Curcumin Nanodiscs Improve Solubility and Serve as Radiological Protectants against Ionizing Radiation Exposures in a Cell-Cycle Dependent Manner
Abstract
Curcumin, a natural polyphenol derived from the spice turmeric (Curcuma longa), contains antioxidant, anti-inflammatory, and anti-cancer properties. However, curcumin bioavailability is inherently low due to poor water solubility and rapid metabolism. Here, we further refined for use curcumin incorporated into “biomimetic” nanolipoprotein particles (cNLPs) consisting of a phospholipid bilayer surrounded by apolipoprotein A1 and amphipathic polymer scaffolding moieties. Our cNLP formulation improves the water solubility of curcumin over 30-fold and produces nanoparticles with ~350 µg/mL total loading capacity for downstream in vitro and in vivo applications. We found that cNLPs were well tolerated in AG05965/MRC-5 human primary lung fibroblasts compared to cultures treated with curcumin solubilized in DMSO (curDMSO). Pre-treatment with cNLPs of quiescent G0/G1-phase MRC-5 cultures improved cell survival following 137Cs gamma ray irradiations, although this finding was reversed in asynchronously cycling log-phase cell cultures. These findings may be useful for establishing cNLPs as a method to improve curcumin bioavailability for administration as a radioprotective and/or radiomitigative agent against ionizing radiation (IR) exposures in non-cycling cells or as a radiosensitizing agent for actively dividing cell populations, such as tumors.
- Authors:
-
[3];
[5];
[7];
[8];
[1]
- Univ. of California Davis, Sacramento, CA (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Univ. of California Davis, Sacramento, CA (United States); Univ. of London (United Kingdom)
- Helmholtz Zentrum München (Germany); Technical Univ. of Munich (Germany)
- Oncogenomic Consortium, Mexico City (Mexico)
- Wake Forest Univ., Winston-Salem, NC (United States)
- Colorado State Univ., Fort Collins, CO (United States)
- Univ. of California Davis, Sacramento, CA (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE Laboratory Directed Research and Development (LDRD) Program
- OSTI Identifier:
- 1900155
- Report Number(s):
- PNNL-SA-174987
Journal ID: ISSN 2079-4991
- Grant/Contract Number:
- AC05-76RL01830; AC52-07NA27344
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nanomaterials
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 20; Journal ID: ISSN 2079-4991
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; radiation; radioprotection; radiomitigation; DNA damage; countermeasure; nanoparticle; human cells; curcumin; nanodisc; ionizing radiation; radiosensitizer
Citation Formats
Evans, Angela C., Martin, Kelly A., Saxena, Manoj, Bicher, Sandra, Wheeler, Elizabeth, Cordova, Emilio J., Porada, Christopher D., Almeida-Porada, Graça, Kato, Takamitsu A., Wilson, Paul F., and Coleman, Matthew A. Curcumin Nanodiscs Improve Solubility and Serve as Radiological Protectants against Ionizing Radiation Exposures in a Cell-Cycle Dependent Manner. United States: N. p., 2022.
Web. doi:10.3390/nano12203619.
Evans, Angela C., Martin, Kelly A., Saxena, Manoj, Bicher, Sandra, Wheeler, Elizabeth, Cordova, Emilio J., Porada, Christopher D., Almeida-Porada, Graça, Kato, Takamitsu A., Wilson, Paul F., & Coleman, Matthew A. Curcumin Nanodiscs Improve Solubility and Serve as Radiological Protectants against Ionizing Radiation Exposures in a Cell-Cycle Dependent Manner. United States. https://doi.org/10.3390/nano12203619
Evans, Angela C., Martin, Kelly A., Saxena, Manoj, Bicher, Sandra, Wheeler, Elizabeth, Cordova, Emilio J., Porada, Christopher D., Almeida-Porada, Graça, Kato, Takamitsu A., Wilson, Paul F., and Coleman, Matthew A. Sat .
"Curcumin Nanodiscs Improve Solubility and Serve as Radiological Protectants against Ionizing Radiation Exposures in a Cell-Cycle Dependent Manner". United States. https://doi.org/10.3390/nano12203619. https://www.osti.gov/servlets/purl/1900155.
@article{osti_1900155,
title = {Curcumin Nanodiscs Improve Solubility and Serve as Radiological Protectants against Ionizing Radiation Exposures in a Cell-Cycle Dependent Manner},
author = {Evans, Angela C. and Martin, Kelly A. and Saxena, Manoj and Bicher, Sandra and Wheeler, Elizabeth and Cordova, Emilio J. and Porada, Christopher D. and Almeida-Porada, Graça and Kato, Takamitsu A. and Wilson, Paul F. and Coleman, Matthew A.},
abstractNote = {Curcumin, a natural polyphenol derived from the spice turmeric (Curcuma longa), contains antioxidant, anti-inflammatory, and anti-cancer properties. However, curcumin bioavailability is inherently low due to poor water solubility and rapid metabolism. Here, we further refined for use curcumin incorporated into “biomimetic” nanolipoprotein particles (cNLPs) consisting of a phospholipid bilayer surrounded by apolipoprotein A1 and amphipathic polymer scaffolding moieties. Our cNLP formulation improves the water solubility of curcumin over 30-fold and produces nanoparticles with ~350 µg/mL total loading capacity for downstream in vitro and in vivo applications. We found that cNLPs were well tolerated in AG05965/MRC-5 human primary lung fibroblasts compared to cultures treated with curcumin solubilized in DMSO (curDMSO). Pre-treatment with cNLPs of quiescent G0/G1-phase MRC-5 cultures improved cell survival following 137Cs gamma ray irradiations, although this finding was reversed in asynchronously cycling log-phase cell cultures. These findings may be useful for establishing cNLPs as a method to improve curcumin bioavailability for administration as a radioprotective and/or radiomitigative agent against ionizing radiation (IR) exposures in non-cycling cells or as a radiosensitizing agent for actively dividing cell populations, such as tumors.},
doi = {10.3390/nano12203619},
journal = {Nanomaterials},
number = 20,
volume = 12,
place = {United States},
year = {Sat Oct 15 00:00:00 EDT 2022},
month = {Sat Oct 15 00:00:00 EDT 2022}
}
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