Functional and Structural Characterization of Diverse NfsB Chloramphenicol Reductase Enzymes from Human Pathogens

Mullowney, Michael W.; Maltseva, Natalia I.; Endres, Michael; Kim, Youngchang; Joachimiak, Andrzej; Crofts, Terence S.; Culyba, ed., Matthew J.

doi:10.1128/spectrum.00139-22

Title: Functional and Structural Characterization of Diverse NfsB Chloramphenicol Reductase Enzymes from Human Pathogens

Journal Article · 22 February 2022 · Microbiology Spectrum

DOI: https://doi.org/10.1128/spectrum.00139-22 · OSTI ID:1846232

^[1];

^[2]; Endres, Michael ^[3];

^[2];

^[4];

^[5]; Culyba, ed., Matthew J.

Department of Chemistry, Northwestern University, Evanston, Illinois, USA
Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago, Chicago, Illinois, USA, Structure Biology Center, Argonne National Laboratory, Argonne, Illinois, USA
Structure Biology Center, Argonne National Laboratory, Argonne, Illinois, USA
Center for Structural Genomics of Infectious Diseases, Consortium for Advanced Science and Engineering, University of Chicago, Chicago, Illinois, USA, Structure Biology Center, Argonne National Laboratory, Argonne, Illinois, USA, Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA
Department of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA

The question of how new enzyme activities evolve is of great biological interest and, in the context of antibiotic resistance, of great medical importance. Here, we have tested the hypothesis that new antibiotic resistance mechanisms may evolve from promiscuous housekeeping enzymes that have antibiotic modification side activities.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: NONE; AC02-06CH11357

OSTI ID:: 1846232

Alternate ID(s):: OSTI ID: 1896795

Journal Information:: Microbiology Spectrum, Journal Name: Microbiology Spectrum; ISSN 2165-0497

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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