Zng1 is a GTP-dependent zinc transferase needed for activation of methionine aminopeptidase

Pasquini, Miriam; Grosjean, Nicolas; Hixson, Kim K.; Nicora, Carrie D.; Yee, Estella F.; Lipton, Mary; Blaby, Ian K.; Haley, John D.; Blaby-Haas, Crysten E.

doi:10.1016/j.celrep.2022.110834

Title: Zng1 is a GTP-dependent zinc transferase needed for activation of methionine aminopeptidase

Journal Article · 16 May 2022 · Cell Reports

DOI: https://doi.org/10.1016/j.celrep.2022.110834 · OSTI ID:1868509

^[1]; Grosjean, Nicolas ^[1]; Hixson, Kim K. ^[2]; Nicora, Carrie D. ^[2];

^[3]; Lipton, Mary ^[2];

^[1]; Haley, John D. ^[4];

^[5]

Brookhaven National Lab. (BNL), Upton, NY (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States). National Synchrotron Light Source II (NSLS-II)
Stony Brook Univ., NY (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States); Stony Brook Univ., NY (United States)

The evolution of zinc (Zn) as a protein cofactor altered the functional landscape of biology, but dependency on Zn also created an Achilles' heel, necessitating adaptive mechanisms to ensure Zn availability to proteins. A debated strategy is whether metallochaperones exist to prioritize essential Zn-dependent proteins. Here, we present evidence for a conserved family of putative metal transferases in human and fungi, which interact with Zn-dependent methionine aminopeptidase type I (MetAP1/Map1p/Fma1). Deletion of the putative metal transferase in Saccharomyces cerevisiae (ZNG1; formerly YNR029c) leads to defective Map1p function and a Zn-deficiency growth defect. In vitro, Zng1p can transfer Zn²⁺ or Co²⁺ to apo-Map1p, but unlike characterized copper chaperones, transfer is dependent on GTP hydrolysis. Proteomics reveal mis-regulation of the Zap1p transcription factor regulon due to loss of ZNG1 and Map1p activity, suggesting that Zng1p is required to avoid a compounding effect of Map1p dysfunction on survival during Zn limitation.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Laboratory Directed Research and Development (LDRD) Program

Grant/Contract Number:: SC0012704; AC02-05CH11231

OSTI ID:: 1868509

Alternate ID(s):: OSTI ID: 1882662; OSTI ID: 1925193

Report Number(s):: BNL-222969-2022-JAAM

Journal Information:: Cell Reports, Journal Name: Cell Reports Journal Issue: 7 Vol. 39; ISSN 2211-1247

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

References (77)

Spectral Probabilities and Generating Functions of Tandem Mass Spectra: A Strike against Decoy Databases Kim, Sangtae; Gupta, Nitin; Pevzner, Pavel A. Journal of Proteome Research, Vol. 7, Issue 8 https://doi.org/10.1021/pr8001244	journal	August 2008
Architecture of the human interactome defines protein communities and disease networks Huttlin, Edward L.; Bruckner, Raphael J.; Paulo, Joao A. Nature, Vol. 545, Issue 7655 https://doi.org/10.1038/nature22366	journal	May 2017
Zinc and its role in immunity and inflammation Bonaventura, Paola; Benedetti, Giulia; Albarède, Francis Autoimmunity Reviews, Vol. 14, Issue 4 https://doi.org/10.1016/j.autrev.2014.11.008	journal	April 2015
MASIC: A software program for fast quantitation and flexible visualization of chromatographic profiles from detected LC–MS(/MS) features Monroe, Matthew E.; Shaw, Jason L.; Daly, Don S. Computational Biology and Chemistry, Vol. 32, Issue 3 https://doi.org/10.1016/j.compbiolchem.2008.02.006	journal	June 2008
Catalysis and Inhibition of Mycobacterium tuberculosis Methionine Aminopeptidase Lu, Jing-Ping; Chai, Sergio C.; Ye, Qi-Zhuang Journal of Medicinal Chemistry, Vol. 53, Issue 3 https://doi.org/10.1021/jm901624n	journal	December 2009
Genome-wide characterization of the Zap1p zinc-responsive regulon in yeast Lyons, T. J.; Gasch, A. P.; Gaither, L. A. Proceedings of the National Academy of Sciences, Vol. 97, Issue 14 https://doi.org/10.1073/pnas.97.14.7957	journal	July 2000
Automated comparative protein structure modeling with SWISS-MODEL and Swiss-PdbViewer: A historical perspective Guex, Nicolas; Peitsch, Manuel C.; Schwede, Torsten ELECTROPHORESIS, Vol. 30, Issue S1 https://doi.org/10.1002/elps.200900140	journal	June 2009
Yeast Methionine Aminopeptidase I Walker, Kenneth W.; Bradshaw, Ralph A. Journal of Biological Chemistry, Vol. 274, Issue 19 https://doi.org/10.1074/jbc.274.19.13403	journal	May 1999
The Specificity in Vivo of Two Distinct Methionine Aminopeptidases in Saccharomyces cerevisiae Chen, Shaoping; Vetro, Joseph A.; Chang, Yie-Hwa Archives of Biochemistry and Biophysics, Vol. 398, Issue 1 https://doi.org/10.1006/abbi.2001.2675	journal	February 2002
The Phyre2 web portal for protein modeling, prediction and analysis Kelley, Lawrence A.; Mezulis, Stefans; Yates, Christopher M. Nature Protocols, Vol. 10, Issue 6 https://doi.org/10.1038/nprot.2015.053	journal	May 2015
BioGRID: a general repository for interaction datasets Stark, C. Nucleic Acids Research, Vol. 34, Issue 90001 https://doi.org/10.1093/nar/gkj109	journal	January 2006
NIH Image to ImageJ: 25 years of image analysis Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W. Nature Methods, Vol. 9, Issue 7 https://doi.org/10.1038/nmeth.2089	journal	June 2012
The yeast ZRT1 gene encodes the zinc transporter protein of a high-affinity uptake system induced by zinc limitation. Zhao, H.; Eide, D. Proceedings of the National Academy of Sciences, Vol. 93, Issue 6 https://doi.org/10.1073/pnas.93.6.2454	journal	March 1996
Jalview Version 2--a multiple sequence alignment editor and analysis workbench Waterhouse, A. M.; Procter, J. B.; Martin, D. M. A. Bioinformatics, Vol. 25, Issue 9 https://doi.org/10.1093/bioinformatics/btp033	journal	January 2009
COG0523 proteins: a functionally diverse family of transition metal-regulated G3E P-loop GTP hydrolases from bacteria to man Edmonds, Katherine A.; Jordan, Matthew R.; Giedroc, David P. Metallomics, Vol. 13, Issue 8 https://doi.org/10.1093/mtomcs/mfab046	journal	July 2021
Counting the Zinc-Proteins Encoded in the Human Genome Andreini, Claudia; Banci, Lucia; Bertini, Ivano Journal of Proteome Research, Vol. 5, Issue 1 https://doi.org/10.1021/pr050361j	journal	January 2006
A global genetic interaction network maps a wiring diagram of cellular function Costanzo, M.; VanderSluis, B.; Koch, E. N. Science, Vol. 353, Issue 6306 https://doi.org/10.1126/science.aaf1420	journal	September 2016
Evidence that two zinc fingers in the methionine aminopeptidase from Saccharomyces cerevisiae are important for normal growth Zuo, Songlan; Guo, Qi; Ling, Cliff Molecular and General Genetics MGG, Vol. 246, Issue 2 https://doi.org/10.1007/BF00294688	journal	March 1995
SWISS-MODEL: homology modelling of protein structures and complexes Waterhouse, Andrew; Bertoni, Martino; Bienert, Stefan Nucleic Acids Research, Vol. 46, Issue W1 https://doi.org/10.1093/nar/gky427	journal	May 2018
Metal Preferences and Metallation Foster, Andrew W.; Osman, Deenah; Robinson, Nigel J. Journal of Biological Chemistry, Vol. 289, Issue 41 https://doi.org/10.1074/jbc.R114.588145	journal	August 2014
YeiR: a metal-binding GTPase from Escherichia coli involved in metal homeostasis Blaby-Haas, Crysten E.; Flood, Jessica A.; Crécy-Lagard, Valérie de Metallomics, Vol. 4, Issue 5 https://doi.org/10.1039/c2mt20012k	journal	January 2012
edgeR: a Bioconductor package for differential expression analysis of digital gene expression data Robinson, M. D.; McCarthy, D. J.; Smyth, G. K. Bioinformatics, Vol. 26, Issue 1 https://doi.org/10.1093/bioinformatics/btp616	journal	November 2009
Arabidopsis C‐terminal binding protein ANGUSTIFOLIA modulates transcriptional co‐regulation of MYB46 and WRKY33 Xie, Meng; Zhang, Jin; Yao, Tao New Phytologist, Vol. 228, Issue 5 https://doi.org/10.1111/nph.16826	journal	August 2020
Functional profiling of the Saccharomyces cerevisiae genome Giaever, Guri; Chu, Angela M.; Ni, Li Nature, Vol. 418, Issue 6896 https://doi.org/10.1038/nature00935	journal	July 2002
Amino-terminal protein processing in Saccharomyces cerevisiae is an essential function that requires two distinct methionine aminopeptidases. Li, X.; Chang, Y. H. Proceedings of the National Academy of Sciences, Vol. 92, Issue 26 https://doi.org/10.1073/pnas.92.26.12357	journal	December 1995
Evolution of a plant-specific copper chaperone family for chloroplast copper homeostasis Blaby-Haas, Crysten E.; Padilla-Benavides, Teresita; Stübe, Roland Proceedings of the National Academy of Sciences, Vol. 111, Issue 50 https://doi.org/10.1073/pnas.1421545111	journal	December 2014
Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds Mumberg, Dominik; Müller, Rolf; Funk, Martin Gene, Vol. 156, Issue 1 https://doi.org/10.1016/0378-1119(95)00037-7	journal	April 1995
A subset of the diverse COG0523 family of putative metal chaperones is linked to zinc homeostasis in all kingdoms of life Haas, Crysten E.; Rodionov, Dmitry A.; Kropat, Janette BMC Genomics, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2164-10-470	journal	January 2009
Yeast methionine aminopeptidase I can utilize either Zn ²⁺ or Co ²⁺ as a cofactor: A case of mistaken identity? Walker, Kenneth W.; Bradshaw, Ralph A. Protein Science, Vol. 7, Issue 12 https://doi.org/10.1002/pro.5560071224	journal	December 1998
A new micromethod for the colorimetric determination of inorganic phosphate Itaya, Koichi; Ui, Michio Clinica Chimica Acta, Vol. 14, Issue 3 https://doi.org/10.1016/0009-8981(66)90114-8	journal	September 1966
The DISOPRED server for the prediction of protein disorder Ward, J. J.; McGuffin, L. J.; Bryson, K. Bioinformatics, Vol. 20, Issue 13 https://doi.org/10.1093/bioinformatics/bth195	journal	March 2004
A pathology atlas of the human cancer transcriptome Uhlen, Mathias; Zhang, Cheng; Lee, Sunjae Science, Vol. 357, Issue 6352 https://doi.org/10.1126/science.aan2507	journal	August 2017
Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells Wang, Yuexi; Yang, Feng; Gritsenko, Marina A. PROTEOMICS, Vol. 11, Issue 10 https://doi.org/10.1002/pmic.201000722	journal	April 2011
Estimating the Global Prevalence of Zinc Deficiency: Results Based on Zinc Availability in National Food Supplies and the Prevalence of Stunting Wessells, K. Ryan; Brown, Kenneth H. PLoS ONE, Vol. 7, Issue 11 https://doi.org/10.1371/journal.pone.0050568	journal	November 2012
A Triple Knockout (TKO) Proteomics Standard for Diagnosing Ion Interference in Isobaric Labeling Experiments Paulo, Joao A.; O’Connell, Jeremy D.; Gygi, Steven P. Journal of The American Society for Mass Spectrometry, Vol. 27, Issue 10 https://doi.org/10.1007/s13361-016-1434-9	journal	July 2016
clusterProfiler: an R Package for Comparing Biological Themes Among Gene Clusters Yu, Guangchuang; Wang, Li-Gen; Han, Yanyan OMICS: A Journal of Integrative Biology, Vol. 16, Issue 5 https://doi.org/10.1089/omi.2011.0118	journal	May 2012
An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database Eng, Jimmy K.; McCormack, Ashley L.; Yates, John R. Journal of the American Society for Mass Spectrometry, Vol. 5, Issue 11 https://doi.org/10.1016/1044-0305(94)80016-2	journal	November 1994
UniProt: a worldwide hub of protein knowledge Consortium, The UniProt Nucleic Acids Research https://doi.org/10.1093/nar/gky1049		November 2018
Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction Networks Shannon, P. Genome Research, Vol. 13, Issue 11 https://doi.org/10.1101/gr.1239303	journal	November 2003
Computed structures of core eukaryotic protein complexes Humphreys, Ian R.; Pei, Jimin; Baek, Minkyung Science, Vol. 374, Issue 6573 https://doi.org/10.1126/science.abm4805	journal	December 2021
Target-Decoy Search Strategy for Mass Spectrometry-Based Proteomics Elias, Joshua E.; Gygi, Steven P. Methods in Molecular Biology https://doi.org/10.1007/978-1-60761-444-9_5	book	December 2009
Classification and evolution of P-loop GTPases and related ATPases Leipe, Detlef D.; Wolf, Yuri I.; Koonin, Eugene V. Journal of Molecular Biology, Vol. 317, Issue 1 https://doi.org/10.1006/jmbi.2001.5378	journal	March 2002
Proteomics-based Target Identification Towbin, Harry; Bair, Kenneth W.; DeCaprio, James A. Journal of Biological Chemistry, Vol. 278, Issue 52 https://doi.org/10.1074/jbc.M309039200	journal	December 2003
Databases of homologous gene families for comparative genomics Penel, Simon; Arigon, Anne-Muriel; Dufayard, Jean-François BMC Bioinformatics, Vol. 10, Issue Suppl 6 https://doi.org/10.1186/1471-2105-10-S6-S3	journal	January 2009
Purification and Characterization of an Aminopeptidase from Lactococcus lactis subsp. cremoris Wg2 Tan, P. S. T.; Konings, W. N. Applied and Environmental Microbiology, Vol. 56, Issue 2 https://doi.org/10.1128/aem.56.2.526-532.1990	journal	February 1990
Identification of the Human Zinc Transcriptional Regulatory Element (ZTRE) Coneyworth, Lisa J.; Jackson, Kelly A.; Tyson, John Journal of Biological Chemistry, Vol. 287, Issue 43 https://doi.org/10.1074/jbc.M112.397000	journal	October 2012
Interactive Tree Of Life (iTOL) v4: recent updates and new developments Letunic, Ivica; Bork, Peer Nucleic Acids Research, Vol. 47, Issue W1 https://doi.org/10.1093/nar/gkz239	journal	April 2019
Solvent extraction-spectrophotometric determination of phosphate with molybdate and malachite green in river water and sea-water Motomizu, S. Talanta, Vol. 31, Issue 4 https://doi.org/10.1016/0039-9140(84)80269-6	journal	April 1984
UCSF ChimeraX: Meeting modern challenges in visualization and analysis: UCSF ChimeraX Visualization System Goddard, Thomas D.; Huang, Conrad C.; Meng, Elaine C. Protein Science, Vol. 27, Issue 1 https://doi.org/10.1002/pro.3235	journal	September 2017
COBALT: constraint-based alignment tool for multiple protein sequences Papadopoulos, J. S.; Agarwala, R. Bioinformatics, Vol. 23, Issue 9 https://doi.org/10.1093/bioinformatics/btm076	journal	March 2007
Zinc Deficiency Impacts CO ₂ Assimilation and Disrupts Copper Homeostasis in Chlamydomonas reinhardtii Malasarn, Davin; Kropat, Janette; Hsieh, Scott I. Journal of Biological Chemistry, Vol. 288, Issue 15 https://doi.org/10.1074/jbc.M113.455105	journal	February 2013
Progression from remodeling to hibernation of ribosomes in zinc-starved mycobacteria Li, Yunlong; Corro, Jamie H.; Palmer, Christopher D. Proceedings of the National Academy of Sciences, Vol. 117, Issue 32 https://doi.org/10.1073/pnas.2013409117	journal	July 2020
Decoding the Function of Expansion Segments in Ribosomes Fujii, Kotaro; Susanto, Teodorus Theo; Saurabh, Saumya Molecular Cell, Vol. 72, Issue 6 https://doi.org/10.1016/j.molcel.2018.11.023	journal	December 2018
Deciphering key features in protein structures with the new ENDscript server Robert, Xavier; Gouet, Patrice Nucleic Acids Research, Vol. 42, Issue W1 https://doi.org/10.1093/nar/gku316	journal	April 2014
FastTree 2 – Approximately Maximum-Likelihood Trees for Large Alignments Price, Morgan N.; Dehal, Paramvir S.; Arkin, Adam P. PLoS ONE, Vol. 5, Issue 3 https://doi.org/10.1371/journal.pone.0009490	journal	March 2010
Mechanism of Cu+-transporting ATPases: Soluble Cu+ chaperones directly transfer Cu+ to transmembrane transport sites Gonzalez-Guerrero, M.; Arguello, J. M. Proceedings of the National Academy of Sciences, Vol. 105, Issue 16 https://doi.org/10.1073/pnas.0711446105	journal	April 2008
Zap1p, a metalloregulatory protein involved in zinc-responsive transcriptional regulation in Saccharomyces cerevisiae Zhao, H.; Eide, D. J. Molecular and Cellular Biology, Vol. 17, Issue 9 https://doi.org/10.1128/MCB.17.9.5044	journal	September 1997
Mechanistic Insights into the Metal-Dependent Activation of Zn ^II -Dependent Metallochaperones Jordan, Matthew R.; Wang, Jiefei; Weiss, Andy Inorganic Chemistry, Vol. 58, Issue 20 https://doi.org/10.1021/acs.inorgchem.9b01173	journal	June 2019
The Response of Acinetobacter baumannii to Zinc Starvation Nairn, Brittany L.; Lonergan, Zachery R.; Wang, Jiefei Cell Host & Microbe, Vol. 19, Issue 6 https://doi.org/10.1016/j.chom.2016.05.007	journal	June 2016
Yeast Glutamine-fructose-6-phosphate Aminotransferase (Gfa1) Requires Methionine Aminopeptidase Activity for Proper Function Dummitt, Benjamin; Micka, William S.; Chang, Yie-Hwa Journal of Biological Chemistry, Vol. 280, Issue 14 https://doi.org/10.1074/jbc.M501059200	journal	April 2005
Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and bifunctional cobinamide kinase-cobinamide phosphate guanylyltransferase Crouzet, J.; Levy-Schil, S.; Cameron, B. Journal of Bacteriology, Vol. 173, Issue 19 https://doi.org/10.1128/jb.173.19.6074-6087.1991	journal	October 1991
TimeTree: A Resource for Timelines, Timetrees, and Divergence Times Kumar, Sudhir; Stecher, Glen; Suleski, Michael Molecular Biology and Evolution, Vol. 34, Issue 7 https://doi.org/10.1093/molbev/msx116	journal	April 2017
Deletion in the Cobalamin Synthetase W Domain–Containing Protein 1 Gene Is associated with Congenital Anomalies of the Kidney and Urinary Tract Kanda, Shoichiro; Ohmuraya, Masaki; Akagawa, Hiroyuki Journal of the American Society of Nephrology, Vol. 31, Issue 1 https://doi.org/10.1681/ASN.2019040398	journal	December 2019
Zinc depletion induces ribosome hibernation in mycobacteria Li, Yunlong; Sharma, Manjuli R.; Koripella, Ravi K. Proceedings of the National Academy of Sciences, Vol. 115, Issue 32 https://doi.org/10.1073/pnas.1804555115	journal	July 2018
UniRef clusters: a comprehensive and scalable alternative for improving sequence similarity searches Suzek, B. E.; Wang, Y.; Huang, H. Bioinformatics, Vol. 31, Issue 6 https://doi.org/10.1093/bioinformatics/btu739	journal	November 2014
The cellular economy of the Saccharomyces cerevisiae zinc proteome Wang, Yirong; Weisenhorn, Erin; MacDiarmid, Colin W. Metallomics, Vol. 10, Issue 12 https://doi.org/10.1039/C8MT00269J	journal	January 2018
The BioPlex Network: A Systematic Exploration of the Human Interactome Huttlin, Edward L.; Ting, Lily; Bruckner, Raphael J. Cell, Vol. 162, Issue 2 https://doi.org/10.1016/j.cell.2015.06.043	journal	July 2015
Microbial nickel: cellular uptake and delivery to enzyme centers Zeer-Wanklyn, Conor J.; Zamble, Deborah B. Current Opinion in Chemical Biology, Vol. 37 https://doi.org/10.1016/j.cbpa.2017.01.014	journal	April 2017
Hidden shift of the ionome of plants exposed to elevated CO2 depletes minerals at the base of human nutrition Loladze, Irakli eLife, Vol. 3 https://doi.org/10.7554/eLife.02245	journal	May 2014
High-Quality Binary Protein Interaction Map of the Yeast Interactome Network Yu, H.; Braun, P.; Yildirim, M. A. Science, Vol. 322, Issue 5898 https://doi.org/10.1126/science.1158684	journal	October 2008
Discovery of Human Zinc Deficiency: Its Impact on Human Health and Disease Prasad, Ananda S. Advances in Nutrition, Vol. 4, Issue 2 https://doi.org/10.3945/an.112.003210	journal	March 2013
Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks Gerlt, John A.; Bouvier, Jason T.; Davidson, Daniel B. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1854, Issue 8 https://doi.org/10.1016/j.bbapap.2015.04.015	journal	August 2015
A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human Vo, Tommy V.; Das, Jishnu; Meyer, Michael J. Cell, Vol. 164, Issue 1-2 https://doi.org/10.1016/j.cell.2015.11.037	journal	January 2016
Structural insights into how GTP-dependent conformational changes in a metallochaperone UreG facilitate urease maturation Yuen, Man Hon; Fong, Yu Hang; Nim, Yap Shing Proceedings of the National Academy of Sciences, Vol. 114, Issue 51 https://doi.org/10.1073/pnas.1712658114	journal	December 2017
Bacillus subtilis FolE is sustained by the ZagA zinc metallochaperone and the alarmone ZTP under conditions of zinc deficiency Chandrangsu, Pete; Huang, Xiaojuan; Gaballa, Ahmed Molecular Microbiology, Vol. 112, Issue 3 https://doi.org/10.1111/mmi.14314	journal	June 2019
Physiologically Relevant Metal Cofactor for Methionine Aminopeptidase-2 Is Manganese Wang, Jieyi; Sheppard, George S.; Lou, Pingping Biochemistry, Vol. 42, Issue 17 https://doi.org/10.1021/bi020670c	journal	May 2003
GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins Soriano, A.; Hausinger, R. P. Proceedings of the National Academy of Sciences, Vol. 96, Issue 20 https://doi.org/10.1073/pnas.96.20.11140	journal	September 1999

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59 BASIC BIOLOGICAL SCIENCES
MetAP
insertase
NME
GTPase
zinc
homeostasis
nutrient limitation
COG0523
CobW
CBWD

Title: Zng1 is a GTP-dependent zinc transferase needed for activation of methionine aminopeptidase

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