Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate

Kuatsjah, Eugene; Johnson, Christopher W.; Salvachúa, Davinia; Werner, Allison Z.; Zahn, Michael; Szostkiewicz, Caralyn J.; Singer, Christine A.; Dominick, Graham; Okekeogbu, Ikenna; Haugen, Stefan J.; Woodworth, Sean P.; Ramirez, Kelsey J.; Giannone, Richard J.; Hettich, Robert L.; McGeehan, John E.; Beckham, Gregg T.

doi:10.1016/j.ymben.2021.12.010

Title: Debottlenecking 4-hydroxybenzoate hydroxylation in Pseudomonas putida KT2440 improves muconate productivity from p-coumarate

Journal Article · 31 December 2021 · Metabolic Engineering

DOI: https://doi.org/10.1016/j.ymben.2021.12.010 · OSTI ID:1847260

Kuatsjah, Eugene ^[1];

^[2];

^[1]; Zahn, Michael ^[3]; Szostkiewicz, Caralyn J. ^[2];

^[2]; Dominick, Graham ^[2]; Okekeogbu, Ikenna ^[4]; Haugen, Stefan J. ^[2]; Woodworth, Sean P. ^[2]; Ramirez, Kelsey J. ^[2]; Giannone, Richard J. ^[4]; Hettich, Robert L. ^[4]; McGeehan, John E. ^[3];

^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Univ. of Portsmouth (United Kingdom)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

The transformation of 4-hydroxybenzoate (4-HBA) to protocatechuate (PCA) is catalyzed by flavoprotein oxygenases known as para-hydroxybenzoate-3-hydroxylases (PHBHs). In Pseudomonas putida KT2440 (P. putida) strains engineered to convert lignin-related aromatic compounds to muconic acid (MA), PHBH activity is rate-limiting, as indicated by the accumulation of 4-HBA, which ultimately limits MA productivity. Here, we hypothesized that replacement of PobA, the native P. putida PHBH, with PraI, a PHBH from Paenibacillus sp. JJ-1b with a broader nicotinamide cofactor preference, could alleviate this bottleneck. Biochemical assays confirmed the strict preference of NADPH for PobA, while PraI can utilize either NADH or NADPH. Kinetic assays demonstrated that both PobA and PraI can utilize NADPH with comparable catalytic efficiency and that PraI also efficiently utilizes NADH at roughly half the catalytic efficiency. The X-ray crystal structure of PraI was solved and revealed absolute conservation of the active site architecture to other PHBH structures despite their differing cofactor preferences. To understand the effect in vivo, we compared three P. putida strains engineered to produce MA from p-coumarate (pCA), showing that expression of praI leads to lower 4-HBA accumulation and decreased NADP+/NADPH ratios relative to strains harboring pobA, indicative of a relieved 4-HBA bottleneck due to increased NADPH availability. In bioreactor cultivations, a strain exclusively expressing praI achieved a titer of 40 g/L MA at 100% molar yield and a productivity of 0.5 g/L/h. Altogether, this study demonstrates the benefit of sampling readily available natural enzyme diversity for debottlenecking metabolic flux in an engineered strain for microbial conversion of lignin-derived compounds to value-added products.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDOE Office of Science (SC), Biological and Environmental Research (BER); Research England

Grant/Contract Number:: AC36-08GO28308; AC05-00OR22725

OSTI ID:: 1847260

Report Number(s):: NREL/JA-2A00-81795; MainId:82568; UUID:848057a4-eac1-4a8e-88c2-c2de0794896e; MainAdminID:63939

Journal Information:: Metabolic Engineering, Journal Name: Metabolic Engineering Vol. 70; ISSN 1096-7176

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
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PraI
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flavoprotei
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muconic acid
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