Heterologous expression of phosphite dehydrogenase in the chloroplast or nucleus enables phosphite utilization and genetic selection in Picochlorum spp.

Dahlin, Lukas R.; Guarnieri, Michael T.

doi:10.1016/j.algal.2021.102604

Title: Heterologous expression of phosphite dehydrogenase in the chloroplast or nucleus enables phosphite utilization and genetic selection in Picochlorum spp.

Journal Article · 23 January 2022 · Algal Research

DOI: https://doi.org/10.1016/j.algal.2021.102604 · OSTI ID:1844203

Dahlin, Lukas R. ^[1];

^[1]

National Renewable Energy Lab. (NREL), Golden, CO (United States). Biosciences Center

Microalgae present a path to ameliorate problems associated with climate change via capture and reduction of CO2 to sustainable fuels and chemicals. Picochlorum is a genus of algae recently recognized for potential application in these regards due to its high productivity, thermotolerance, and halotolerance. Foundational genetic tools have recently been established in this genus. However, at present, genetic markers are limited, hindering genetic throughput and trait stacking approaches. To expand the suite of genetic tools and markers available for this genus, we sought to heterologously express the phosphite dehydrogenase (ptxD) gene from Pseudomonas stutzeri WM88 in both the nucleus and chloroplast of Picochlorum renovo and Picochlorum celeri. Additionally, the resultant strains allow for utilization of phosphite as a sole phosphorous source and as a nuclear and plastidial selection marker for genetic engineering. Growth analysis indicated comparable growth and composition when transgenic algae were grown in media containing phosphite as a sole phosphorus source, as compared to the conventionally used phosphate. Combined, these results expand the genetic toolbox available to the Picochlorum genus and present a potential crop protection and biocontainment strategy.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1844203

Report Number(s):: NREL/JA-2700-80931; MainId:78709; UUID:831c2102-7c3a-40f6-b0ac-74a1e4c44b2a; MainAdminID:63807

Journal Information:: Algal Research, Journal Name: Algal Research Vol. 62; ISSN 2211-9264

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
Picochlorum
chloroplast transformation
genetic engineering
microalgae
phosphite
phosphite dehydrogenase
ptxD

Title: Heterologous expression of phosphite dehydrogenase in the chloroplast or nucleus enables phosphite utilization and genetic selection in Picochlorum spp.

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