Isolation and characterization of an anti-proliferative polysaccharide from the North American fungus Echinodontium tinctorium

Zeb, Mehreen; Li, Wai Ming; Heiss, Christian; Black, Ian; Tackaberry, Linda E.; Massicotte, Hugues B.; Egger, Keith N.; Reimer, Kerry; Azadi, Parastoo; Lee, Chow H.

doi:10.1038/s41598-022-21697-0

Title: Isolation and characterization of an anti-proliferative polysaccharide from the North American fungus Echinodontium tinctorium

Journal Article · 13 October 2022 · Scientific Reports

DOI: https://doi.org/10.1038/s41598-022-21697-0 · OSTI ID:1893179

Zeb, Mehreen; Li, Wai Ming; Heiss, Christian; Black, Ian; Tackaberry, Linda E.; Massicotte, Hugues B.; Egger, Keith N.; Reimer, Kerry; Azadi, Parastoo; Lee, Chow H.

Abstract A novel polysaccharide EtGIPL1a was purified from fruiting bodies of Echinodontium tinctorium , a fungus unique to western North America. EtGIPL1a has an estimated weight average molecular weight of 275 kDa and is composed of glucose (54.3%), galactose (19.6%), mannose (11.1%), fucose (10.3%), glucuronic acid (4%), and rhamnose (0.6%). It has multiple glycosidic linkages, with 3-Glc p (28.9%), 6-Glc p (18.3%), 3,6-Glc p (13%), 4-Glc p A (9.2%), 6-Gal p (3.9%), 2,6-Gal p (2.6%), 3-Fuc p (2.5%), 6-Man p (2.4%) being the most prominent, and unsubstituted glucose (15.3%), mannose (1.3%) and fucose (0.9%) as major terminal sugars. EtGIPL1a has a backbone containing mostly 3-substituted β -glucopyranose with 4-substituted glucopyranosyluronic acid. EtGIPL1a showed anti-proliferative activity against multiple cancer cell lines, with IC ₅₀ ranging from 50.6 to 1446 nM. Flow cytometry analyses confirmed that apoptosis induction is one mechanism for its anti-proliferative activity. EtGIPL1a should be further investigated for its potential anti-cancer activity in animal models, and for its possible utility in differentiation cancer therapy.

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Research Organization:: Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: SC0015662

OSTI ID:: 1893179

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 12; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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