Effect of microalgae cell composition and size on responsiveness to ultrasonic harvesting

Aligata, Alyssa J.; Tryner, Jessica; Quinn, Jason C.; Marchese, Anthony J.

doi:10.1007/s10811-018-1682-0

Title: Effect of microalgae cell composition and size on responsiveness to ultrasonic harvesting

Journal Article · 13 November 2018 · Journal of Applied Phycology

DOI: https://doi.org/10.1007/s10811-018-1682-0 · OSTI ID:1843738

Aligata, Alyssa J. ^[1]; Tryner, Jessica ^[2]; Quinn, Jason C. ^[2];

^[2]

Colorado State Univ., Fort Collins, CO (United States); Colorado School of Mines
Colorado State Univ., Fort Collins, CO (United States)

Ultrasonic harvesting could reduce the energy consumption and costs associated with separating microalgae from growth media. The responsiveness of microalgae cells to an ultrasonic standing wave depends on the cell radius and acoustic contrast factor (ACF). The ACF can vary as cell composition (e.g. lipid, protein, carbohydrate content) varies depending on the algae strain, cultivation conditions, and growth stage. Here, two independent experimental methods were used to characterize the ACF of three algae ;strains—Nannochloropsis salina, Chlamydomonas reinhardtii, and Tetraselmis chuii—as a function of dynamic cellular composition over 9- to 14-day growth periods. For N. salina, lipid content increased from 25 ± 1% to 33 ± 1% ash-free dry weight (AFDW) and ACF decreased by 46% (from 0.041 ± 0.002 to 0.022 ± 0.002) between growth days 3 and 10. For C. reinhardtii, lipid content increased from 26 ± 1% to 40 ± 1% AFDW and ACF decreased by 33% (from 0.051 ± 0.013 to 0.034 ± 0.006) between growth days 3 and 9. For T. chuii, lipid content and ACF remained stable (~ 10% AFDW and ~ 0.3) over the growth period. ACF decreased as lipid content increased because lipids have a negative ACF in the growth media; however, cell size had a greater impact on cell responsiveness because the ratio of the acoustic radiation force to the drag force is proportional to cell radius squared.

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Research Organization:: Colorado School of Mines, Golden, CO (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: EE0007089

OSTI ID:: 1843738

Journal Information:: Journal of Applied Phycology, Journal Name: Journal of Applied Phycology Journal Issue: 3 Vol. 31; ISSN 0921-8971

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

References (45)

Evaluation of electro-coagulation-flocculation for harvesting marine and freshwater microalgae Vandamme, Dries; Pontes, Sandra Cláudia Vieira; Goiris, Koen Biotechnology and Bioengineering, Vol. 108, Issue 10 https://doi.org/10.1002/bit.23199	journal	May 2011
An ultrasonically enhanced inclined settler for microalgae harvesting Hincapié Gómez, Esteban; Marchese, Anthony J. Biotechnology Progress, Vol. 31, Issue 2 https://doi.org/10.1002/btpr.2031	journal	December 2014
Ion metabolism in a halophilic blue-green alga, Aphanothece halophytica Miller, Donald M.; Jones, Jay H.; Yopp, John H. Archives of Microbiology, Vol. 111, Issue 1-2 https://doi.org/10.1007/BF00446561	journal	January 1976
Viscous effects in the acoustic manipulation of algae for biofuel production Leckey, Cara A. C.; Hinders, Mark K. Journal of Applied Phycology, Vol. 24, Issue 1 https://doi.org/10.1007/s10811-011-9662-7	journal	March 2011
High-value products from microalgae—their development and commercialisation Borowitzka, Michael A. Journal of Applied Phycology, Vol. 25, Issue 3 https://doi.org/10.1007/s10811-013-9983-9	journal	January 2013
Life cycle analysis of algae biodiesel Sander, Kyle; Murthy, Ganti S. The International Journal of Life Cycle Assessment, Vol. 15, Issue 7 https://doi.org/10.1007/s11367-010-0194-1	journal	June 2010
Rapid Quantitative Analysis of Lipids Using a Colorimetric Method in a Microplate Format Cheng, Yu-Shen; Zheng, Yi; VanderGheynst, Jean S. Lipids, Vol. 46, Issue 1 https://doi.org/10.1007/s11745-010-3494-0	journal	November 2010
Current Large-Scale US Biofuel Potential from Microalgae Cultivated in Photobioreactors Quinn, Jason C.; Catton, Kimberly; Wagner, Nicholas BioEnergy Research, Vol. 5, Issue 1 https://doi.org/10.1007/s12155-011-9165-z	journal	November 2011
Ultrasonic velocity as a probe of emulsions and suspensions McClements, David Julian; Povey, Malcolm James William Advances in Colloid and Interface Science, Vol. 27, Issue 3-4 https://doi.org/10.1016/0001-8686(87)85005-4	journal	July 1987
Review of the folin phenol protein quantitation method of lowry, rosebrough, farr and randall Peterson, Gary L. Analytical Biochemistry, Vol. 100, Issue 2 https://doi.org/10.1016/0003-2697(79)90222-7	journal	December 1979
The amino-acid and sugar composition of 16 species of microalgae used in mariculture Brown, Malcolm R. Journal of Experimental Marine Biology and Ecology, Vol. 145, Issue 1 https://doi.org/10.1016/0022-0981(91)90007-J	journal	March 1991
Lipid and biomass production by the halotolerant microalga Nannochloropsis salina Boussiba, Sammy; Vonshak, Avigad; Cohen, Zvi Biomass, Vol. 12, Issue 1 https://doi.org/10.1016/0144-4565(87)90006-0	journal	January 1987
Protein Measurement with the Folin Phenol Reagent Lowry, OliverH.; Rosebrough, NiraJ.; Farr, A. Lewis Journal of Biological Chemistry, Vol. 193, Issue 1 https://doi.org/10.1016/S0021-9258(19)52451-6	journal	November 1951
Changes in biochemical profile of Tetraselmis gracilis I. Comparison of two culture media Lourenço, Sergio O.; Marquez, Ursula M. Lanfer; Mancini-Filho, Jorge Aquaculture, Vol. 148, Issue 2-3 https://doi.org/10.1016/S0044-8486(96)01416-0	journal	January 1997
Getting to low-cost algal biofuels: A monograph on conventional and cutting-edge harvesting and extraction technologies Coons, James E.; Kalb, Daniel M.; Dale, Taraka Algal Research, Vol. 6 https://doi.org/10.1016/j.algal.2014.08.005	journal	October 2014
Energy and operating cost assessment of competing harvesting methods for D. salina in a β-carotene production process Pirwitz, Kristin; Flassig, Robert J.; Rihko-Struckmann, Liisa K. Algal Research, Vol. 12 https://doi.org/10.1016/j.algal.2015.08.016	journal	November 2015
Direct transesterification of wet microalgal biomass for preparation of biodiesel Suh, William I.; Mishra, Sanjiv K.; Kim, Tae-Hyoung Algal Research, Vol. 12 https://doi.org/10.1016/j.algal.2015.10.006	journal	November 2015
Quantification of effects of flue gas derived inorganic contaminants on microalgae growth system and end fate of contaminants Hess, Derek; Napan, Katerine; McNeil, Brian T. Algal Research, Vol. 25 https://doi.org/10.1016/j.algal.2017.04.007	journal	July 2017
Measurement of acoustic properties of microalgae and implications for the performance of ultrasonic harvesting systems Hincapié Gómez, Esteban; Tryner, Jessica; Aligata, Alyssa J. Algal Research, Vol. 31 https://doi.org/10.1016/j.algal.2018.01.015	journal	April 2018
Protein measurements of microalgal and cyanobacterial biomass González López, Cynthia Victoria; García, María del Carmen Cerón; Fernández, Francisco Gabriel Acién Bioresource Technology, Vol. 101, Issue 19 https://doi.org/10.1016/j.biortech.2010.04.077	journal	October 2010
Potential yields and properties of oil from the hydrothermal liquefaction of microalgae with different biochemical content Biller, P.; Ross, A. B. Bioresource Technology, Vol. 102, Issue 1 https://doi.org/10.1016/j.biortech.2010.06.028	journal	January 2011
Renewable fuels from algae: An answer to debatable land based fuels Singh, Anoop; Nigam, Poonam Singh; Murphy, Jerry D. Bioresource Technology, Vol. 102, Issue 1 https://doi.org/10.1016/j.biortech.2010.06.032	journal	January 2011
Nannochloropsis production metrics in a scalable outdoor photobioreactor for commercial applications Quinn, Jason C.; Yates, Tracy; Douglas, Nathaniel Bioresource Technology, Vol. 117, p. 164-171 https://doi.org/10.1016/j.biortech.2012.04.073	journal	August 2012
Effect of Conway Medium and f/2 Medium on the growth of six genera of South China Sea marine microalgae Lananan, Fathurrahman; Jusoh, Ahmad; Ali, Nora’aini Bioresource Technology, Vol. 141 https://doi.org/10.1016/j.biortech.2013.03.006	journal	August 2013
Enhancement of lipid production in low-starch mutants Chlamydomonas reinhardtii by adaptive laboratory evolution Yu, Shuiyan; Zhao, Quanyu; Miao, Xiaoling Bioresource Technology, Vol. 147 https://doi.org/10.1016/j.biortech.2013.08.069	journal	November 2013
Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method Mishra, Sanjiv K.; Suh, William I.; Farooq, Wasif Bioresource Technology, Vol. 155 https://doi.org/10.1016/j.biortech.2013.12.077	journal	March 2014
The potentials and challenges of algae based biofuels: A review of the techno-economic, life cycle, and resource assessment modeling Quinn, Jason C.; Davis, Ryan Bioresource Technology, Vol. 184 https://doi.org/10.1016/j.biortech.2014.10.075	journal	May 2015
Biodiesel from microalgae Chisti, Yusuf Biotechnology Advances, Vol. 25, Issue 3, p. 294-306 https://doi.org/10.1016/j.biotechadv.2007.02.001	journal	May 2007
A new method for rapid determination of carbohydrate and total carbon concentrations using UV spectrophotometry Albalasmeh, Ammar A.; Berhe, Asmeret Asefaw; Ghezzehei, Teamrat A. Carbohydrate Polymers, Vol. 97, Issue 2 https://doi.org/10.1016/j.carbpol.2013.04.072	journal	September 2013
Importance of algae oil as a source of biodiesel Demirbas, Ayhan; Fatih Demirbas, M. Energy Conversion and Management, Vol. 52, Issue 1 https://doi.org/10.1016/j.enconman.2010.06.055	journal	January 2011
Constraints to commercialization of algal fuels Chisti, Yusuf Journal of Biotechnology, Vol. 167, Issue 3 https://doi.org/10.1016/j.jbiotec.2013.07.020	journal	September 2013
Biodiesel from microalgae beats bioethanol Chisti, Yusuf Trends in Biotechnology, Vol. 26, Issue 3 https://doi.org/10.1016/j.tibtech.2007.12.002	journal	March 2008
Algal Biomass: Occurrence of the Main Inorganic Elements and Simulation of Ash Interactions with Bed Material Lane, Daniel J.; Zevenhoven, Maria; Ashman, Peter J. Energy & Fuels, Vol. 28, Issue 7 https://doi.org/10.1021/ef500989n	journal	May 2014
Determination of biomass dry weight of marine microalgae Zhu, C. J.; Lee, Y. K. Journal of Applied Phycology, Vol. 9, Issue 2, p. 189-194 https://doi.org/10.1023/A:1007914806640	journal	April 1997
Flocculation of algae using chitosan Divakaran, Ravi; Sivasankara Pillai, V. N. Journal of Applied Phycology, Vol. 14, Issue 5, p. 419-422 https://doi.org/10.1023/A:1022137023257	journal	January 2002
Ultrasound, a new separation technique to harvest microalgae Bosma, Rouke; van Spronsen, Wim A.; Tramper, Johannes Journal of Applied Phycology, Vol. 15, Issue 2-3, p. 143-153 https://doi.org/10.1023/A:1023807011027	journal	March 2003
Acoustofluidics 7: The acoustic radiation force on small particles Bruus, Henrik Lab on a Chip, Vol. 12, Issue 6 https://doi.org/10.1039/c2lc21068a	journal	January 2012
Biovolume Calculation for Pelagic and Benthic Microalgae Hillebrand, Helmut; Dürselen, Claus-Dieter; Kirschtel, David Journal of Phycology, Vol. 35, Issue 2 https://doi.org/10.1046/j.1529-8817.1999.3520403.x	journal	April 1999
A Sound Velocity Method for Determining the Compressibility of Finely Divided Substances Urick, R. J. Journal of Applied Physics, Vol. 18, Issue 11 https://doi.org/10.1063/1.1697584	journal	November 1947
The Variation with Temperature of the Principal Elastic Moduli of NaCl near the Melting Point Hunter, Lloyd Physical Review, Vol. 61, Issue 1-2 https://doi.org/10.1103/PhysRev.61.84	journal	January 1942
The Path to Triacylglyceride Obesity in the sta6 Strain of Chlamydomonas reinhardtii Goodenough, Ursula; Blaby, Ian; Casero, David Eukaryotic Cell, Vol. 13, Issue 5 https://doi.org/10.1128/EC.00013-14	journal	February 2014
Structural Correlates of Cytoplasmic and Chloroplast Lipid Body Synthesis in Chlamydomonas reinhardtii and Stimulation of Lipid Body Production with Acetate Boost Goodson, Carrie; Roth, Robyn; Wang, Zi Teng Eukaryotic Cell, Vol. 10, Issue 12 https://doi.org/10.1128/EC.05242-11	journal	October 2011
Cell exfoliation, separation, and concentration in the field of a standing ultrasonic wave Pashovkin, T. N.; Sadikova, D. G. Acoustical Physics, Vol. 55, Issue 4-5 https://doi.org/10.1134/S1063771009040150	journal	July 2009
Determination of Total Solids and Ash in Algal Biomass: Laboratory Analytical Procedure (LAP) Van Wychen, Stefanie; Laurens, Lieve M. L. https://doi.org/10.2172/1118077	report	January 2016
Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae Napan, Katerine; Hess, Derek; McNeil, Brian Journal of Visualized Experiments, Issue 101 https://doi.org/10.3791/52936	journal	January 2015

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