De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Tetreau, Guillaume; Sawaya, Michael R.; De Zitter, Elke; Andreeva, Elena A.; Banneville, Anne-Sophie; Schibrowsky, Natalie A.; Coquelle, Nicolas; Brewster, Aaron S.; Grünbein, Marie Luise; Kovacs, Gabriela Nass; Hunter, Mark S.; Kloos, Marco; Sierra, Raymond G.; Schiro, Giorgio; Qiao, Pei; Stricker, Myriam; Bideshi, Dennis; Young, Iris D.; Zala, Ninon; Engilberge, Sylvain; Gorel, Alexander; Signor, Luca; Teulon, Jean-Marie; Hilpert, Mario; Foucar, Lutz; Bielecki, Johan; Bean, Richard; de Wijn, Raphael; Sato, Tokushi; Kirkwood, Henry; Letrun, Romain; Batyuk, Alexander; Snigireva, Irina; Fenel, Daphna; Schubert, Robin; Canfield, Ethan J.; Alba, Mario M.; Laporte, Frédéric; Després, Laurence; Bacia, Maria; Roux, Amandine; Chapelle, Christian; Riobé, François; Maury, Olivier; Ling, Wai Li; Boutet, Sébastien; Mancuso, Adrian; Gutsche, Irina; Girard, Eric; Barends, Thomas R. M.; Pellequer, Jean-Luc; Park, Hyun-Woo; Laganowsky, Arthur D.; Rodriguez, Jose; Burghammer, Manfred; Shoeman, Robert L.; Doak, R. Bruce; Weik, Martin; Sauter, Nicholas K.; Federici, Brian; Cascio, Duilio; Schlichting, Ilme; Colletier, Jacques-Philippe

doi:10.1038/s41467-022-31746-x

Title: De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Journal Article · 28 July 2022 · Nature Communications

DOI: https://doi.org/10.1038/s41467-022-31746-x · OSTI ID:1878424

Tetreau, Guillaume;

;

; Schibrowsky, Natalie A.; Coquelle, Nicolas;

; Grünbein, Marie Luise; Kovacs, Gabriela Nass;

; Kloos, Marco;

; Schiro, Giorgio; Qiao, Pei;

; Bideshi, Dennis;

; Zala, Ninon; Engilberge, Sylvain more »

Abstract Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan , respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.

View Journal Article

Cite

Export

Save

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: Agence Nationale de la Recherche; Centers for Disease Control and Prevention; Ecoles Universitaires de Recherche; National Institute of Health; USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1878424

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 13; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

References (91)

The HMMER Web Server for Protein Sequence Similarity Search Prakash, Ananth; Jeffryes, Matt; Bateman, Alex Current Protocols in Bioinformatics, Vol. 60, Issue 1 https://doi.org/10.1002/cpbi.40	journal	December 2017
Construction and Characterization of a Recombinant Bacillus thuringiensis subsp. israelensis Strain That Produces Cry11B Park, Hyun-Woo; Delécluse, Armelle; Federici, Brian A. Journal of Invertebrate Pathology, Vol. 78, Issue 1 https://doi.org/10.1006/jipa.2001.5038	journal	July 2001
Bacillus thuringiensisCrylA(a) Insecticidal Toxin: Crystal Structure and Channel Formation Grochulski, Pawel; Masson, Luke; Borisova, Svetlana Journal of Molecular Biology, Vol. 254, Issue 3 https://doi.org/10.1006/jmbi.1995.0630	journal	December 1995
Improved production of the insecticidal CryIVD protein in Bacillus thuringiensis using cryIA(c) promoters to express the gene for an associated 20-kDa protein Wu, D.; Federici, B. A. Applied Microbiology and Biotechnology, Vol. 42, Issue 5 https://doi.org/10.1007/BF00171947	journal	January 1995
Construction and characterization of the interdomain chimeras using Cry11Aa and Cry11Ba from Bacillus thuringiensis and identification of a possible novel toxic chimera Sun, Yunjun; Zhao, Qiang; Zheng, Dasheng Biotechnology Letters, Vol. 36, Issue 1 https://doi.org/10.1007/s10529-013-1330-3	journal	September 2013
In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis Rosenfeld, Jorge; Capdevielle, Joël; Guillemot, Jean Claude Analytical Biochemistry, Vol. 203, Issue 1 https://doi.org/10.1016/0003-2697(92)90061-B	journal	May 1992
Statistics of local complexity in amino acid sequences and sequence databases Wootton, John C.; Federhen, Scott Computers & Chemistry, Vol. 17, Issue 2, p. 149-163 https://doi.org/10.1016/0097-8485(93)85006-X	journal	June 1993
Rosetta3: An Object-Oriented Software Suite for the Simulation and Design of Macromolecules Leaver-Fay, Andrew; Tyka, Michael; Lewis, Steven M. Computer Methods, Part C https://doi.org/10.1016/B978-0-12-381270-4.00019-6	book	January 2011
Structure of Cry2Aa Suggests an Unexpected Receptor Binding Epitope Morse, R. J.; Yamamoto, T.; Stroud, R. M. Structure, Vol. 9, Issue 5 https://doi.org/10.1016/S0969-2126(01)00601-3	journal	May 2001
CASS—CFEL-ASG software suite Foucar, Lutz; Barty, Anton; Coppola, Nicola Computer Physics Communications, Vol. 183, Issue 10 https://doi.org/10.1016/j.cpc.2012.04.023	journal	October 2012
Cry11Aa toxin from Bacillus thuringiensis binds its receptor in Aedes aegypti mosquito larvae through loop α-8 of domain II Fernández, Luisa E.; Pérez, Claudia; Segovia, Lorenzo FEBS Letters, Vol. 579, Issue 17 https://doi.org/10.1016/j.febslet.2005.05.032	journal	June 2005
Loop residues of the receptor binding domain of Bacillus thuringiensis Cry11Ba toxin are important for mosquitocidal activity Likitvivatanavong, Supaporn; Aimanova, Karlygash G.; Gill, Sarjeet S. FEBS Letters, Vol. 583, Issue 12 https://doi.org/10.1016/j.febslet.2009.05.020	journal	May 2009
Current models of the mode of action of Bacillus thuringiensis insecticidal crystal proteins: A critical review Vachon, Vincent; Laprade, Raynald; Schwartz, Jean-Louis Journal of Invertebrate Pathology, Vol. 111, Issue 1 https://doi.org/10.1016/j.jip.2012.05.001	journal	September 2012
Contributions of 5′-UTR and 3′-UTR cis elements to Cyt1Aa synthesis in Bacillus thuringiensis subsp. israelensis Sakano, Yuko; Park, Hyun-Woo; Bideshi, Dennis K. Journal of Invertebrate Pathology, Vol. 149 https://doi.org/10.1016/j.jip.2017.08.002	journal	October 2017
Crystal Structure of the Mosquito-larvicidal Toxin Cry4Ba and Its Biological Implications Boonserm, Panadda; Davis, Paul; Ellar, David J. Journal of Molecular Biology, Vol. 348, Issue 2 https://doi.org/10.1016/j.jmb.2005.02.013	journal	April 2005
Inference of Macromolecular Assemblies from Crystalline State Krissinel, Evgeny; Henrick, Kim Journal of Molecular Biology, Vol. 372, Issue 3 https://doi.org/10.1016/j.jmb.2007.05.022	journal	September 2007
Cyt1Aa Toxin: Crystal Structure Reveals Implications for Its Membrane-Perforating Function Cohen, Shmuel; Albeck, Shira; Ben-Dov, Eitan Journal of Molecular Biology, Vol. 413, Issue 4 https://doi.org/10.1016/j.jmb.2011.09.021	journal	November 2011
The adaptive gain integrating pixel detector AGIPD a detector for the European XFEL Henrich, B.; Becker, J.; Dinapoli, R. Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 633 https://doi.org/10.1016/j.nima.2010.06.107	journal	May 2011
Bayesian Deconvolution of Mass and Ion Mobility Spectra: From Binary Interactions to Polydisperse Ensembles Marty, Michael T.; Baldwin, Andrew J.; Marklund, Erik G. Analytical Chemistry, Vol. 87, Issue 8 https://doi.org/10.1021/acs.analchem.5b00140	journal	April 2015
Multiple Receptors as Targets of Cry Toxins in Mosquitoes Likitvivatanavong, Supaporn; Chen, Jianwu; Evans, Amy M. Journal of Agricultural and Food Chemistry, Vol. 59, Issue 7 https://doi.org/10.1021/jf1036189	journal	April 2011
Crystal structure of insecticidal δ-endotoxin from Bacillus thuringiensis at 2.5 Å resolution Li, Jade; Carroll, Joe; Ellar, David J. Nature, Vol. 353, Issue 6347 https://doi.org/10.1038/353815a0	journal	October 1991
Femtosecond X-ray protein nanocrystallography Chapman, Henry N.; Fromme, Petra; Barty, Anton Nature, Vol. 470, Issue 7332, p. 73-77 https://doi.org/10.1038/nature09750	journal	February 2011
De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure Colletier, Jacques-Philippe; Sawaya, Michael R.; Gingery, Mari Nature, Vol. 539, Issue 7627 https://doi.org/10.1038/nature19825	journal	September 2016
MMseqs2 enables sensitive protein sequence searching for the analysis of massive data sets Steinegger, Martin; Söding, Johannes Nature Biotechnology, Vol. 35, Issue 11 https://doi.org/10.1038/nbt.3988	journal	October 2017
Automatic protein structure solution from weak X-ray data Skubák, Pavol; Pannu, Navraj S. Nature Communications, Vol. 4, Issue 1 https://doi.org/10.1038/ncomms3777	journal	November 2013
Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers Hattne, Johan; Echols, Nathaniel; Tran, Rosalie Nature Methods, Vol. 11, Issue 5 https://doi.org/10.1038/nmeth.2887	journal	March 2014
Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II Sierra, Raymond G.; Gati, Cornelius; Laksmono, Hartawan Nature Methods, Vol. 13, Issue 1 https://doi.org/10.1038/nmeth.3667	journal	November 2015
Mass spectrometry of intact membrane protein complexes Laganowsky, Arthur; Reading, Eamonn; Hopper, Jonathan T. S. Nature Protocols, Vol. 8, Issue 4 https://doi.org/10.1038/nprot.2013.024	journal	March 2013
Extreme amyloid polymorphism in Staphylococcus aureus virulent PSMα peptides Salinas, Nir; Colletier, Jacques-Philippe; Moshe, Asher Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-05490-0	journal	August 2018
Megahertz data collection from protein microcrystals at an X-ray free-electron laser Grünbein, Marie Luise; Bielecki, Johan; Gorel, Alexander Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-05953-4	journal	August 2018
Megahertz serial crystallography Wiedorn, Max O.; Oberthür, Dominik; Bean, Richard Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-06156-7	journal	October 2018
Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade Tetreau, Guillaume; Banneville, Anne-Sophie; Andreeva, Elena A. Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-14894-w	journal	March 2020
Highly accurate protein structure prediction with AlphaFold Jumper, John; Evans, Richard; Pritzel, Alexander Nature https://doi.org/10.1038/s41586-021-03819-2	journal	July 2021
Crystallophore: a versatile lanthanide complex for protein crystallography combining nucleating effects, phasing properties, and luminescence Engilberge, Sylvain; Riobé, François; Di Pietro, Sebastiano Chemical Science, Vol. 8, Issue 9 https://doi.org/10.1039/C7SC00758B	journal	January 2017
A GPI-anchored alkaline phosphatase is a functional midgut receptor of Cry11Aa toxin in Aedes aegypti larvae Fernandez, Luisa E.; Aimanova, Karlygash G.; Gill, Sarjeet S. Biochemical Journal, Vol. 394, Issue 1 https://doi.org/10.1042/BJ20051517	journal	January 2006
Biochemical characterization of Bacillus thuringiensis cytolytic toxins in association with a phospholipid bilayer Du, Jinping; Knowles, Barbara H.; Li, Jade Biochemical Journal, Vol. 338, Issue 1 https://doi.org/10.1042/bj3380185	journal	February 1999
Bacillus thuringiensis subsp. israelensis Cyt1Aa synergizes Cry11Aa toxin by functioning as a membrane-bound receptor Perez, C.; Fernandez, L. E.; Sun, J. Proceedings of the National Academy of Sciences, Vol. 102, Issue 51 https://doi.org/10.1073/pnas.0505494102	journal	December 2005
Protein crystal structure obtained at 2.9 A resolution from injecting bacterial cells into an X-ray free-electron laser beam Sawaya, M. R.; Cascio, D.; Gingery, M. Proceedings of the National Academy of Sciences, Vol. 111, Issue 35 https://doi.org/10.1073/pnas.1413456111	journal	August 2014
Gas dynamic virtual nozzle for generation of microscopic droplet streams DePonte, D. P.; Weierstall, U.; Schmidt, K. Journal of Physics D: Applied Physics, Vol. 41, Issue 19, Article No. 195505 https://doi.org/10.1088/0022-3727/41/19/195505	journal	September 2008
CAST: an iterative algorithm for the complexity analysis of sequence tracts Promponas, V. J.; Enright, A. J.; Tsoka, S. Bioinformatics, Vol. 16, Issue 10 https://doi.org/10.1093/bioinformatics/16.10.915	journal	October 2000
STRAP: editor for STRuctural Alignments of Proteins Gille, C.; Frommel, C. Bioinformatics, Vol. 17, Issue 4 https://doi.org/10.1093/bioinformatics/17.4.377	journal	April 2001
Fast and sensitive taxonomic assignment to metagenomic contigs Mirdita, M.; Steinegger, M.; Breitwieser, F. Bioinformatics, Vol. 37, Issue 18 https://doi.org/10.1093/bioinformatics/btab184	journal	March 2021
LCR-eXXXplorer: a web platform to search, visualize and share data for low complexity regions in protein sequences Kirmitzoglou, Ioannis; Promponas, Vasilis J. Bioinformatics, Vol. 31, Issue 13 https://doi.org/10.1093/bioinformatics/btv115	journal	February 2015
The SWISS-PROT protein sequence data bank and its supplement TrEMBL in 1999 Bairoch, A.; Apweiler, R. Nucleic Acids Research, Vol. 27, Issue 1 https://doi.org/10.1093/nar/27.1.49	journal	January 1999
Structural and functional characterization of DdrC, a novel DNA damage-induced nucleoid associated protein involved in DNA compaction Banneville, Anne-Sophie; Bouthier de la Tour, Claire; De Bonis, Salvatore Nucleic Acids Research, Vol. 50, Issue 13 https://doi.org/10.1093/nar/gkac563	journal	July 2022
T-REX: a web server for inferring, validating and visualizing phylogenetic trees and networks Boc, A.; Diallo, A. B.; Makarenkov, V. Nucleic Acids Research, Vol. 40, Issue W1 https://doi.org/10.1093/nar/gks485	journal	June 2012
Alignment-Annotator web server: rendering and annotating sequence alignments Gille, Christoph; Fähling, Michael; Weyand, Birgit Nucleic Acids Research, Vol. 42, Issue W1 https://doi.org/10.1093/nar/gku400	journal	May 2014
HMMER web server: 2018 update Potter, Simon C.; Luciani, Aurélien; Eddy, Sean R. Nucleic Acids Research, Vol. 46, Issue W1 https://doi.org/10.1093/nar/gky448	journal	June 2018
The EMBL-EBI search and sequence analysis tools APIs in 2019 Madeira, Fábio; Park, Young mi; Lee, Joon Nucleic Acids Research, Vol. 47, Issue W1 https://doi.org/10.1093/nar/gkz268	journal	April 2019
Low complexity regions in the proteins of prokaryotes perform important functional roles and are highly conserved Ntountoumi, Chrysa; Vlastaridis, Panayotis; Mossialos, Dimitris Nucleic Acids Research, Vol. 47, Issue 19 https://doi.org/10.1093/nar/gkz730	journal	September 2019
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
Indexing in single-crystal diffractometry with an obstinate list of reflections Duisenberg, A. J. M. Journal of Applied Crystallography, Vol. 25, Issue 2 https://doi.org/10.1107/S0021889891010634	journal	April 1992
MOLREP an Automated Program for Molecular Replacement Vagin, A.; Teplyakov, A. Journal of Applied Crystallography, Vol. 30, Issue 6, p. 1022-1025 https://doi.org/10.1107/S0021889897006766	journal	December 1997
Structure of the insecticidal bacterial δ-endotoxin Cry3Bb1 of Bacillus thuringiensis Galitsky, Nikolai; Cody, Vivian; Wojtczak, Andrzej Acta Crystallographica Section D Biological Crystallography, Vol. 57, Issue 8 https://doi.org/10.1107/S0907444901008186	journal	July 2001
Refinement of severely incomplete structures with maximum likelihood in BUSTER–TNT Blanc, E.; Roversi, P.; Vonrhein, C. Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12 https://doi.org/10.1107/S0907444904016427	journal	November 2004
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Secondary-structure matching (SSM), a new tool for fast protein structure alignment in three dimensions Krissinel, E.; Henrick, K. Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12 https://doi.org/10.1107/S0907444904026460	journal	November 2004
The integration of macromolecular diffraction data Leslie, Andrew G. W. Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 1 https://doi.org/10.1107/S0907444905039107	journal	December 2005
Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard Terwilliger, Thomas C.; Grosse-Kunstleve, Ralf W.; Afonine, Pavel V. Acta Crystallographica Section D Biological Crystallography, Vol. 64, Issue 1 https://doi.org/10.1107/S090744490705024X	journal	December 2007
ALINE : a WYSIWYG protein-sequence alignment editor for publication-quality alignments Bond, Charles Simon; Schüttelkopf, Alexander Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 65, Issue 5 https://doi.org/10.1107/S0907444909007835	journal	April 2009
Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910045749	journal	March 2011
REFMAC 5 for the refinement of macromolecular crystal structures Murshudov, Garib N.; Skubák, Pavol; Lebedev, Andrey A. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444911001314	journal	March 2011
Completion of autobuilt protein models using a database of protein fragments Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 4 https://doi.org/10.1107/S0907444911039655	journal	March 2012
Towards automated crystallographic structure refinement with phenix.refine Afonine, Pavel V.; Grosse-Kunstleve, Ralf W.; Echols, Nathaniel Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 4 https://doi.org/10.1107/S0907444912001308	journal	March 2012
New Python-based methods for data processing Sauter, Nicholas K.; Hattne, Johan; Grosse-Kunstleve, Ralf W. Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7 https://doi.org/10.1107/S0907444913000863	journal	June 2013
Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams Coquelle, Nicolas; Brewster, Aaron S.; Kapp, Ulrike Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 5 https://doi.org/10.1107/S1399004715004514	journal	April 2015
Protein crystal structure determination with the crystallophore, a nucleating and phasing agent Engilberge, Sylvain; Wagner, Tristan; Santoni, Gianluca Journal of Applied Crystallography, Vol. 52, Issue 4 https://doi.org/10.1107/S1600576719006381	journal	June 2019
The Coherent X-ray Imaging instrument at the Linac Coherent Light Source Liang, Mengning; Williams, Garth J.; Messerschmidt, Marc Journal of Synchrotron Radiation, Vol. 22, Issue 3 https://doi.org/10.1107/S160057751500449X	journal	April 2015
Serial femtosecond crystallography: the first five years Schlichting, Ilme IUCrJ, Vol. 2, Issue 2, p. 246-255 https://doi.org/10.1107/S205225251402702X	journal	February 2015
XGANDALF – extended gradient descent algorithm for lattice finding Gevorkov, Yaroslav; Yefanov, Oleksandr; Barty, Anton Acta Crystallographica Section A Foundations and Advances, Vol. 75, Issue 5 https://doi.org/10.1107/S2053273319010593	journal	August 2019
Diffraction-geometry refinement in the DIALS framework Waterman, David G.; Winter, Graeme; Gildea, Richard J. Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 4 https://doi.org/10.1107/S2059798316002187	journal	March 2016
TakeTwo: an indexing algorithm suited to still images with known crystal parameters Ginn, Helen Mary; Roedig, Philip; Kuo, Anling Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 8, p. 956-965 https://doi.org/10.1107/S2059798316010706	journal	July 2016
Distributed computing for macromolecular crystallography Krissinel, Evgeny; Uski, Ville; Lebedev, Andrey Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 2 https://doi.org/10.1107/S2059798317014565	journal	February 2018
DIALS : implementation and evaluation of a new integration package Winter, Graeme; Waterman, David G.; Parkhurst, James M. Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 2 https://doi.org/10.1107/S2059798317017235	journal	February 2018
Processing serial crystallography data with CrystFEL : a step-by-step guide White, Thomas A. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/S205979831801238X	journal	January 2019
Bacillus thuringiensis ssp. israelensis Cyt1Aa enhances activity of Cry11Aa toxin by facilitating the formation of a pre-pore oligomeric structure: Cyt1Aa facilitates oligomerization of Cry11Aa toxin Pérez, Claudia; Muñoz-Garay, Carlos; Portugal, Leivi C. Cellular Microbiology, Vol. 9, Issue 12 https://doi.org/10.1111/j.1462-5822.2007.01007.x	journal	August 2007
Bacillus thuringiensis insecticidal three-domain Cry toxins: mode of action, insect resistance and consequences for crop protection Pardo-López, Liliana; Soberón, Mario; Bravo, Alejandra FEMS Microbiology Reviews, Vol. 37, Issue 1 https://doi.org/10.1111/j.1574-6976.2012.00341.x	journal	January 2013
Atomic structures of low-complexity protein segments reveal kinked β sheets that assemble networks Hughes, Michael P.; Sawaya, Michael R.; Boyer, David R. Science, Vol. 359, Issue 6376 https://doi.org/10.1126/science.aan6398	journal	February 2018
Accurate prediction of protein structures and interactions using a three-track neural network Baek, Minkyung; DiMaio, Frank; Anishchenko, Ivan Science, Vol. 373, Issue 6557 https://doi.org/10.1126/science.abj8754	journal	July 2021
Binding of Cyt1Aa and Cry11Aa Toxins of Bacillus thuringiensis Serovar israelensis to Brush Border Membrane Vesicles of Tipula paludosa (Diptera: Nematocera) and Subsequent Pore Formation Oestergaard, Jesko; Ehlers, Ralf-Udo; Martínez-Ramírez, Amparo C. Applied and Environmental Microbiology, Vol. 73, Issue 11 https://doi.org/10.1128/AEM.01056-06	journal	June 2007
Oligomerization of Cry11Aa from Bacillus thuringiensis Has an Important Role in Toxicity against Aedes aegypti Muñoz-Garay, Carlos; Rodríguez-Almazán, Claudia; Aguilar, Jose N. Applied and Environmental Microbiology, Vol. 75, Issue 23 https://doi.org/10.1128/AEM.01303-09	journal	December 2009
Structure of the Functional Form of the Mosquito Larvicidal Cry4Aa Toxin from Bacillus thuringiensis at a 2.8-Angstrom Resolution Boonserm, Panadda; Mo, Min; Angsuthanasombat, Chanan Journal of Bacteriology, Vol. 188, Issue 9 https://doi.org/10.1128/JB.188.9.3391-3401.2006	journal	May 2006
Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein Del�cluse, A.; Rosso, M. L.; Ragni, A. Applied and Environmental Microbiology, Vol. 61, Issue 12 https://doi.org/10.1128/aem.61.12.4230-4235.1995	journal	December 1995
DeStripe: frequency-based algorithm for removing stripe noises from AFM images Chen, Shu-wen W.; Pellequer, Jean-Luc BMC Structural Biology, Vol. 11, Issue 1 https://doi.org/10.1186/1472-6807-11-7	journal	January 2011
Aromatic clusters in protein–protein and protein–drug complexes Lanzarotti, Esteban; Defelipe, Lucas A.; Marti, Marcelo A. Journal of Cheminformatics, Vol. 12, Issue 1 https://doi.org/10.1186/s13321-020-00437-4	journal	May 2020
Functional Analysis of Two Processed Fragments of Bacillus thuringiensis Cry11A Toxin Yamagiwa, Masashi; Sakagawa, Kohei; Sakai, Hiroshi Bioscience, Biotechnology, and Biochemistry, Vol. 68, Issue 3 https://doi.org/10.1271/bbb.68.523	journal	January 2004
Dominant Negative Phenotype of Bacillus thuringiensis Cry1Ab, Cry11Aa and Cry4Ba Mutants Suggest Hetero-Oligomer Formation among Different Cry Toxins Carmona, Daniela; Rodríguez-Almazán, Claudia; Muñoz-Garay, Carlos PLoS ONE, Vol. 6, Issue 5 https://doi.org/10.1371/journal.pone.0019952	journal	May 2011
A theoretical model of the tridimensional structure of Bacillus thuringiensis subsp. medellin Cry 11Bb toxin deduced by homology modelling Gutierrez, Pablo; Alzate, Oscar; Orduz, Sergio Memórias do Instituto Oswaldo Cruz, Vol. 96, Issue 3 https://doi.org/10.1590/S0074-02762001000300013	journal	April 2001
Comparing Median Lethal Concentration Values Using Confidence Interval Overlap or Ratio Tests Wheeler, Matthew W.; Park, Robert M.; Bailer, A. John Environmental Toxicology and Chemistry, Vol. 25, Issue 5 https://doi.org/10.1897/05-320R.1	journal	January 2006
Bacillus Thuringiensis Serovariety Israelensis and Bacillus Sphaericus for Mosquito Control Lacey, Lawrence A. Journal of the American Mosquito Control Association, Vol. 23, Issue sp2 https://doi.org/10.2987/8756-971X(2007)23[133:BTSIAB]2.0.CO;2	journal	July 2007
Structural Insights into Bacillus thuringiensis Cry, Cyt and Parasporin Toxins Xu, Chengchen; Wang, Bi-Cheng; Yu, Ziniu Toxins, Vol. 6, Issue 9 https://doi.org/10.3390/toxins6092732	journal	September 2014

Similar Records

Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade

Journal Article · 2020 · Nature Communications · OSTI ID:1617037

Tetreau, Guillaume; Banneville, Anne-Sophie; Andreeva, Elena A.; +35 more

Specificity of Bacillus thuringiensis endotoxins is correlated with the presence of high-affinity binding sites in the brush border membrane of target insect midguts

Journal Article · 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA) · OSTI ID:5478522

Hofmann, C; Vanderbruggen, H; Hoefte, H; +3 more

Cry1A(b)16 toxin from Bacillus thuringiensis : Theoretical refinement of three‐dimensional structure and prediction of peptides as molecular markers for detection of genetically modified organisms

Journal Article · 2017 · Proteins · OSTI ID:1400623

Plácido, Alexandra; Coelho, Andreia; Abreu Nascimento, Lucas; +9 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
bacterial toxins
nanocrystallography

Title: De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Citation Formats

References (91)

Similar Records

Related Subjects