The structure of Synechococcus elongatus enolase reveals key aspects of phosphoenolpyruvate binding
- National University of Santiago del Estero (Argentina)
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
A structure–function characterization of Synechococcus elongatus enolase (SeEN) is presented, representing the first structural report on a cyanobacterial enolase. X-ray crystal structures of SeEN in its apoenzyme form and in complex with phosphoenolpyruvate are reported at 2.05 and 2.30 Å resolution, respectively. SeEN displays the typical fold of enolases, with a conformationally flexible loop that closes the active site upon substrate binding, assisted by two metal ions that stabilize the negatively charged groups. The enzyme exhibits a catalytic efficiency of 1.2 x 105 M-1 s-1 for the dehydration of 2-phospho-D-glycerate, which is comparable to the kinetic parameters of related enzymes. These results expand the understanding of the biophysical features of these enzymes, broadening the toolbox for metabolic engineering applications.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); Natural Sciences and Engineering Research Council of Canada (NSERC); National Research Council; Canadian Institutes of Health Research (CIHR); University of Saskatchewan; USDOE
- Grant/Contract Number:
- 89233218CNA000001; AC02-76SF00515; P30GM133894
- OSTI ID:
- 1862089
- Alternate ID(s):
- OSTI ID: 1869624
- Report Number(s):
- LA-UR-22-20772
- Journal Information:
- Acta Crystallographica. Section F, Structural Biology Communications, Vol. 78, Issue 4
- Publisher:
- International Union of CrystallographyCopyright Statement
- Country of Publication:
- United States
- Language:
- English
Similar Records
Fluoride Inhibition of Enolase: Crystal Structure and Thermodynamics
Metabolic engineering of Synechococcus elongatus 7942 for enhanced sucrose biosynthesis