Structural transitions in the GTP cap visualized by cryo-electron microscopy of catalytically inactive microtubules

LaFrance, Benjamin J.; Roostalu, Johanna; Henkin, Gil; Greber, Basil J.; Zhang, Rui; Normanno, Davide; McCollum, Chloe O.; Surrey, Thomas; Nogales, Eva

doi:10.1073/pnas.2114994119

Title: Structural transitions in the GTP cap visualized by cryo-electron microscopy of catalytically inactive microtubules

Journal Article · 07 January 2022 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.2114994119 · OSTI ID:1897632

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Univ. of California, Berkeley, CA (United States)
The Francis Crick Institute, London (United Kingdom)
The Francis Crick Institute, London (United Kingdom); Barcelona Institute of Science and Technology (BIST) (Spain)
Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Barcelona Institute of Science and Technology (BIST) (Spain)
The Francis Crick Institute, London (United Kingdom); Barcelona Institute of Science and Technology (BIST) (Spain); Catalan Institution for Research and Advanced Studies (ICREA) (Spain)

Microtubules (MTs) are nonequilibrium polymers that switch between states of growth and shrinkage. This property is critical for their function and is a consequence of GTP hydrolysis in the MT. The structure of the stable GTP part of the MT (the GTP cap) has previously been inferred from MTs polymerized with nonhydrolyzable GTP analogs. Here, we report high-resolution structures of MTs truly containing GTP, polymerized from mutated, hydrolysis-deficient tubulins. We find that GTP-MTs have an “expanded lattice” and a “closed seam,” structural characteristics possibly responsible for stabilizing the GTP cap. These results provide insight into the structural transitions at growing MT ends, furthering our understanding of the bistable nature of MTs.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: Cancer Research UK; European Research Council (ERC); National Institutes of Health (NIH); National Science Foundation (NSF); UK Medical Research Council; USDOE Office of Science (SC), Basic Energy Sciences (BES); Wellcome Trust

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1897632

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 2 Vol. 119; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Structural transitions in the GTP cap visualized by cryo-electron microscopy of catalytically inactive microtubules

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