Ozonized biochar filtrate effects on the growth of Pseudomonas putida and cyanobacteria Synechococcus elongatus PCC 7942

Sacko, Oumar; Engle, Nancy L.; Tschaplinski, Timothy J.; Kumar, Sandeep; Lee, James Weifu

doi:10.1186/s40643-021-00491-2

Title: Ozonized biochar filtrate effects on the growth of Pseudomonas putida and cyanobacteria Synechococcus elongatus PCC 7942

Journal Article · 05 January 2022 · Bioresources and Bioprocessing

DOI: https://doi.org/10.1186/s40643-021-00491-2 · OSTI ID:1847514

Sacko, Oumar ^[1];

^[2];

^[2]; Kumar, Sandeep ^[1];

^[1]

Old Dominion Univ., Norfolk, VA (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Background: Biochar ozonization was previously shown to dramatically increase its cation exchange capacity, thus improving its nutrient retention capacity. The potential soil application of ozonized biochar warrants the need for a toxicity study that investigates its effects on microorganisms. Results: In the study presented here, we found that the filtrates collected from ozonized pine 400 biochar and ozonized rogue biochar did not have any inhibitory effects on the soil environmental bacteria Pseudomonas putida, even at high dissolved organic carbon (DOC) concentrations of 300 ppm. However, the growth of Synechococcus elongatus PCC 7942 was inhibited by the ozonized biochar filtrates at DOC concentrations greater than 75 ppm. Further tests showed the presence of some potential inhibitory compounds (terephthalic acid and p-toluic acid) in the filtrate of non-ozonized pine 400 biochar; these compounds were greatly reduced upon wet-ozonization of the biochar material. Nutrient detection tests also showed that dry-ozonization of rogue biochar enhanced the availability of nitrate and phosphate in its filtrate, a property that may be desirable for soil application. Conclusion: Ozonized biochar substances can support soil environmental bacterium Pseudomonas putida growth, since ozonization detoxifies the potential inhibitory aromatic molecules.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); USDOE Bioenergy Research Centers (BRC) (United States). Center for Bioenergy Innovation (CBI)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1847514

Journal Information:: Bioresources and Bioprocessing, Journal Name: Bioresources and Bioprocessing Journal Issue: 1 Vol. 9; ISSN 2197-4365

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

References (29)

Interactions between plants and beneficial Pseudomonas spp.: exploiting bacterial traits for crop protection Mercado-Blanco, Jesús; Bakker, Peter A. H. M. Antonie van Leeuwenhoek, Vol. 92, Issue 4 https://doi.org/10.1007/s10482-007-9167-1	journal	June 2007
Potential effects of biochar-based microbial inoculants in agriculture Egamberdieva, Dilfuza; Hua, Ma; Reckling, Moritz Environmental Sustainability, Vol. 1, Issue 1 https://doi.org/10.1007/s42398-018-0010-6	journal	March 2018
Decomposition of humic acid and reduction of trihalomethane formation potential in water by ozone with u.v. irradiation Kusakabe, Katsuki; Aso, Shinji; Hayashi, Jun-Ichiro Water Research, Vol. 24, Issue 6 https://doi.org/10.1016/0043-1354(90)90036-6	journal	June 1990
Degradation of wastewater from terephthalic acid manufacturing process by ozonation catalyzed with Fe2+, H2O2 and UV light: Direct versus indirect ozonation reactions Chandrasekara Pillai, K.; Kwon, Tae Ouk; Moon, Il Shik Applied Catalysis B: Environmental, Vol. 91, Issue 1-2 https://doi.org/10.1016/j.apcatb.2009.05.040	journal	September 2009
Investigating the mechanisms of biochar’s removal of lead from solution Wang, Zhenyu; Liu, Guocheng; Zheng, Hao Bioresource Technology, Vol. 177 https://doi.org/10.1016/j.biortech.2014.11.077	journal	February 2015
The use of ozone in the remediation of polycyclic aromatic hydrocarbon contaminated soil O’Mahony, Mark M.; Dobson, Alan D. W.; Barnes, Jeremy D. Chemosphere, Vol. 63, Issue 2 https://doi.org/10.1016/j.chemosphere.2005.07.018	journal	April 2006
Mechanisms of metal sorption by biochars: Biochar characteristics and modifications Li, Hongbo; Dong, Xiaoling; da Silva, Evandro B. Chemosphere, Vol. 178 https://doi.org/10.1016/j.chemosphere.2017.03.072	journal	July 2017
Health risk assessment of heavy metals in freshwater fish in the central and eastern North China Zhong, Wenjue; Zhang, Yanfeng; Wu, Zihao Ecotoxicology and Environmental Safety, Vol. 157 https://doi.org/10.1016/j.ecoenv.2018.03.048	journal	August 2018
Biochar and bacteria inoculated biochar enhanced Cd and Cu immobilization and enzymatic activity in a polluted soil Tu, Chen; Wei, Jing; Guan, Feng Environment International, Vol. 137 https://doi.org/10.1016/j.envint.2020.105576	journal	April 2020
Effect of pyrolysis temperature on potential toxicity of biochar if applied to the environment Lyu, Honghong; He, Yuhe; Tang, Jingchun Environmental Pollution, Vol. 218 https://doi.org/10.1016/j.envpol.2016.08.014	journal	November 2016
Effects and mechanisms of biochar-microbe interactions in soil improvement and pollution remediation: A review Zhu, Xiaomin; Chen, Baoliang; Zhu, Lizhong Environmental Pollution, Vol. 227 https://doi.org/10.1016/j.envpol.2017.04.032	journal	August 2017
Effect of water washing on fuel properties, pyrolysis and combustion characteristics, and ash fusibility of biomass Deng, Lei; Zhang, Tao; Che, Defu Fuel Processing Technology, Vol. 106 https://doi.org/10.1016/j.fuproc.2012.10.006	journal	February 2013
Organic carbon and nutrient release from a range of laboratory-produced biochars and biochar–soil mixtures Mukherjee, Atanu; Zimmerman, Andrew R. Geoderma, Vol. 193-194 https://doi.org/10.1016/j.geoderma.2012.10.002	journal	February 2013
Biochar inoculated with Pseudomonas putida improves grape (Vitis vinifera L.) fruit quality and alters bacterial diversity Wei, Mengmeng; Liu, Xiaochen; He, Yanhui Rhizosphere, Vol. 16 https://doi.org/10.1016/j.rhisph.2020.100261	journal	December 2020
Life in the ‘charosphere’ – Does biochar in agricultural soil provide a significant habitat for microorganisms? Quilliam, Richard S.; Glanville, Helen C.; Wade, Stephen C. Soil Biology and Biochemistry, Vol. 65 https://doi.org/10.1016/j.soilbio.2013.06.004	journal	October 2013
Effects of Water Washing and Torrefaction Pretreatments on Corn Stalk Pyrolysis: Combined Study Using TG-FTIR and a Fixed Bed Reactor Cen, Kehui; Chen, Dengyu; Wang, Jiayang Energy & Fuels, Vol. 30, Issue 12 https://doi.org/10.1021/acs.energyfuels.6b02813	journal	December 2016
Sustainable Chemistry: Solubilization of Phosphorus from Insoluble Phosphate Material Hydroxyapatite with Ozonized Biochar Sacko, Oumar; Whiteman, Rachel; Kharel, Gyanendra ACS Sustainable Chemistry & Engineering, Vol. 8, Issue 18 https://doi.org/10.1021/acssuschemeng.0c00601	journal	April 2020
Investigation into the Sources of Biochar Water-Soluble Organic Compounds and Their Potential Toxicity on Aquatic Microorganisms Smith, Cameron R.; Hatcher, Patrick G.; Kumar, Sandeep ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 5 https://doi.org/10.1021/acssuschemeng.5b01687	journal	April 2016
Biochar Surface Oxygenation by Ozonization for Super High Cation Exchange Capacity Kharel, Gyanendra; Sacko, Oumar; Feng, Xu ACS Sustainable Chemistry & Engineering, Vol. 7, Issue 19 https://doi.org/10.1021/acssuschemeng.9b03536	journal	September 2019
When Wavelengths Collide: Bias in Cell Abundance Measurements Due to Expressed Fluorescent Proteins Hecht, Ariel; Endy, Drew; Salit, Marc ACS Synthetic Biology, Vol. 5, Issue 9 https://doi.org/10.1021/acssynbio.6b00072	journal	June 2016
Quantifying the Total and Bioavailable Polycyclic Aromatic Hydrocarbons and Dioxins in Biochars Hale, Sarah E.; Lehmann, Johannes; Rutherford, David Environmental Science & Technology, Vol. 46, Issue 5 https://doi.org/10.1021/es203984k	journal	February 2012
Molecular Characterization of Inhibiting Biochar Water-Extractable Substances Using Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Smith, Cameron R.; Sleighter, Rachel L.; Hatcher, Patrick G. Environmental Science & Technology, Vol. 47, Issue 23 https://doi.org/10.1021/es4034777	journal	November 2013
Transformation, Morphology, and Dissolution of Silicon and Carbon in Rice Straw-Derived Biochars under Different Pyrolytic Temperatures Xiao, Xin; Chen, Baoliang; Zhu, Lizhong Environmental Science & Technology, Vol. 48, Issue 6 https://doi.org/10.1021/es405676h	journal	March 2014
Occurrence of the potent mutagens 2- nitrobenzanthrone and 3-nitrobenzanthrone in fine airborne particles Santos, Aldenor G.; da Rocha, Gisele O.; de Andrade, Jailson B. Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-018-37186-2	journal	January 2019
Ozonolysis Of Humic Acid And Its Effect On Decoloration And Biodegradability Takahashi, Nobuyuki; Nakai, Toshihiro; Satoh, Yoshio Ozone: Science & Engineering, Vol. 17, Issue 5 https://doi.org/10.1080/01919512.1995.10555764	journal	October 1995
Pseudomonas genomes: diverse and adaptable Silby, Mark W.; Winstanley, Craig; Godfrey, Scott A. C. FEMS Microbiology Reviews, Vol. 35, Issue 4 https://doi.org/10.1111/j.1574-6976.2011.00269.x	journal	July 2011
Down-regulation of the caffeic acid O-methyltransferase gene in switchgrass reveals a novel monolignol analog Tschaplinski, Timothy J.; Standaert, Robert F.; Engle, Nancy L. Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-71	journal	January 2012
Surface oxygenation of biochar through ozonization for dramatically enhancing cation exchange capacity Huff, Matthew D.; Marshall, Sarah; Saeed, Haitham A. Bioresources and Bioprocessing, Vol. 5, Issue 1 https://doi.org/10.1186/s40643-018-0205-9	journal	April 2018
Ozonized biochar filtrate effects on the growth of Pseudomonas putida and cyanobacteria Synechococcus elongatus PCC 7942 Sacko, Oumar; Engle, Nancy L.; Tschaplinski, Timothy J. figshare https://doi.org/10.6084/m9.figshare.c.5781809	collection	January 2022

Linked Research (2)

Similar Records

Engineering quorum-sensing circuits in Synechococcus elongatus PCC 7942 towards self-inducible systems

Journal Article · 2025 · Metabolic Engineering · OSTI ID:2998829

Kokarakis, Emmanuel J.; Santos-Merino, María; Ghaffarinasab, Sajjad; +2 more

Spatial and Temporal Organization of Chromosome Duplication and Segregation in the Cyanobacterium Synechococcus elongatus PCC 7942

Journal Article · 2012 · PLoS ONE · OSTI ID:1904215

Chen, Anna H.; Afonso, Bruno; Silver, Pamela A.; +1 more

Impact of irradiance and inorganic carbon availability on heterologous sucrose production in Synechococcus elongatus PCC 7942

Journal Article · 2024 · Frontiers in Plant Science · OSTI ID:2335543

Yun, Lisa; Zegarac, Robert; Ducat, Daniel C.

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Bioassay
Biological effects of ozonized biochar substances
Dissolved organic carbon
Ozonized biochar filtrate
Pseudomonas putida

Additional file 1 of Ozonized biochar filtrate effects on the growth of Pseudomonas putida and cyanobacteria Synechococcus elongatus PCC 7942 Sacko, Oumar; Engle, Nancy L.; Tschaplinski, Timothy J. https://doi.org/10.6084/m9.figshare.17982200 The current record is supplemented by this text	text	January 2022
Additional file 1 of Ozonized biochar filtrate effects on the growth of Pseudomonas putida and cyanobacteria Synechococcus elongatus PCC 7942 Sacko, Oumar; Engle, Nancy L.; Tschaplinski, Timothy J. https://doi.org/10.6084/m9.figshare.17982200.v1 The current record is supplemented by this text	text	January 2022

Title: Ozonized biochar filtrate effects on the growth of Pseudomonas putida and cyanobacteria Synechococcus elongatus PCC 7942

Citation Formats

References (29)

Linked Research (2)

Similar Records

Related Subjects