Title: Phosphate Availability Modulates Root Exudate Composition and Rhizosphere Microbial Community in a Teosinte and a Modern Maize Cultivar

Abstract

Domestication and breeding have affected interactions between plants and their microbiomes in ways that are only beginning to be understood but may have important implications for recruitment of rhizosphere microorganisms, particularly under stress conditions. We investigated the responses of a modern maize (Zea mays subsp. mays) cultivar and its wild relative, teosinte (Z. mays subsp. parviglumis), to different phosphate availabilities. We appraised responses of the plant-microbial holobiont to phosphate stresses by profiling root exudate metabolomes, and microbial communities in the root endosphere and rhizosphere. We also performed plate assays to quantify phosphate-solubilizing microorganisms from the rhizosphere. Although root exudate metabolite profiles were distinct between the teosinte and modern maize under high phosphate, both plants shifted exudate compositions in response to phosphate stress toward a common metabolite profile. Root and rhizosphere microbial communities also responded significantly to both plant type and the phosphate availability. A subset of bacterial and fungal taxa were differentially abundant under the different phosphate conditions, with each of the three conditions favoring different taxa. Both teosinte and maize rhizospheres harbored phosphate-solubilizing microorganisms under all growth conditions. These results suggest that the root exudation response to phosphate stress was conserved through the domestication of maize from teosinte, shiftingmore » exudation levels of specific metabolites. Although microbial communities also shifted, plate-based assays did not detect selective recruitment of phosphate solubilizers in response to phosphate availability.« less

Authors:

Brisson, Vanessa L.  ^[1];

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Richardy, Jesper ^[2]; Kosina, Suzanne M. ^[3]; Northen, Trent R. ^[4]; Vogel, John P. ^[5]; Gaudin, Amélie M. ^[2]

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+ Show Author Affiliations

1. Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
2. University of California, Davis, CA (United States)
3. Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
4. Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)
5. USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); University of California, Berkeley, CA (United States)

Publication Date:

Mon Jan 24 00:00:00 EST 2022

Research Org.:

Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC); USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)

Sponsoring Org.:

USDOE Laboratory Directed Research and Development (LDRD) Program; USDOE Office of Science (SC); USDA; National Institute of Food and Agriculture (NIFA); Foundation for Food and Agriculture Research

OSTI Identifier:

1860650

Alternate Identifier(s):

OSTI ID: 1923759

Report Number(s):

LLNL-JRNL-817896
Journal ID: ISSN 2471-2906; 1028119

Grant/Contract Number:  

AC52-07NA27344; AC02-05CH11231; CA-D-PLS-2332-H

Resource Type:

Accepted Manuscript

Journal Name:

Phytobiomes Journal

Additional Journal Information:

Journal Volume: 6; Journal Issue: 1; Journal ID: ISSN 2471-2906

Publisher:

American Phytopathological Society (APS)

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; maize; metabolomics; microbiome; phosphate-solubilizing microorganisms; phosphate stress; rhizosphere and phyllosphere; root exudates; teosinte; Zea mays