Transcriptome and metabolome integration in sugarcane through culm development

Perlo, Virginie; Furtado, Agnelo; Botha, Frederik C.; Margarido, Gabriel R. A.; Hodgson‐Kratky, Katrina; Choudhary, Hemant; Gladden, John; Simmons, Blake; Henry, Robert J.

doi:10.1002/fes3.421

Title: Transcriptome and metabolome integration in sugarcane through culm development

Journal Article · 14 September 2022 · Food and Energy Security

DOI: https://doi.org/10.1002/fes3.421 · OSTI ID:1887613

Perlo, Virginie ^[1]; Furtado, Agnelo ^[1]; Botha, Frederik C. ^[1]; Margarido, Gabriel R. A. ^[2]; Hodgson‐Kratky, Katrina ^[1];

^[3]; Gladden, John ^[3]; Simmons, Blake ^[4];

^[1]

Queensland Alliance for Agriculture and Food Innovation University of Queensland Brisbane Australia
Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz” Universidade de São Paulo São Paulo Brazil
Joint BioEnergy Institute Emeryville CA USA, Sandia National Laboratories Livermore CA USA
Joint BioEnergy Institute Emeryville CA USA

Abstract Sugarcane (Saccharum sp.) is a tropical and subtropical C4 plant with a high photosynthetic and carbon assimilation efficiency that stores sucrose. Culm biomass is also composed of bagasse fibre, a by‐product of the sugarcane industry. This high‐yielding grass, high in sucrose and lignocellulosic biomass, is considered an optimal feedstock as an alternative to fossil fuels and to produce a broad range of high‐value biomaterials. The ideal sugarcane production system would optimise the relative production of sugar and these new products. Multi‐omics correlation analysis was used to generate a global view of the essential metabolic pathways identifying critical genes involved in carbon partitioning during different stages of development. This research employed an unprecedented metabolic and transcriptomic dataset of 360 samples from a selection of 1440 culms of 24 genotypes at five different development stages. Chemical composition and metabolome analysis showed an increase through the culm development of lignin, sucrose, carbon, and amino acids such as aspartic acid, serine, alanine, methionine, threonine 3‐cyano‐L‐alanine, and citric acid. Transcriptome analysis revealed functionalities such as transcription, nucleotide transport and metabolism, and the biosynthesis of amino acids that are highly activated during the immature stage and highly down‐regulated during the most mature age.

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Sponsoring Organization:: USDOE

OSTI ID:: 1887613

Journal Information:: Food and Energy Security, Journal Name: Food and Energy Security Journal Issue: 4 Vol. 11; ISSN 2048-3694

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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