Genome Sequence and Analysis of the Flavinogenic Yeast Candida membranifaciens IST 626

Palma, Margarida; Mondo, Stephen; Pereira, Mariana; Vieira, Érica; Grigoriev, Igor V.; Sá-Correia, Isabel

doi:10.3390/jof8030254

Title: Genome Sequence and Analysis of the Flavinogenic Yeast Candida membranifaciens IST 626

Journal Article · 28 February 2022 · Journal of Fungi

DOI: https://doi.org/10.3390/jof8030254 · OSTI ID:1885462

^[1]; Mondo, Stephen ^[2]; Pereira, Mariana ^[1]; Vieira, Érica ^[1]; Grigoriev, Igor V. ^[3];

^[1]

Inst. of Superior Tecnico (IST), Lisbon (Portugal)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Colorado State Univ., Fort Collins, CO (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)

The ascomycetous yeast Candida membranifaciens has been isolated from diverse habitats, including humans, insects, and environmental sources, exhibiting a remarkable ability to use different carbon sources that include pentoses, melibiose, and inulin. In this study, we isolated four C. membranifaciens strains from soil and investigated their potential to overproduce riboflavin. C. membranifaciens IST 626 was found to produce the highest concentrations of riboflavin. The volumetric production of this vitamin was higher when C. membranifaciens IST 626 cells were cultured in a commercial medium without iron and when xylose was the available carbon source compared to the same basal medium with glucose. Supplementation of the growth medium with 2 g/L glycine favored the metabolization of xylose, leading to biomass increase and consequent enhancement of riboflavin volumetric production that reached 120 mg/L after 216 h of cultivation. To gain new insights into the molecular basis of riboflavin production and carbon source utilization in this species, the first annotated genome sequence of C. membranifaciens is reported in this article, as well as the result of a comparative genomic analysis with other relevant yeast species. A total of 5619 genes were predicted to be present in C. membranifaciens IST 626 genome sequence (11.5 Mbp). Among them are genes involved in riboflavin biosynthesis, iron homeostasis, and sugar uptake and metabolism. This work put forward C. membranifaciens IST 626 as a riboflavin overproducer and provides valuable molecular data for future development of superior producing strains capable of using the wide range of carbon sources, which is a characteristic trait of the species.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: Fundação para a Ciência e a Tecnologia (FCT); Spanish Federation of Rare Diseases (FEDER); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1885462

Journal Information:: Journal of Fungi, Journal Name: Journal of Fungi Journal Issue: 3 Vol. 8; ISSN 2309-608X

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Candida membranifaciens
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iron metabolism
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xylose assimilation
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Title: Genome Sequence and Analysis of the Flavinogenic Yeast Candida membranifaciens IST 626

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