Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale

Liew, Fungmin Eric; Nogle, Robert; Abdalla, Tanus; Rasor, Blake J.; Canter, Christina; Jensen, Rasmus O.; Wang, Lan; Strutz, Jonathan; Chirania, Payal; De Tissera, Sashini; Mueller, Alexander P.; Ruan, Zhenhua; Gao, Allan; Tran, Loan; Engle, Nancy L.; Bromley, Jason C.; Daniell, James; Conrado, Robert; Tschaplinski, Timothy J.; Giannone, Richard J.; Hettich, Robert L.; Karim, Ashty S.; Simpson, Séan D.; Brown, Steven D.; Leang, Ching; Jewett, Michael C.; Köpke, Michael

doi:10.1038/s41587-021-01195-w

Title: Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale

Journal Article · 21 February 2022 · Nature Biotechnology

DOI: https://doi.org/10.1038/s41587-021-01195-w · OSTI ID:1846527

Liew, Fungmin Eric ^[1]; Nogle, Robert ^[1]; Abdalla, Tanus ^[1];

^[2]; Canter, Christina ^[1]; Jensen, Rasmus O. ^[1]; Wang, Lan ^[1]; Strutz, Jonathan ^[1]; Chirania, Payal ^[3]; De Tissera, Sashini ^[1]; Mueller, Alexander P. ^[1]; Ruan, Zhenhua ^[1]; Gao, Allan ^[1]; Tran, Loan ^[1];

^[4]; Bromley, Jason C. ^[1];

^[1]; Conrado, Robert ^[1];

^[4];

^[4] more »

LanzaTech, Inc., Skokie, IL (United States)
Northwestern Univ., Evanston, IL (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
LanzaTech, Inc., Skokie, IL (United States); Northwestern Univ.,Chicago, IL (United States)

Many industrial chemicals that are produced from fossil resources could be manufactured more sustainably through fermentation. In this work, we describe the development of a carbon-negative fermentation route to producing the industrially important chemicals acetone and isopropanol from abundant, low-cost waste gas feedstocks, such as industrial emissions and syngas. Using a combinatorial pathway library approach, we first mined a historical industrial strain collection for superior enzymes that we used to engineer the autotrophic acetogen Clostridium autoethanogenum. Next, we used omics analysis, kinetic modeling and cell-free prototyping to optimize flux. Finally, we scaled-up our optimized strains for continuous production at rates of up to ~3 g/L/h and ~90% selectivity. Life cycle analysis confirmed a negative carbon footprint for the products. Unlike traditional production processes, which result in release of greenhouse gases, our process fixes carbon. These results show that engineered acetogens enable sustainable, high-efficiency, high-selectivity chemicals production. We expect that our approach can be readily adapted to a wide range of commodity chemicals.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Energy Efficiency and Renewable Energy (EERE); National Defense Science and Engineering Graduate Fellowship

Grant/Contract Number:: AC05-00OR22725; EE0007566; SC0018249; AC02-05CH11231

OSTI ID:: 1846527

Journal Information:: Nature Biotechnology, Journal Name: Nature Biotechnology Journal Issue: 3 Vol. 40; ISSN 1087-0156

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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Supplementary dataset and tables for "Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale" Liew, Fungmin (Eric); Nogle, Robert; Abdalla, Tanus Zenodo https://doi.org/10.5281/zenodo.5838305	dataset	January 2022
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