Membrane Profiling by Free Flow Electrophoresis and SWATH-MS to Characterize Subcellular Compartment Proteomes in Mesembryanthemum crystallinum
Abstract
The study of subcellular membrane structure and function facilitates investigations into how biological processes are divided within the cell. However, work in this area has been hampered by the limited techniques available to fractionate the different membranes. Free Flow Electrophoresis (FFE) allows for the fractionation of membranes based on their different surface charges, a property made up primarily of their varied lipid and protein compositions. In this study, high-resolution plant membrane fractionation by FFE, combined with mass spectrometry-based proteomics, allowed the simultaneous profiling of multiple cellular membranes from the leaf tissue of the plant Mesembryanthemum crystallinum. Comparisons of the fractionated membranes’ protein profile to that of known markers for specific cellular compartments sheds light on the functions of proteins, as well as provides new evidence for multiple subcellular localization of several proteins, including those involved in lipid metabolism.
- Authors:
-
[1];
[1];
[2];
[1]
- Southern Cross Univ., Lismore, NSW (Australia). Faculty of Science and Engineering. Southern Cross Plant Science
- Univ. of Nevada, Reno, NV (United States). Dept. of Biochemistry and Molecular Biology
- Publication Date:
- Research Org.:
- Univ. of Nevada, Reno, NV (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1851160
- Grant/Contract Number:
- SC0008834
- Resource Type:
- Accepted Manuscript
- Journal Name:
- International Journal of Molecular Sciences (Online)
- Additional Journal Information:
- Journal Name: International Journal of Molecular Sciences (Online); Journal Volume: 22; Journal Issue: 9; Journal ID: ISSN 1422-0067
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; biochemistry & molecular biology; chemistry; subcellular proteomics; membrane fractionation; membrane proteome; marker proteins; subcellular localization; lipid metabolism; lipid biosynthesis; ATPase; mass spectrometry; peptide library
Citation Formats
Guo, Qi, Liu, Lei, Yim, Won C., Cushman, John C., and Barkla, Bronwyn J. Membrane Profiling by Free Flow Electrophoresis and SWATH-MS to Characterize Subcellular Compartment Proteomes in Mesembryanthemum crystallinum. United States: N. p., 2021.
Web. doi:10.3390/ijms22095020.
Guo, Qi, Liu, Lei, Yim, Won C., Cushman, John C., & Barkla, Bronwyn J. Membrane Profiling by Free Flow Electrophoresis and SWATH-MS to Characterize Subcellular Compartment Proteomes in Mesembryanthemum crystallinum. United States. https://doi.org/10.3390/ijms22095020
Guo, Qi, Liu, Lei, Yim, Won C., Cushman, John C., and Barkla, Bronwyn J. Sun .
"Membrane Profiling by Free Flow Electrophoresis and SWATH-MS to Characterize Subcellular Compartment Proteomes in Mesembryanthemum crystallinum". United States. https://doi.org/10.3390/ijms22095020. https://www.osti.gov/servlets/purl/1851160.
@article{osti_1851160,
title = {Membrane Profiling by Free Flow Electrophoresis and SWATH-MS to Characterize Subcellular Compartment Proteomes in Mesembryanthemum crystallinum},
author = {Guo, Qi and Liu, Lei and Yim, Won C. and Cushman, John C. and Barkla, Bronwyn J.},
abstractNote = {The study of subcellular membrane structure and function facilitates investigations into how biological processes are divided within the cell. However, work in this area has been hampered by the limited techniques available to fractionate the different membranes. Free Flow Electrophoresis (FFE) allows for the fractionation of membranes based on their different surface charges, a property made up primarily of their varied lipid and protein compositions. In this study, high-resolution plant membrane fractionation by FFE, combined with mass spectrometry-based proteomics, allowed the simultaneous profiling of multiple cellular membranes from the leaf tissue of the plant Mesembryanthemum crystallinum. Comparisons of the fractionated membranes’ protein profile to that of known markers for specific cellular compartments sheds light on the functions of proteins, as well as provides new evidence for multiple subcellular localization of several proteins, including those involved in lipid metabolism.},
doi = {10.3390/ijms22095020},
journal = {International Journal of Molecular Sciences (Online)},
number = 9,
volume = 22,
place = {United States},
year = {Sun May 09 00:00:00 EDT 2021},
month = {Sun May 09 00:00:00 EDT 2021}
}
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