Quantification of Metabolic Products from Microbial Hosts in Complex Media Using Optically Diffracting Hydrogels

Jung, Sukwon; MacConaghy, Kelsey I.; Guarnieri, Michael T.; Kaar, Joel L.; Stoykovich, Mark P.

doi:10.1021/acsabm.1c01267

Title: Quantification of Metabolic Products from Microbial Hosts in Complex Media Using Optically Diffracting Hydrogels

Journal Article · 15 February 2022 · ACS Applied Bio Materials

DOI: https://doi.org/10.1021/acsabm.1c01267 · OSTI ID:1847080

Jung, Sukwon ^[1]; MacConaghy, Kelsey I. ^[1];

^[2];

^[1]; Stoykovich, Mark P. ^[3]

Univ. of Colorado, Boulder, CO (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States). Biosciences Center
Univ. of Chicago, IL (United States)

In this work, we herein describe a highly versatile platform approach for the in situ and real-time screening of microbial biocatalysts for enhanced production of bioproducts using photonic crystal hydrogels. This approach was demonstrated by preparing optically diffracting films based on polymerized N-isopropylacrylamide that contracted in the presence of alcohols and organic acids. The hydrogel films were prepared in a microwell plate format, which allows for high-throughput screening, and characterized optically using a microwell plate reader. While demonstrating the ability to detect a broad range of relevant alcohols and organic acids, we showed that the response of the films correlated strongly with the octanol-water partition coefficient (log P) of the analyte. Differences in the secretion of ethanol and succinic acid from strains of Zymomonas mobilis and Actinobacillus succinogenes, respectively, were further detected via optical characterization of the films. These differences, which in some cases were as low as ~3 g/L, were confirmed by high-performance liquid chromatography, thereby demonstrating the sensitivity of this approach. Our findings highlight the potential utility of this multiplexed approach for the detection of small organic analytes in complex biological media, which overcomes a major challenge in conventional optical sensing methods.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1847080

Report Number(s):: NREL/JA-2700-82098; MainId:82871; UUID:0d03c430-ea13-4a09-b135-8fe77870929d; MainAdminID:63894

Journal Information:: ACS Applied Bio Materials, Journal Name: ACS Applied Bio Materials Journal Issue: 3 Vol. 5; ISSN 2576-6422

Publisher:: ACS PublicationsCopyright Statement

Country of Publication:: United States

Language:: English

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09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
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organic acids
photonic crystal
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synthetic biology

Title: Quantification of Metabolic Products from Microbial Hosts in Complex Media Using Optically Diffracting Hydrogels

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