Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins

Sproles, Ashley E.; Berndt, Anthony; Fields, Francis J.; Mayfield, Stephen P.

doi:10.1007/s00253-022-11790-9

Title: Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins

Journal Article · 06 February 2022 · Applied Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s00253-022-11790-9 · OSTI ID:1843877

Sproles, Ashley E.; Berndt, Anthony; Fields, Francis J.; Mayfield, Stephen P.

Abstract The single-celled eukaryotic green alga Chlamydomonas reinhardtii has long been a model system for developing genetic tools for algae, and is also considered a potential platform for the production of high-value recombinant proteins. Identifying transformants with high levels of recombinant protein expression has been a challenge in this organism, as random integration of transgenes into the nuclear genome leads to low frequency of cell lines with high gene expression. Here, we describe the design of an optimized vector for the expression of recombinant proteins in Chlamydomonas , that when transformed and screened using a dual antibiotic selection, followed by screening using fluorescence activated cell sorting (FACS), permits rapid identification and isolation of microalgal transformants with high expression of a recombinant protein. This process greatly reduces the time required for the screening process, and can produce large populations of recombinant algae transformants with between 60 and 100% of cells producing the recombinant protein of interest, in as little as 3 weeks, that can then be used for whole population sequencing or individual clone analysis. Utilizing this new vector and high-throughput screening (HTS) process resulted in an order of magnitude improvement over existing methods, which normally produced under 1% of algae transformants expressing the protein of interest. This process can be applied to other algal strains and recombinant proteins to enhance screening efficiency, thereby speeding up the discovery and development of algal-derived recombinant protein products. Key points • A protein expression vector using double-antibiotic resistance genes was designed • Double antibiotic selection causes fewer colonies with more positive for phenotype • Coupling the new vector with FACS improves microalgal screening efficiency > 60%

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Research Organization:: Univ. of California, San Diego, CA (United States)

Sponsoring Organization:: USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: EE0008246; EE0008491

OSTI ID:: 1843877

Journal Information:: Applied Microbiology and Biotechnology, Journal Name: Applied Microbiology and Biotechnology Journal Issue: 4 Vol. 106; ISSN 0175-7598

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Germany

Language:: English

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