Catalytic DNA Polymerization Can Be Expedited by Active Product Release**

Moerman, Pepijn G.; Gavrilov, Momcilo; Ha, Taekjip; Schulman, Rebecca

doi:10.1002/ange.202114581

Title: Catalytic DNA Polymerization Can Be Expedited by Active Product Release**

Journal Article · 19 April 2022 · Angewandte Chemie

DOI: https://doi.org/10.1002/ange.202114581 · OSTI ID:1864080

^[1]; Gavrilov, Momcilo ^[2]; Ha, Taekjip ^[3];

^[4]

Chemical and Biomolecular Engineering Johns Hopkins University Baltimore MD 21218 USA
Biophysics &, Biophysical Chemistry Johns Hopkins University Baltimore MD 21205 USA
Biophysics &, Biophysical Chemistry Johns Hopkins University Baltimore MD 21205 USA, Biomedical Engineering Johns Hopkins University Baltimore MD 21218 USA, Howard Hughes Medical Institute Baltimore MD 21205 USA
Chemical and Biomolecular Engineering Johns Hopkins University Baltimore MD 21218 USA, Chemistry Johns Hopkins University Baltimore MD 21218 USA, Computer Science Johns Hopkins University Baltimore MD 21218 USA

Abstract The sequence‐specific hybridization of DNA facilitates its use as a building block for designer nanoscale structures and reaction networks that perform computations. However, the strong binding energy of Watson–Crick base pairing that underlies this specificity also causes the DNA dehybridization rate to depend sensitively on sequence length and temperature. This strong dependency imposes stringent constraints on the design of multi‐step DNA reactions. Here we show how an ATP‐dependent helicase, Rep‐X, can drive specific dehybridization reactions at rates independent of sequence length, removing the constraints of equilibrium on DNA hybridization and dehybridization. To illustrate how this new capacity can speed up designed DNA reaction networks, we show that Rep‐X extends the range of conditions where the primer exchange reaction, which catalytically adds a domain provided by a hairpin template to a DNA substrate, proceeds rapidly.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0010426

OSTI ID:: 1864080

Journal Information:: Angewandte Chemie, Journal Name: Angewandte Chemie Journal Issue: 24 Vol. 134; ISSN 0044-8249

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Germany

Language:: English

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