In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera
Abstract
Near-infrared (NIR) fluorescence lifetime imaging (FLI) provides a unique contrast mechanism to monitor biological parameters and molecular events in vivo. Single-photon avalanche diode (SPAD) cameras have been recently demonstrated in FLI microscopy (FLIM) applications, but their suitability for in vivo macroscopic FLI (MFLI) in deep tissues remains to be demonstrated. Herein, we report in vivo NIR MFLI measurement with SwissSPAD2, a large time-gated SPAD camera. We first benchmark its performance in well-controlled in vitro experiments, ranging from monitoring environmental effects on fluorescence lifetime, to quantifying Förster resonant energy transfer (FRET) between dyes. Next, we use it for in vivo studies of target-drug engagement in live and intact tumor xenografts using FRET. Information obtained with SwissSPAD2 was successfully compared to that obtained with a gated intensified charge-coupled device (ICCD) camera, using two different approaches. Our results demonstrate that SPAD cameras offer a powerful technology for in vivo preclinical applications in the NIR window.
- Publication Date:
- Research Org.:
- Univ. of California, Los Angeles, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); National Institutes of Health (NIH); Jonsson Comprehensive Cancer Center Seed Grant Program; Swiss National Science Foundation (SNSF)
- OSTI Identifier:
- 1867073
- Alternate Identifier(s):
- OSTI ID: 1980987; OSTI ID: 2228967
- Grant/Contract Number:
- SC0020338; R01CA207725; R01CA237267; R01CA250636
- Resource Type:
- Published Article
- Journal Name:
- Optica
- Additional Journal Information:
- Journal Name: Optica Journal Volume: 9 Journal Issue: 5; Journal ID: ISSN 2334-2536
- Publisher:
- Optical Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION
Citation Formats
Smith, Jason T., Rudkouskaya, Alena, Gao, Shan, Gupta, Juhi M., Ulku, Arin, Bruschini, Claudio, Charbon, Edoardo, Weiss, Shimon, Barroso, Margarida, Intes, Xavier, and Michalet, Xavier. In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera. United States: N. p., 2022.
Web. doi:10.1364/OPTICA.454790.
Smith, Jason T., Rudkouskaya, Alena, Gao, Shan, Gupta, Juhi M., Ulku, Arin, Bruschini, Claudio, Charbon, Edoardo, Weiss, Shimon, Barroso, Margarida, Intes, Xavier, & Michalet, Xavier. In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera. United States. https://doi.org/10.1364/OPTICA.454790
Smith, Jason T., Rudkouskaya, Alena, Gao, Shan, Gupta, Juhi M., Ulku, Arin, Bruschini, Claudio, Charbon, Edoardo, Weiss, Shimon, Barroso, Margarida, Intes, Xavier, and Michalet, Xavier. Mon .
"In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera". United States. https://doi.org/10.1364/OPTICA.454790.
@article{osti_1867073,
title = {In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera},
author = {Smith, Jason T. and Rudkouskaya, Alena and Gao, Shan and Gupta, Juhi M. and Ulku, Arin and Bruschini, Claudio and Charbon, Edoardo and Weiss, Shimon and Barroso, Margarida and Intes, Xavier and Michalet, Xavier},
abstractNote = {Near-infrared (NIR) fluorescence lifetime imaging (FLI) provides a unique contrast mechanism to monitor biological parameters and molecular events in vivo. Single-photon avalanche diode (SPAD) cameras have been recently demonstrated in FLI microscopy (FLIM) applications, but their suitability for in vivo macroscopic FLI (MFLI) in deep tissues remains to be demonstrated. Herein, we report in vivo NIR MFLI measurement with SwissSPAD2, a large time-gated SPAD camera. We first benchmark its performance in well-controlled in vitro experiments, ranging from monitoring environmental effects on fluorescence lifetime, to quantifying Förster resonant energy transfer (FRET) between dyes. Next, we use it for in vivo studies of target-drug engagement in live and intact tumor xenografts using FRET. Information obtained with SwissSPAD2 was successfully compared to that obtained with a gated intensified charge-coupled device (ICCD) camera, using two different approaches. Our results demonstrate that SPAD cameras offer a powerful technology for in vivo preclinical applications in the NIR window.},
doi = {10.1364/OPTICA.454790},
journal = {Optica},
number = 5,
volume = 9,
place = {United States},
year = {Mon May 09 00:00:00 EDT 2022},
month = {Mon May 09 00:00:00 EDT 2022}
}
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