Comparative Phenotyping of Two Commonly Used Chlamydomonas reinhardtii Background Strains: CC-1690 (21gr) and CC-5325 (The CLiP Mutant Library Background)

Zhang, Ningning; Pazouki, Leila; Nguyen, Huong; Jacobshagen, Sigrid; Bigge, Brae M.; Xia, Ming; Mattoon, Erin M.; Klebanovych, Anastasiya; Sorkin, Maria; Nusinow, Dmitri A.; Avasthi, Prachee; Czymmek, Kirk J.; Zhang, Ru

doi:10.3390/plants11050585

Title: Comparative Phenotyping of Two Commonly Used Chlamydomonas reinhardtii Background Strains: CC-1690 (21gr) and CC-5325 (The CLiP Mutant Library Background)

Journal Article · 21 February 2022 · Plants

DOI: https://doi.org/10.3390/plants11050585 · OSTI ID:1864761

Zhang, Ningning ^[1]; Pazouki, Leila ^[2]; Nguyen, Huong ^[2]; Jacobshagen, Sigrid ^[3];

^[4]; Xia, Ming ^[2]; Mattoon, Erin M. ^[5];

^[2];

^[5];

^[2];

^[4];

^[2];

^[2]

Donald Danforth Plant Science Center, St. Louis, MO (United States); Donald Danforth Plant Science Center
Donald Danforth Plant Science Center, St. Louis, MO (United States)
Western Kentucky University, Bowling Green, KY (United States)
Dartmouth College, Hanover, NH (United States). Geisel School of Medicine
Donald Danforth Plant Science Center, St. Louis, MO (United States); Washington Univ., St. Louis, MO (United States)

The unicellular green alga Chlamydomonas reinhardtii is an excellent model organism to investigate many essential cellular processes in photosynthetic eukaryotes. Two commonly used background strains of Chlamydomonas are CC-1690 and CC-5325. CC-1690, also called 21gr, has been used for the Chlamydomonas genome project and several transcriptome analyses. CC-5325 is the background strain for the Chlamydomonas Library Project (CLiP). Photosynthetic performance in CC-5325 has not been evaluated in comparison with CC-1690. Additionally, CC-5325 is often considered to be cell-wall deficient, although detailed analysis is missing. The circadian rhythms in CC-5325 are also unclear. To fill these knowledge gaps and facilitate the use of the CLiP mutant library for various screens, we performed phenotypic comparisons between CC-1690 and CC-5325. Our results showed that CC-5325 grew faster heterotrophically in dark and equally well in mixotrophic liquid medium as compared to CC-1690. CC-5325 had lower photosynthetic efficiency and was more heat-sensitive than CC-1690. Furthermore, CC-5325 had an intact cell wall which had comparable integrity to that in CC-1690 but appeared to have reduced thickness. Additionally, CC-5325 could perform phototaxis, but could not maintain a sustained circadian rhythm of phototaxis as CC1690 did. Finally, in comparison to CC-1690, CC-5325 had longer cilia in the medium with acetate but slower swimming speed in the medium without nitrogen and acetate. Our results will be useful for researchers in the Chlamydomonas community to choose suitable background strains for mutant analysis and employ the CLiP mutant library for genome-wide mutant screens under appropriate conditions, especially in the areas of photosynthesis, thermotolerance, cell wall, and circadian rhythms.

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Research Organization:: Donald Danforth Plant Science Center, St. Louis, MO (United States)

Sponsoring Organization:: National Institute of General Medical Sciences (NIGMS); National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0019464; SC0020400

OSTI ID:: 1864761

Journal Information:: Plants, Journal Name: Plants Journal Issue: 5 Vol. 11; ISSN 2223-7747

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis Jiang, Xingshan; Stern, David Journal of Visualized Experiments, Issue 30 https://doi.org/10.3791/1274-v	journal	August 2009
Co-expression networks in Chlamydomonas reveal significant rhythmicity in batch cultures and empower gene function discovery Salomé, Patrice; Merchant, Sabeeha Dryad https://doi.org/10.5068/d1wd55	dataset	January 2021
The Mars1 kinase confers photoprotection through signaling in the chloroplast unfolded protein response Perlaza, Karina; Toutkoushian, Hannah; Boone, Morgane eLife, Vol. 8 https://doi.org/10.7554/eLife.49577	journal	October 2019

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59 BASIC BIOLOGICAL SCIENCES
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