Facile Labeling of Sieve Element Phloem-Protein Bodies Using the Reciprocal Oligosaccharide Probe OGA488

Journal Article · 09 February 2022 · Frontiers in Plant Science

DOI: https://doi.org/10.3389/fpls.2022.809923 · OSTI ID:1844458

Azizpor, Pakeeza; Sullivan, Lucy; Lim, Aedric; Groover, Andrew

Sieve elements of many angiosperms contain structural phloem proteins (P-proteins) that can interact to create large P-protein bodies. P-protein bodies can occlude sieve plates upon injury but the range of functional and physiological roles of P-proteins remains uncertain, in part because of challenges in labeling and visualization methods. Here, we show that a reciprocal oligosaccharide probe, OGA ⁴⁸⁸ , can be used in rapid and sensitive labeling of P-protein bodies in Arabidopsis, poplar, snap bean and cucumber in histological sections. OGA ⁴⁸⁸ labeling of knockouts of the two Arabidopsis P-protein-encoding genes, AtSEOR1 and AtSEOR2 , indicated that labeling is specific to AtSEOR2. That protein bodies were labeled and visible in Atseor1 knockouts indicates that heterodimerization of AtSEOR1 and AtSEOR2 may not be necessary for P-protein body formation. Double labeling with a previously characterized stain for P-proteins, sulphorhodamine 101, confirmed P-protein labeling and also higher specificity of OGA ⁴⁸⁸ for P-proteins. OGA ⁴⁸⁸ is thus robust and easily used to label P-proteins in histological sections of multiple angiosperm species.

• Research Organization: USDA Forest Service, Davis, CA (United States); University of Utah, Salt Lake City, UT (United States)
• Sponsoring Organization: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)
• Grant/Contract Number: SC0021996
• OSTI ID: 1844458
• Journal Information: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 13; ISSN 1664-462X
• Publisher: Frontiers Media SACopyright Statement
• Country of Publication: Switzerland
• Language: English