Advances in systems metabolic engineering of autotrophic carbon oxide-fixing biocatalysts towards a circular economy

Pavan, Marilene; Reinmets, Kristina; Garg, Shivani; Mueller, Alexander P.; Marcellin, Esteban; Köpke, Michael; Valgepea, Kaspar

doi:10.1016/j.ymben.2022.01.015

Title: Advances in systems metabolic engineering of autotrophic carbon oxide-fixing biocatalysts towards a circular economy

Journal Article · 29 January 2022 · Metabolic Engineering

DOI: https://doi.org/10.1016/j.ymben.2022.01.015 · OSTI ID:1854005

Pavan, Marilene ^[1]; Reinmets, Kristina ^[2]; Garg, Shivani ^[3]; Mueller, Alexander P. ^[3]; Marcellin, Esteban ^[4]; Köpke, Michael ^[3]; Valgepea, Kaspar ^[2]

LanzaTech, Inc., Skokie, IL (United States); Northwestern University
Univ. of Tartu (Estonia)
LanzaTech, Inc., Skokie, IL (United States)
Univ. of Queensland, Brisbane, QLD (Australia)

High levels of anthropogenic CO₂ emissions are driving the warming of global climate. If this pattern of increasing emissions does not change, it will cause further climate change with severe consequences for the human population. On top of this, the increasing accumulation of solid waste within the linear economy model is threatening global biosustainability. The magnitude of these challenges requires several approaches to capture and utilize waste carbon and establish a circular economy. Microbial gas fermentation presents an exciting opportunity to capture carbon oxides from gaseous and solid waste streams with high feedstock flexibility and selectivity. Here we discuss available microbial systems and review in detail the metabolism of both anaerobic acetogens and aerobic hydrogenotrophs and their ability to utilize C1 waste feedstocks. More specifically, we provide an overview of the systems-level understanding of metabolism, key metabolic pathways, scale-up opportunities and commercial successes, and the most recent technological advances in strain and process engineering. Finally, we also discuss in detail the gaps and opportunities to advance the understanding of these autotrophic biocatalysts for the efficient and economically viable production of bioproducts from recycled carbon.

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Research Organization:: LanzaTech, Inc., Skokie, IL (United States)

Sponsoring Organization:: Australian Research Council (ARC); European Research Council (ERC); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018249

OSTI ID:: 1854005

Journal Information:: Metabolic Engineering, Journal Name: Metabolic Engineering Vol. 71; ISSN 1096-7176

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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