Substrate, temperature, and geographical patterns among nearly 2000 natural yeast isolates
Abstract
Yeasts have broad importance as industrially and clinically relevant microbes and as powerful models for fundamental research, but we are only beginning to understand the roles yeasts play in natural ecosystems. Yeast ecology is often more difficult to study compared to other, more abundant microbes, but growing collections of natural yeast isolates are beginning to shed light on fundamental ecological questions. In this work, we used environmental sampling and isolation to assemble a dataset of 1962 isolates collected from throughout the contiguous United States of America (USA) and Alaska, which were then used to uncover geographic patterns, along with substrate and temperature associations among yeast taxa. We found some taxa, including the common yeasts Torulaspora delbrueckii and Saccharomyces paradoxus, to be repeatedly isolated from multiple sampled regions of the USA, and we classify these as broadly distributed cosmopolitan yeasts. A number of yeast taxon-substrate associations were identified, some of which were novel and some of which support previously reported associations. Further, we found a strong effect of isolation temperature on the phyla of yeasts recovered, as well as for many species. We speculate that substrate and isolation temperature associations reflect the ecological diversity of and niche partitioning by yeast taxa.
- Authors:
-
[3]
- Univ. of Wisconsin, Madison, WI (United States)
- Univ. of Wisconsin, Madison, WI (United States); Stanford Univ., CA (United States)
- Univ. of Wisconsin, Madison, WI (United States); Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
- Univ. of Wisconsin, Madison, WI (United States); Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); New York Univ. (NYU), NY (United States)
- Univ. of Wisconsin, Madison, WI (United States); Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); Duke Univ., Durham, NC (United States)
- Univ. of Wisconsin, Madison, WI (United States); Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); West Carroll High School, Savannah, IL (United States)
- Univ. of Wisconsin, Madison, WI (United States); Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); EAGLE School of Madison Fitchburg, WI (United States)
- Univ. of Wisconsin, Madison, WI (United States); Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); School District of Bonduel, WI (United States)
- Univ. of Wisconsin, Madison, WI (United States); Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); Villanova Univ., PA (United States)
- Publication Date:
- Research Org.:
- Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); National Institutes of Health (NIH)
- OSTI Identifier:
- 1846256
- Grant/Contract Number:
- SC0018409; DEB-1253634; DEB-1442148; DEB-2110403; DGE-1256259; 5T32GM007133
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Yeast
- Additional Journal Information:
- Journal Volume: 39; Journal Issue: 1-2; Journal ID: ISSN 0749-503X
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Ascomycota; Basidiomycota; Saccharomyces; ecology; fungi; yeasts.
Citation Formats
Spurley, William J., Fisher, Kaitlin J., Langdon, Quinn K., Buh, Kelly V., Jarzyna, Martin, Haase, Max A. B., Sylvester, Kayla, Moriarty, Ryan V., Rodriguez, Daniel, Sheddan, Angela, Wright, Sarah, Sorlie, Lisa, Hulfachor, Amanda Beth, Opulente, Dana A., and Hittinger, Chris Todd. Substrate, temperature, and geographical patterns among nearly 2000 natural yeast isolates. United States: N. p., 2021.
Web. doi:10.1002/yea.3679.
Spurley, William J., Fisher, Kaitlin J., Langdon, Quinn K., Buh, Kelly V., Jarzyna, Martin, Haase, Max A. B., Sylvester, Kayla, Moriarty, Ryan V., Rodriguez, Daniel, Sheddan, Angela, Wright, Sarah, Sorlie, Lisa, Hulfachor, Amanda Beth, Opulente, Dana A., & Hittinger, Chris Todd. Substrate, temperature, and geographical patterns among nearly 2000 natural yeast isolates. United States. https://doi.org/10.1002/yea.3679
Spurley, William J., Fisher, Kaitlin J., Langdon, Quinn K., Buh, Kelly V., Jarzyna, Martin, Haase, Max A. B., Sylvester, Kayla, Moriarty, Ryan V., Rodriguez, Daniel, Sheddan, Angela, Wright, Sarah, Sorlie, Lisa, Hulfachor, Amanda Beth, Opulente, Dana A., and Hittinger, Chris Todd. Fri .
"Substrate, temperature, and geographical patterns among nearly 2000 natural yeast isolates". United States. https://doi.org/10.1002/yea.3679. https://www.osti.gov/servlets/purl/1846256.
@article{osti_1846256,
title = {Substrate, temperature, and geographical patterns among nearly 2000 natural yeast isolates},
author = {Spurley, William J. and Fisher, Kaitlin J. and Langdon, Quinn K. and Buh, Kelly V. and Jarzyna, Martin and Haase, Max A. B. and Sylvester, Kayla and Moriarty, Ryan V. and Rodriguez, Daniel and Sheddan, Angela and Wright, Sarah and Sorlie, Lisa and Hulfachor, Amanda Beth and Opulente, Dana A. and Hittinger, Chris Todd},
abstractNote = {Yeasts have broad importance as industrially and clinically relevant microbes and as powerful models for fundamental research, but we are only beginning to understand the roles yeasts play in natural ecosystems. Yeast ecology is often more difficult to study compared to other, more abundant microbes, but growing collections of natural yeast isolates are beginning to shed light on fundamental ecological questions. In this work, we used environmental sampling and isolation to assemble a dataset of 1962 isolates collected from throughout the contiguous United States of America (USA) and Alaska, which were then used to uncover geographic patterns, along with substrate and temperature associations among yeast taxa. We found some taxa, including the common yeasts Torulaspora delbrueckii and Saccharomyces paradoxus, to be repeatedly isolated from multiple sampled regions of the USA, and we classify these as broadly distributed cosmopolitan yeasts. A number of yeast taxon-substrate associations were identified, some of which were novel and some of which support previously reported associations. Further, we found a strong effect of isolation temperature on the phyla of yeasts recovered, as well as for many species. We speculate that substrate and isolation temperature associations reflect the ecological diversity of and niche partitioning by yeast taxa.},
doi = {10.1002/yea.3679},
journal = {Yeast},
number = 1-2,
volume = 39,
place = {United States},
year = {2021},
month = {11}
}
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