States)
- US Dept. of Agriculture (USDA), Greenport, NY (United States); Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); US Department of Homeland Security (DHS); Plum Island Animal Disease Center (PIADC); US Department of Agriculture (USDA)
- OSTI Identifier:
- 1904929
- Grant/Contract Number:
- SC0014664; 70RSAT19KPM000056
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Viruses
- Additional Journal Information:
- Journal Volume: 14; Journal Issue: 8; Journal ID: ISSN 1999-4915
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; ASFV; ASF; African swine fever; African swine fever virus; EP296R; virus virulence
Citation Formats
Vuono, Elizabeth A., Ramirez-Medina, Elizabeth, Pruitt, Sarah, Rai, Ayushi, Espinoza, Nallely, Spinard, Edward, Valladares, Alyssa, Silva, Ediane, Velazquez-Salinas, Lauro, Borca, Manuel V., and Gladue, Douglas P. Deletion of the EP296R Gene from the Genome of Highly Virulent African Swine Fever Virus Georgia 2010 Does Not Affect Virus Replication or Virulence in Domestic Pigs. United States: N. p., 2022.
Web. doi:10.3390/v14081682.
Vuono, Elizabeth A., Ramirez-Medina, Elizabeth, Pruitt, Sarah, Rai, Ayushi, Espinoza, Nallely, Spinard, Edward, Valladares, Alyssa, Silva, Ediane, Velazquez-Salinas, Lauro, Borca, Manuel V., & Gladue, Douglas P. Deletion of the EP296R Gene from the Genome of Highly Virulent African Swine Fever Virus Georgia 2010 Does Not Affect Virus Replication or Virulence in Domestic Pigs. United States. https://doi.org/10.3390/v14081682
Vuono, Elizabeth A., Ramirez-Medina, Elizabeth, Pruitt, Sarah, Rai, Ayushi, Espinoza, Nallely, Spinard, Edward, Valladares, Alyssa, Silva, Ediane, Velazquez-Salinas, Lauro, Borca, Manuel V., and Gladue, Douglas P. Sat .
"Deletion of the EP296R Gene from the Genome of Highly Virulent African Swine Fever Virus Georgia 2010 Does Not Affect Virus Replication or Virulence in Domestic Pigs". United States. https://doi.org/10.3390/v14081682. https://www.osti.gov/servlets/purl/1904929.
@article{osti_1904929,
title = {Deletion of the EP296R Gene from the Genome of Highly Virulent African Swine Fever Virus Georgia 2010 Does Not Affect Virus Replication or Virulence in Domestic Pigs},
author = {Vuono, Elizabeth A. and Ramirez-Medina, Elizabeth and Pruitt, Sarah and Rai, Ayushi and Espinoza, Nallely and Spinard, Edward and Valladares, Alyssa and Silva, Ediane and Velazquez-Salinas, Lauro and Borca, Manuel V. and Gladue, Douglas P.},
abstractNote = {African swine fever virus (ASFV) causes a lethal disease (ASF) in domestic pigs, African swine fever (ASF). ASF is currently producing a pandemic affecting pig production across Eurasia, leading to a shortage of food accessibility. ASFV is structurally complex, harboring a large genome encoding over 150 genes. One of them, EP296R, has been shown to encode for an endonuclease that is necessary for the efficient replication of the virus in swine macrophages, the natural ASFV target cell. Here, we report the development of a recombinant virus, ASFV-G-ΔEP296R, harboring the deletion of the EP296R gene from the genome of the highly virulent field isolate ASFV Georgia 2010 (ASFV-G). The recombinant ASFV-G-ΔEP296R replicates in primary swine macrophages with similar kinetics as the parental virus ASFV-G. Pigs experimentally infected by the intramuscular route with 102 HAD50 show a slightly protracted, although lethal, presentation of the disease when compared to that of animals inoculated with parental ASFV-G. Viremia titers in the ASFV-G-ΔEP296R-infected animals closely followed the kinetics of presentation of clinical disease. Results presented here demonstrate that ASFV-G-ΔEP296R is not essential for the processes of ASFV replication in swine macrophages, nor is it radically involved in the process of virus replication or disease production in domestic pigs.},
doi = {10.3390/v14081682},
journal = {Viruses},
number = 8,
volume = 14,
place = {United States},
year = {Sat Jul 30 00:00:00 EDT 2022},
month = {Sat Jul 30 00:00:00 EDT 2022}
}
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