Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi

Keyes, Sam; Veelen, Arjen; McKay Fletcher, Dan; Scotson, Callum; Koebernick, Nico; Petroselli, Chiara; Williams, Katherine; Ruiz, Siul; Cooper, Laura; Mayon, Robbie; Duncan, Simon; Dumont, Marc; Jakobsen, Iver; Oldroyd, Giles; Tkacz, Andrzej; Poole, Philip; Mosselmans, Fred; Borca, Camelia; Huthwelker, Thomas; Jones, David L.; Roose, Tiina

doi:10.1111/nph.17980

Title: Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi

Journal Article · 14 February 2022 · New Phytologist

DOI: https://doi.org/10.1111/nph.17980 · OSTI ID:1856234

Keyes, Sam ^[1]; Veelen, Arjen ^[2];

^[1]; Scotson, Callum ^[1]; Koebernick, Nico ^[1]; Petroselli, Chiara ^[1]; Williams, Katherine ^[1]; Ruiz, Siul ^[1]; Cooper, Laura ^[1]; Mayon, Robbie ^[1]; Duncan, Simon ^[1]; Dumont, Marc ^[1]; Jakobsen, Iver ^[3]; Oldroyd, Giles ^[4]; Tkacz, Andrzej ^[5]; Poole, Philip ^[5]; Mosselmans, Fred ^[6]; Borca, Camelia ^[7]; Huthwelker, Thomas ^[7]; Jones, David L. ^[8] more »

Univ. of Southampton (United Kingdom)
Univ. of Southampton (United Kingdom); Los Alamos National Lab. (LANL), Los Alamos, NM (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)
Univ. of Copenhagen (Denmark)
Univ. of Cambridge (United Kingdom). Crop Science Centre
Univ. of Oxford (United Kingdom)
Science and Technology Facilities Council (STFC), Oxford (United Kingdom). Diamond Light Source, Ltd.
Paul Scherrer Inst. (PSI), Villigen (Switzerland). Swiss Light Source
Bangor Univ. (United Kingdom); Murdoch Univ., WA (Australia). Food Futures Inst.

Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore-space, and models of AMF-enhanced P-uptake are poorly validated. We used synchrotron X-ray computed tomography to visualize mycorrhizas in soil and synchrotron X-ray fluorescence/X-ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in combination with modelling. We found that AMF inoculation had a suppressive effect on colonisation by other soil fungi and identified differences in structure and growth rate between hyphae of AMF and nonmycorrhizal fungi. Our results showed that AMF co-locate with areas of high P and low Al, and preferentially associate with organic-type P species over Al-rich inorganic P. We discovered that AMF avoid Al-rich areas as a source of P. Sulphur-rich regions were found to be correlated with higher hyphal density and an increased organic-associated P-pool, whilst oxidized S-species were found close to AMF hyphae. Increased S oxidation close to AMF suggested the observed changes were microbiome-related. Our experimentally-validated model led to an estimate of P-uptake by AMF hyphae that is an order of magnitude lower than rates previously estimated – a result with significant implications for the modelling of plant–soil–AMF interactions.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: EPSRC; European Research Council (ERC); NERC; USDOE National Nuclear Security Administration (NNSA); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: 89233218CNA000001; AC02-76SF00515

OSTI ID:: 1856234

Report Number(s):: LA-UR-21-22547; 646809‐DIMR; BB/L02620/1; BB/L502625/1; BB/P004180/1; BB/N013387/1; BB/R017859/1; EP/M020355/1

Journal Information:: New Phytologist, Journal Name: New Phytologist Journal Issue: 2 Vol. 234; ISSN 0028-646X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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79 ASTRONOMY AND ASTROPHYSICS
mycorrhizas
plant phosphorus uptake
rhizosphere modelling
synchrotron
x-ray computed tomography
x-ray fluorescence

Title: Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi

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