Detailed analysis of the D-galactose catabolic pathways in Aspergillus niger reveals complexity at both metabolic and regulatory level

Chroumpi, Tania; Martínez-Reyes, Natalia; Kun, Roland S.; Peng, Mao; Lipzen, Anna; Ng, Vivian; Tejomurthula, Sravanthi; Zhang, Yu; Grigoriev, Igor V.; Mäkelä, Miia R.; de Vries, Ronald P.; Garrigues, Sandra

doi:10.1016/j.fgb.2022.103670

Title: Detailed analysis of the D-galactose catabolic pathways in Aspergillus niger reveals complexity at both metabolic and regulatory level

Journal Article · 02 February 2022 · Fungal Genetics and Biology

DOI: https://doi.org/10.1016/j.fgb.2022.103670 · OSTI ID:1883339

Chroumpi, Tania ^[1]; Martínez-Reyes, Natalia ^[1]; Kun, Roland S. ^[1]; Peng, Mao ^[1]; Lipzen, Anna ^[2]; Ng, Vivian ^[2]; Tejomurthula, Sravanthi ^[2]; Zhang, Yu ^[2]; Grigoriev, Igor V. ^[3]; Mäkelä, Miia R. ^[4]; de Vries, Ronald P. ^[1]; Garrigues, Sandra ^[1]

Utrecht University (Netherlands)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
Univ. of Helsinki (Finland)

The current impetus towards a sustainable bio-based economy has accelerated research to better understand the mechanisms through which filamentous fungi convert plant biomass, a valuable feedstock for biotechnological applications. Several transcription factors have been reported to control the polysaccharide degradation and metabolism of the resulting sugars in fungi. However, little is known about their individual contributions, interactions and crosstalk. D-galactose is a hexose sugar present mainly in hemicellulose and pectin in plant biomass. Here, we study D-galactose conversion by Aspergillus niger and describe the involvement of the arabinanolytic and xylanolytic activators AraR and XlnR, in addition to the D-galactose-responsive regulator GalX. Our results deepen the understanding of the complexity of the filamentous fungal regulatory network for plant biomass degradation and sugar catabolism, and facilitate the generation of more efficient plant biomass-degrading strains for biotechnological applications.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); The Dutch Research Council (NWO); Ministry of Infrastructure and Water Management

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1883339

Journal Information:: Fungal Genetics and Biology, Journal Name: Fungal Genetics and Biology Vol. 159; ISSN 1087-1845

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Title: Detailed analysis of the D-galactose catabolic pathways in Aspergillus niger reveals complexity at both metabolic and regulatory level

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