Metabolic engineering of p–hydroxybenzoate in poplar lignin

Mottiar, Yaseen; Karlen, Steven D.; Goacher, Robyn E.; Ralph, John; Mansfield, Shawn D.

doi:10.1111/pbi.13935

Title: Metabolic engineering of p–hydroxybenzoate in poplar lignin

Journal Article · 26 September 2022 · Plant Biotechnology Journal

DOI: https://doi.org/10.1111/pbi.13935 · OSTI ID:1893926

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Univ. of British Columbia, Vancouver, BC (Canada); Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); University of British Columbia Department of Wood Science
Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
Niagara University, North Tonawanda, NY (United States)
Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); Univ. of Wisconsin, Madison, WI (United States)
Univ. of British Columbia, Vancouver, BC (Canada); Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Ester-linked p-hydroxybenzoate occurs naturally in poplar lignin as pendent groups that can be released by mild alkaline hydrolysis. These ‘clip-off’ phenolics can be separated from biomass and upgraded into diverse high-value bioproducts. We introduced a bacterial chorismate pyruvate lyase gene into transgenic poplar trees with the aim of producing more p-hydroxybenzoate from chorismate, itself a metabolic precursor to lignin. By driving heterologous expression specifically in the plastids of cells undergoing secondary wall formation, this strategy achieved a 50% increase in cell-wall-bound p-hydroxybenzoate in mature wood and nearly 10 times more in developing xylem relative to control trees. Comparable amounts also remained as soluble p-hydroxybenzoate-containing xylem metabolites, pointing to even greater engineering potential. Mass spectrometry imaging showed that the elevated p-hydroxybenzoylation was largely restricted to the cell walls of fibres. Finally, transgenic lines outperformed control trees in assays of saccharification potential. Here, this study highlights the biotech potential of cell-wall-bound phenolate esters and demonstrates the importance of substrate supply in lignin engineering.

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Research Organization:: Great Lakes Bioenergy Research Center, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018409

OSTI ID:: 1893926

Journal Information:: Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal Journal Issue: 1 Vol. 21; ISSN 1467-7644

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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