Microfluidic contact printing: a versatile printing platform for patterning biomolecules on hydrogel substrates

Zhang, Huaibin; Hanson Shepherd, Jennifer N.; Nuzzo, Ralph G.

doi:10.1039/c000385a

Title: Microfluidic contact printing: a versatile printing platform for patterning biomolecules on hydrogel substrates

Journal Article · 06 April 2010 · Soft Matter

DOI: https://doi.org/10.1039/c000385a · OSTI ID:1875669

Zhang, Huaibin ^[1]; Hanson Shepherd, Jennifer N. ^[2]; Nuzzo, Ralph G. ^[2]

Univ. of Illinois at Urbana-Champaign, IL (United States); University of Illinois
Univ. of Illinois at Urbana-Champaign, IL (United States)

This work describes a printing platform that utilizes components of two complementary techniques—microcontact printing and microfluidic patterning—to provide a high-throughput, reusable tool for patterning biomolecularly responsive chemical features on soft materials. Hydrogel substrates imprinted with biomolecular targets (e.g., proteins or peptides) are of considerable interest because of their utility in diverse bioanalytical applications such as diagnostics and studies of cells in culture. In the system described here, a track-etched polycarbonate membrane is used to seal a PDMS microfluidic channel device to form a print head. Model ‘inks’—solutions of biotin labeled biomolecular targets—are constantly replenished via perfusion of solution through the membrane and captured on a streptavidin-incorporating polyacrylamide hydrogel-coated substrate placed in conformal contact with the print head. Furthermore, the patterns obtained can be controlled through modifications of channel design and secondary programming via selective membrane wetting. Multiple channel designs have been used to pattern three model classes of biomolecular inks (i.e., peptides, polysaccharides, and proteins). Hydrogels patterned with polylysine are used to illustrate a specific biological application for this soft-patterning method, in this case directing in vitro primary mammalian hippocampal neuronal growth. The short cycle time and reproducibility of this soft-patterning technique are highlighted.

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Research Organization:: Univ. of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: National Science Foundation; USDOE Office of Science (SC), Basic Energy Sciences (BES). Materials Sciences & Engineering Division

Grant/Contract Number:: FG02-07ER46471

OSTI ID:: 1875669

Journal Information:: Soft Matter, Journal Name: Soft Matter Journal Issue: 10 Vol. 6; ISSN 1744-683X

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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