Revealing the mechanism of lignin re-polymerization inhibitor in acidic pretreatment and its impact on enzymatic hydrolysis

He, Juan; Huang, Caoxing; Lai, Chenhuan; Wang, Zhongkai; Yuan, Liang; Ragauskas, Arthur; Yan, Youxian; Yong, Qiang

doi:10.1016/j.indcrop.2022.114631

Title: Revealing the mechanism of lignin re-polymerization inhibitor in acidic pretreatment and its impact on enzymatic hydrolysis

Journal Article · 15 February 2022 · Industrial Crops and Products

DOI: https://doi.org/10.1016/j.indcrop.2022.114631 · OSTI ID:1870228

He, Juan ^[1]; Huang, Caoxing ^[2]; Lai, Chenhuan ^[2]; Wang, Zhongkai ^[1]; Yuan, Liang ^[1];

^[3]; Yan, Youxian ^[1]; Yong, Qiang ^[2]

Anhui Agricultural University, Hefei (China)
Nanjing Forestry Univ. (China)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Phenolic additives, including 2-naphthol, 2-naphthol-7-sulfonate, and resorcinol were evaluated for their impact on the lignin re-polymerization and subsequent hydrolysis of bamboo residues. Low surface lignin concentration (65.68%) of the substrate and high Tg value (185 °C) of lignin was observed for 2-naphthol-7-sulfonate assisted pretreatment, leading to the most significant increase (14%) in enzymatic hydrolysis efficiency of pretreated bamboo residues. Meanwhile, physicochemical properties of lignin revealed that the introduced sulfonic acid groups increased the surface charge, decreased the molecular weight, promoted the cleavage of β-O-4 linkages, and prevented the re-condensation of lignin. Even though resorcinol-assisted pretreatment, by contrast, was found to be an inhibitor for enzymatic hydrolysis. NMR analysis of modified lignin showed that resorcinol led to a higher amount of non-condensed phenolic OH groups and promoted the cleavage of β-O-4 linkages, acting as an inhibitor in lignin re-polymerization during the dilute acidic pretreatment. In short, the above findings present a detailed view of suppressing lignin re-polymerization for boosting the utilization of bioresources with acidic pretreatment.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: National Natural Science Foundation of China; Shenzhen Government Plan of Science and Technology; USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1870228

Journal Information:: Industrial Crops and Products, Journal Name: Industrial Crops and Products Journal Issue: 1 Vol. 179; ISSN 0926-6690

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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